LOCUS BBa_K315039 3154 bp DNA linear 22-Dec-2024 DEFINITION pBad + RBS + cre + pTet + lox5171 forward + RBS + RFP + loxBri forward ACCESSION BBa_K315039 VERSION BBa_K315039.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 RBS 1219..1233 /label=RBS-1\Strong RBS 1226..1230 /label=RBS misc_feature 1219..1233 /label=BBa_B0030 RBS 1219..1233 /label=BBa_B0030 CDS 1240..2276 /label=BBa_J61047 misc_feature 1..2276 /label=BBa_I718008 misc_feature 2285..2303 /label=TetR 1 misc_feature 2285..2338 /label=BBa_R0040 promoter 2304..2309 /label=-35 misc_feature 2310..2328 /label=TetR 2 promoter 2327..2332 /label=-10 promoter 2285..2338 /label=BBa_R0040 misc_feature 2347..2359 /label=Arm (Inverted Repeat) misc_feature 2360..2367 /label=Spacer Region misc_feature 2368..2380 /label=Arm (Inverted Repeat) misc_feature 2347..2380 /label=BBa_K315001 misc_feature 2285..2380 /label=BBa_K315017 misc_feature 2393..2396 /label=conserved RBS 2389..2400 /label=BBa_B0034 CDS 2407..3081 /label=mrfp1 misc_feature 3088..3112 /label=Help:Barcodes CDS 2407..3112 /label=BBa_E1010 misc_feature 2389..3112 /label=BBa_S04419 misc_feature 2285..3112 /label=BBa_S04467 misc_feature 3121..3133 /label=inverted repeat arm misc_feature 3134..3141 /label=spacer region misc_feature 3142..3154 /label=inverted repeat arm misc_feature 3135..3135 /label=T -> A misc_feature 3136..3136 /label=G -> C misc_feature 3140..3140 /label=G -> A misc_feature 3121..3154 /label=BBa_K315012 misc_feature 2285..3154 /label=BBa_K315028 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagat taaagaggag aaatactaga tgtccaattt actgaccgta 1261 caccaaaatt tgcctgcatt accggtcgat gcaacgagtg atgaggttcg caagaacctg 1321 atggacatgt tcagggatcg ccaggcgttt tctgagcata cctggaaaat gcttctgtcc 1381 gtttgccggt cgtgggcggc atggtgcaag ttgaataacc ggaaatggtt tcccgcagaa 1441 cctgaagatg ttcgcgatta tcttctatat cttcaggcgc gcggtctggc agtaaaaact 1501 atccagcaac atttgggcca gctaaacatg cttcatcgtc ggtccgggct gccacgacca 1561 agtgacagca atgctgtttc actggttatg cggcggatcc gaaaagaaaa cgttgatgcc 1621 ggtgaacgtg caaaacaggc tctagcgttc gaacgcactg atttcgacca ggttcgttca 1681 ctcatggaaa atagcgatcg ctgccaggat atacgtaatc tggcatttct ggggattgct 1741 tataacaccc tgttacgtat agccgaaatt gccaggatca gggttaaaga tatctcacgt 1801 actgacggtg ggagaatgtt aatccatatt ggcagaacga aaacgctggt tagcaccgca 1861 ggtgtagaga aggcacttag cctgggggta actaaactgg tcgagcgatg gatttccgtc 1921 tctggtgtag ctgatgatcc gaataactac ctgttttgcc gggtcagaaa aaatggtgtt 1981 gccgcgccat ctgccaccag ccagctatca actcgcgccc tggaagggat ttttgaagca 2041 actcatcgat tgatttacgg cgctaaggat gactctggtc agagatacct ggcctggtct 2101 ggacacagtg cccgtgtcgg agccgcgcga gatatggccc gcgctggagt ttcaataccg 2161 gagatcatgc aagctggtgg ctggaccaat gtaaatattg tcatgaacta tatccgtaac 2221 ctggatagtg aaacaggggc aatggtgcgc ctgctggaag atggcgatta agaatttact 2281 agagtcccta tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacta 2341 ctagagataa cttcgtataa tgtgtactat acgaagttat tactagagaa agaggagaaa 2401 tactagatgg cttcctccga agacgttatc aaagagttca tgcgtttcaa agttcgtatg 2461 gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac 2521 gaaggtaccc agaccgctaa actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg 2581 gacatcctgt ccccgcagtt ccagtacggt tccaaagctt acgttaaaca cccggctgac 2641 atcccggact acctgaaact gtccttcccg gaaggtttca aatgggaacg tgttatgaac 2701 ttcgaagacg gtggtgttgt taccgttacc caggactcct ccctgcaaga cggtgagttc 2761 atctacaaag ttaaactgcg tggtaccaac ttcccgtccg acggtccggt tatgcagaaa 2821 aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa 2881 ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc actacgacgc tgaagttaaa 2941 accacctaca tggctaaaaa accggttcag ctgccgggtg cttacaaaac cgacatcaaa 3001 ctggacatca cctcccacaa cgaagactac accatcgttg aacagtacga acgtgctgaa 3061 ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct agtgtagatc gctactagag 3121 ataacttcgt ataaactata ctatacgaag ttat //