##gff-version 3
##sequence-region BBa_K315036 1 3154
BBa_K315036	.	sequence_feature	2285	3154	.	+	0	ID=BBa_K315024;Name=BBa_K315024
BBa_K315036	.	sequence_feature	3121	3154	.	+	0	ID=BBa_K315004;Name=BBa_K315004;Parent=BBa_K315024
BBa_K315036	.	engineered_region	2285	3112	.	+	0	ID=BBa_S04467;Name=BBa_S04467;Parent=BBa_K315024
BBa_K315036	.	engineered_region	2389	3112	.	+	0	ID=BBa_S04419;Name=BBa_S04419;Parent=BBa_S04467
BBa_K315036	.	ribosome_entry_site	2389	2400	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04419
BBa_K315036	.	conserved_region	2393	2396	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K315036	.	CDS	2407	3112	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_S04419
BBa_K315036	.	engineered_tag	3088	3112	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K315036	.	CDS	2407	3081	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K315036	.	engineered_region	2285	2380	.	+	0	ID=BBa_K315017;Name=BBa_K315017;Parent=BBa_S04467
BBa_K315036	.	promoter	2285	2338	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K315017
BBa_K315036	.	promoter	2304	2309	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K315036	.	engineered_region	2285	2338	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K315036	.	non_covalent_binding_site	2285	2303	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K315036	.	non_covalent_binding_site	2310	2328	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K315036	.	promoter	2327	2332	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K315036	.	sequence_feature	2347	2380	.	+	0	ID=BBa_K315001;Name=BBa_K315001;Parent=BBa_K315017
BBa_K315036	.	sequence_feature	2360	2367	.	+	0	ID=annotation2070870;Name=Spacer Region;Parent=BBa_K315001
BBa_K315036	.	sequence_feature	2368	2380	.	+	0	ID=annotation2070871;Name=Arm (Inverted Repeat);Parent=BBa_K315001
BBa_K315036	.	sequence_feature	2347	2359	.	+	0	ID=annotation2070869;Name=Arm (Inverted Repeat);Parent=BBa_K315001
BBa_K315036	.	engineered_region	1	2276	.	+	0	ID=BBa_I718008;Name=BBa_I718008
BBa_K315036	.	CDS	1240	2276	.	+	0	ID=BBa_J61047;Name=BBa_J61047;Parent=BBa_I718008
BBa_K315036	.	ribosome_entry_site	1219	1233	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_I718008
BBa_K315036	.	ribosome_entry_site	1219	1233	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K315036	.	engineered_region	1219	1233	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K315036	.	ribosome_entry_site	1226	1230	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K315036	.	promoter	1	1210	.	+	0	ID=BBa_I0500;Name=BBa_I0500;Parent=BBa_I718008
BBa_K315036	.	sequence_feature	1	1079	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_K315036	.	promoter	1080	1210	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_K315036	.	promoter	1043	1114	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
>BBa_K315036
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcat
tttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtgg
tgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaa
aagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtga
tcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgcc
gcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttg
cccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccag
ttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgac
cgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcac
caccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataa
ccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcg
cttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactg
cgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaa
gccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacac
tttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttc
tccatacccgtttttttgggctagctactagagattaaagaggagaaatactagatgtccaatttactgaccgtacacc
aaaatttgcctgcattaccggtcgatgcaacgagtgatgaggttcgcaagaacctgatggacatgttcagggatcgcca
ggcgttttctgagcatacctggaaaatgcttctgtccgtttgccggtcgtgggcggcatggtgcaagttgaataaccgg
aaatggtttcccgcagaacctgaagatgttcgcgattatcttctatatcttcaggcgcgcggtctggcagtaaaaacta
tccagcaacatttgggccagctaaacatgcttcatcgtcggtccgggctgccacgaccaagtgacagcaatgctgtttc
actggttatgcggcggatccgaaaagaaaacgttgatgccggtgaacgtgcaaaacaggctctagcgttcgaacgcact
gatttcgaccaggttcgttcactcatggaaaatagcgatcgctgccaggatatacgtaatctggcatttctggggattg
cttataacaccctgttacgtatagccgaaattgccaggatcagggttaaagatatctcacgtactgacggtgggagaat
gttaatccatattggcagaacgaaaacgctggttagcaccgcaggtgtagagaaggcacttagcctgggggtaactaaa
ctggtcgagcgatggatttccgtctctggtgtagctgatgatccgaataactacctgttttgccgggtcagaaaaaatg
gtgttgccgcgccatctgccaccagccagctatcaactcgcgccctggaagggatttttgaagcaactcatcgattgat
ttacggcgctaaggatgactctggtcagagatacctggcctggtctggacacagtgcccgtgtcggagccgcgcgagat
atggcccgcgctggagtttcaataccggagatcatgcaagctggtggctggaccaatgtaaatattgtcatgaactata
tccgtaacctggatagtgaaacaggggcaatggtgcgcctgctggaagatggcgattaagaatttactagagtccctat
cagtgatagagattgacatccctatcagtgatagagatactgagcactactagagataacttcgtataatgtgtactat
acgaagttattactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttca
aagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtac
ccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtac
ggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatggg
aacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatcta
caaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttcc
accgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcact
acgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaact
ggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgct
taataacgctgatagtgctagtgtagatcgctactagagataacttcgtataagaaaccatatacgaagttat