LOCUS       BBa_K315043             2004 bp    DNA     linear       27-Jun-2024
DEFINITION  pLacI-RBS-RFP-RBS-TetA (Optimized from bp 1-290)
ACCESSION   BBa_K315043
VERSION     BBa_K315043.1  
FEATURES             Location/Qualifiers
     promoter        17..22
                     /label=-35
     promoter        44..49
                     /label=-10
     promoter        1..56
                     /label=BBa_K091110
     misc_feature    69..72
                     /label=conserved
     RBS             65..76
                     /label=BBa_B0034
     CDS             83..757
                     /label=mrfp1
     misc_feature    764..788
                     /label=Help:Barcodes
     CDS             83..788
                     /label=BBa_E1010
     misc_feature    65..788
                     /label=BBa_J70390
     misc_feature    1..788
                     /label=BBa_K315008
     misc_feature    801..804
                     /label=conserved
     RBS             797..808
                     /label=BBa_B0034
     misc_feature    1..808
                     /label=BBa_S04447
     CDS             815..2004
                     /label=BBa_K315040
ORIGIN
        1 cgttgacacc atcgaatggc gcaaaacctt tcgcggtatg gcatgatagc gcccggtact
       61 agagaaagag gagaaatact agatggcttc ctccgaagac gttatcaaag agttcatgcg
      121 tttcaaagtt cgtatggaag gttccgttaa cggtcacgag ttcgaaatcg aaggtgaagg
      181 tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg aaagttacca aaggtggtcc
      241 gctgccgttc gcttgggaca tcctgtcccc gcagttccag tacggttcca aagcttacgt
      301 taaacacccg gctgacatcc cggactacct gaaactgtcc ttcccggaag gtttcaaatg
      361 ggaacgtgtt atgaacttcg aagacggtgg tgttgttacc gttacccagg actcctccct
      421 gcaagacggt gagttcatct acaaagttaa actgcgtggt accaacttcc cgtccgacgg
      481 tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc accgaacgta tgtacccgga
      541 agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg gtggtcacta
      601 cgacgctgaa gttaaaacca cctacatggc taaaaaaccg gttcagctgc cgggtgctta
      661 caaaaccgac atcaaactgg acatcacctc ccacaacgaa gactacacca tcgttgaaca
      721 gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa taacgctgat agtgctagtg
      781 tagatcgcta ctagagaaag aggagaaata ctagatgaaa tctaacaacg cgctgatcgt
      841 tatcctgggt accgttaccc tggacgcggt tggtatcggt ctggttatgc cggttctgcc
      901 gggtctgctg cgtgacatcg ttcactctga ctctatcgcg tctcactacg gtgttctgct
      961 agcctgtacg cgctgatgca gttcctgtgc gcgccggttc tgggtgcgct gtctgaccgt
     1021 ttcggtcgtc gtccggttct gctggcgtct ctgctgggtg cgaccatcga ctacgcgatc
     1081 atggcgacca ccccggttct gtggatcctc tacgccggac gcatcgtggc cggcatcacc
     1141 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg
     1201 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg
     1261 gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc
     1321 aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt
     1381 cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg
     1441 actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg
     1501 gcagcgctct gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc
     1561 ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggcccc
     1621 gccaccaaac gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg
     1681 ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt
     1741 ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat
     1801 gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc
     1861 attggaccgc tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg
     1921 gcatggattg taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca
     1981 tggagccggg ccacctcgac ctaa
//