##gff-version 3
##sequence-region BBa_K316009 1 2830
BBa_K316009	.	terminator	2736	2830	.	+	0	ID=BBa_B0014;Name=BBa_B0014
BBa_K316009	.	terminator	2785	2830	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K316009	.	stem_loop	2797	2819	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K316009	.	engineered_region	2785	2830	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K316009	.	terminator	2736	2776	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K316009	.	engineered_region	2736	2776	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K316009	.	stem_loop	2743	2762	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K316009	.	stop_codon	2769	2769	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K316009	.	polyA_site	2763	2776	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K316009	.	engineered_region	883	999	.	+	0	ID=BBa_K143053;Name=BBa_K143053
BBa_K316009	.	promoter	883	979	.	+	0	ID=BBa_K143012;Name=BBa_K143012;Parent=BBa_K143053
BBa_K316009	.	promoter	945	950	.	+	0	ID=annotation1975704;Name=Sigma A-35;Parent=BBa_K143012
BBa_K316009	.	promoter	968	973	.	+	0	ID=annotation1975705;Name=Sigma A -10;Parent=BBa_K143012
BBa_K316009	.	ribosome_entry_site	988	999	.	+	0	ID=BBa_K143021;Name=BBa_K143021;Parent=BBa_K143053
BBa_K316009	.	ribosome_entry_site	988	999	.	+	0	ID=annotation1975997;Name=rbs;Parent=BBa_K143021
BBa_K316009	.	CDS	1	874	.	+	0	ID=BBa_K316017;Name=BBa_K316017
BBa_K316009	.	CDS	1	717	.	+	0	ID=BBa_K316012;Name=BBa_K316012;Parent=BBa_K316017
BBa_K316009	.	CDS	1	717	.	+	0	ID=annotation2100977;Name=TEV protease;Parent=BBa_K316012
BBa_K316009	.	promoter	726	874	.	+	0	ID=BBa_K316000;Name=BBa_K316000;Parent=BBa_K316017
BBa_K316009	.	sequence_feature	738	745	.	+	0	ID=annotation2097817;Name=scar;Parent=BBa_K316000
BBa_K316009	.	non_covalent_binding_site	836	865	.	+	0	ID=annotation2100979;Name=LacI binding;Parent=BBa_K316000
BBa_K316009	.	ribosome_entry_site	726	737	.	+	0	ID=annotation2097815;Name=sRBS;Parent=BBa_K316000
BBa_K316009	.	non_covalent_binding_site	746	776	.	+	0	ID=annotation2100978;Name=LacI binding;Parent=BBa_K316000
BBa_K316009	.	engineered_region	1006	2727	.	+	0	ID=BBa_K316006;Name=BBa_K316006
BBa_K316009	.	sequence_feature	1783	1800	.	+	0	ID=annotation2094921;Name=GGS linker;Parent=BBa_K316006
BBa_K316009	.	CDS	1801	2727	.	+	0	ID=annotation2094922;Name=XylE J33204;Parent=BBa_K316006
BBa_K316009	.	tag	1009	1026	.	+	0	ID=annotation2094916;Name=His tag;Parent=BBa_K316006
BBa_K316009	.	tag	1738	1761	.	+	0	ID=annotation2094919;Name=Flag Tag;Parent=BBa_K316006
BBa_K316009	.	sequence_feature	1762	1782	.	+	0	ID=annotation2094920;Name=Tev Protease Cleavage Site;Parent=BBa_K316006
BBa_K316009	.	start_codon	1006	1008	.	+	0	ID=annotation2094917;Name=Start;Parent=BBa_K316006
BBa_K316009	.	CDS	1027	1737	.	+	0	ID=annotation2094918;Name=GFP E0040;Parent=BBa_K316006
>BBa_K316009
ttattagttcattaactgtgtggcctctttaaccggttgaaacggttcttccggtttatccatgaacactttgtgacct
ccccaaagaacggaatctgcattcagtctccagcctgaaacccattgttgagcttcttggttcgtcagtaactccatga
agttcttgggaacactcgtaaagtaattattcgtattggtgaagtttgacgcagagtgtatgcccacaataaacccatc
ccgtgttgacacaagaggagagccacattgcccatcttttgtctgaatccaatgcttccaaaagatgccatcagagcta
gggaatgtacagctagtgtctgataccattgaggacatagatttggtctgaaagtttgttgtgactaagcagatccgtt
cttcacgctgaggttcgcgaaacttgagtttctgaggaaacggtggaaagtctttcggcattcgaatgataatcatgtc
tcttccatctatcagatgttgctgaagcgtcgttgtgtttttgaccttaaagactccatgaagggattgtaccaacagt
gtaccattattgcgcctaaagaggtgtttgttcgtgatgatgaatggcccaaagccaatgccatacaagctagtggtat
gaccatcactctcattggtcagatggcaaatagtcgagctaatcggattatagtcacgtggtcctttaaacaacgatct
tcccattactagagttcacctcctttctctagtatgtgaattgttatccgctcacaattccacacacacattatgccac
accttgtagataaagtcaacaacttttgcaactttctcggcaaaatgtggaattgtgagcgctcacaattccacaaccc
tcgagtactagagaattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttga
caaaaatgggctcgtgttgtacaataaatgttactagagaaaggtggtgaatactagatgcatcaccatcatcaccatc
gtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttc
tgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacct
gttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagc
atgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaa
gacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagat
ggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatg
gaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatac
tccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaac
gaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaag
attataaagatgatgacgacaaggaaaatctttattttcaagggggaggttcaggaggcagcatgaacaaaggtgtaat
gcgaccgggccatgtgcagctgcgtgtactggacatgagcaaggccctggaacactacgtcgagttgctgggcctgatc
gagatggaccgtgacgaccagggccgtgtctatctgaaggcttggaccgaagtggataagttttccctggtgctacgcg
aggctgacgagccgggcatggattttatgggtttcaaggttgtggatgaggatgctctccggcaactggagcgggatct
gatggcatatggctgtgccgttgagcagctacccgcaggtgaactgaacagttgtggccggcgcgtgcgcttccaggcc
ccctccgggcatcacttcgagttgtatgcagacaaggaatatactggaaagtggggtttgaatgacgtcaatcccgagg
catggccgcgcgatctgaaaggtatggcggctgtgcgtttcgaccacgccctcatgtatggcgacgaattgccggcgac
ctatgacctgttcaccaaggtgctcggtttctatctggccgaacaggtgctggacgaaaatggcacgcgcgtcgcccag
tttctcagtctgtcgaccaaggcccacgacgtggccttcattcaccatccggaaaaaggccgcctccatcatgtgtcct
tccacctcgaaacctgggaagacttgcttcgcgccgccgacctgatctccatgaccgacacatctatcgatatcggccc
aacccgccacggcctcactcacggcaagaccatctacttcttcgacccgtccggtaaccgcaacgaagtgttctgcggg
ggagattacaactacccggaccacaaaccggtgacctggaccaccgaccagctgggcaaggcgatcttttaccacgacc
gcattctcaacgaacgattcatgaccgtgctgacctaataatactagagtcacactggctcaccttcgggtgggccttt
ctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt