LOCUS       BBa_K316006             1722 bp    DNA     linear       22-Nov-2024
DEFINITION  N-terminus his tagged-GFP-XylE fusion protein
ACCESSION   BBa_K316006
VERSION     BBa_K316006.1  
FEATURES             Location/Qualifiers
     misc_feature    4..21
                     /label=His tag
     misc_feature    1..3
                     /label=Start
     CDS             22..732
                     /label=GFP E0040
     misc_feature    733..756
                     /label=Flag Tag
     misc_feature    757..777
                     /label=Tev Protease Cleavage Site
     misc_feature    778..795
                     /label=GGS linker
     CDS             796..1722
                     /label=XylE J33204
ORIGIN
        1 atgcatcacc atcatcacca tcgtaaagga gaagaacttt tcactggagt tgtcccaatt
       61 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
      121 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
      181 gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac
      241 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
      301 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt
      361 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga
      421 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca
      481 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga
      541 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
      601 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
      661 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
      721 gaactataca aagattataa agatgatgac gacaaggaaa atctttattt tcaaggggga
      781 ggttcaggag gcagcatgaa caaaggtgta atgcgaccgg gccatgtgca gctgcgtgta
      841 ctggacatga gcaaggccct ggaacactac gtcgagttgc tgggcctgat cgagatggac
      901 cgtgacgacc agggccgtgt ctatctgaag gcttggaccg aagtggataa gttttccctg
      961 gtgctacgcg aggctgacga gccgggcatg gattttatgg gtttcaaggt tgtggatgag
     1021 gatgctctcc ggcaactgga gcgggatctg atggcatatg gctgtgccgt tgagcagcta
     1081 cccgcaggtg aactgaacag ttgtggccgg cgcgtgcgct tccaggcccc ctccgggcat
     1141 cacttcgagt tgtatgcaga caaggaatat actggaaagt ggggtttgaa tgacgtcaat
     1201 cccgaggcat ggccgcgcga tctgaaaggt atggcggctg tgcgtttcga ccacgccctc
     1261 atgtatggcg acgaattgcc ggcgacctat gacctgttca ccaaggtgct cggtttctat
     1321 ctggccgaac aggtgctgga cgaaaatggc acgcgcgtcg cccagtttct cagtctgtcg
     1381 accaaggccc acgacgtggc cttcattcac catccggaaa aaggccgcct ccatcatgtg
     1441 tccttccacc tcgaaacctg ggaagacttg cttcgcgccg ccgacctgat ctccatgacc
     1501 gacacatcta tcgatatcgg cccaacccgc cacggcctca ctcacggcaa gaccatctac
     1561 ttcttcgacc cgtccggtaa ccgcaacgaa gtgttctgcg ggggagatta caactacccg
     1621 gaccacaaac cggtgacctg gaccaccgac cagctgggca aggcgatctt ttaccacgac
     1681 cgcattctca acgaacgatt catgaccgtg ctgacctaat aa
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