LOCUS       BBa_K316004             1104 bp    DNA     linear       25-Nov-2024
DEFINITION  Functional XylE under J23101 promoter, with double terminator
ACCESSION   BBa_K316004
VERSION     BBa_K316004.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     RBS             57..62
                     /label=rbs
     CDS             70..993
                     /label=cds
     misc_feature    44..1001
                     /label=BBa_J33204
     stem_loop       1017..1036
                     /label=T7 TE
     misc_feature    1043..1043
                     /label=stop
     polyA_site      1037..1050
                     /label=polya
     misc_feature    1010..1050
                     /label=BBa_B0012
     terminator      1010..1050
                     /label=BBa_B0012
     stem_loop       1071..1093
                     /label=stem_loop
     misc_feature    1059..1104
                     /label=BBa_B0011
     terminator      1059..1104
                     /label=BBa_B0011
     terminator      1010..1104
                     /label=BBa_B0014
     CDS             44..1104
                     /label=BBa_K316003
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagctcatga actatgaaga
       61 ggtgacgtca tgaacaaagg tgtaatgcga ccgggccatg tgcagctgcg tgtactggac
      121 atgagcaagg ccctggaaca ctacgtcgag ttgctgggcc tgatcgagat ggaccgtgac
      181 gaccagggcc gtgtctatct gaaggcttgg accgaagtgg ataagttttc cctggtgcta
      241 cgcgaggctg acgagccggg catggatttt atgggtttca aggttgtgga tgaggatgct
      301 ctccggcaac tggagcggga tctgatggca tatggctgtg ccgttgagca gctacccgca
      361 ggtgaactga acagttgtgg ccggcgcgtg cgcttccagg ccccctccgg gcatcacttc
      421 gagttgtatg cagacaagga atatactgga aagtggggtt tgaatgacgt caatcccgag
      481 gcatggccgc gcgatctgaa aggtatggcg gctgtgcgtt tcgaccacgc cctcatgtat
      541 ggcgacgaat tgccggcgac ctatgacctg ttcaccaagg tgctcggttt ctatctggcc
      601 gaacaggtgc tggacgaaaa tggcacgcgc gtcgcccagt ttctcagtct gtcgaccaag
      661 gcccacgacg tggccttcat tcaccatccg gaaaaaggcc gcctccatca tgtgtccttc
      721 cacctcgaaa cctgggaaga cttgcttcgc gccgccgacc tgatctccat gaccgacaca
      781 tctatcgata tcggcccaac ccgccacggc ctcactcacg gcaagaccat ctacttcttc
      841 gacccgtccg gtaaccgcaa cgaagtgttc tgcgggggag attacaacta cccggaccac
      901 aaaccggtga cctggaccac cgaccagctg ggcaaggcga tcttttacca cgaccgcatt
      961 ctcaacgaac gattcatgac cgtgctgacc tgatggtccg gtactagagt cacactggct
     1021 caccttcggg tgggcctttc tgcgtttata tactagagag agaatataaa aagccagatt
     1081 attaatccgg cttttttatt attt
//