BBa_K316011 1 BBa_K316011 Reverse strand coding XylE 2010-10-25T11:00:00Z 2015-05-08T01:11:56Z This part is a modified existing biobrick <bbpart>BBa_J33204</bbpart>. This part contains XylE coding on the 3' strand. In many biological settings there may be read-through caused by upstream elements, however this can often be unidirectional. In settings where readthrough from 5' direction is expected to be much stronger than 3' direction, it may is advantageous to use 3' coding sequence instead of traditional 5'. For the purposes of characterisation, the part was made on the minus strand to reduce background transcription. Read-through from 5' direction may provide an additional benefit. Low quantities of anti-sence RNA may be produced as the result of 5' readthrough, which may in turn reduce basal expression sometimes seen in LacI systems. false false _440_ 0 7480 9 Not in stock false Part was designed using several rounds of PCR, using Pfu polymerase to avoid mutations. For more information, please check our wiki[http://2010.igem.org/Team:Imperial_College_London/Strategy]. false IC 2010 Team annotation2107116 1 Start Codon range2107116 1 922 924 annotation2107193 1 XylE Gene range2107193 1 4 924 annotation2107181 1 Stop Codon range2107181 1 1 3 BBa_K316011_sequence 1 tcaggtcagcacggtcatgaatcgttcgttgagaatgcggtcgtggtaaaagatcgccttgcccagctggtcggtggtccaggtcaccggtttgtggtccgggtagttgtaatctcccccgcagaacacttcgttgcggttaccggacgggtcgaagaagtagatggtcttgccgtgagtgaggccgtggcgggttgggccgatatcgatagatgtgtcggtcatggagatcaggtcggcggcgcgaagcaagtcttcccaggtttcgaggtggaaggacacatgatggaggcggcctttttccggatggtgaatgaaggccacgtcgtgggccttggtcgacagactgagaaactgggcgacgcgcgtgccattttcgtccagcacctgttcggccagatagaaaccgagcaccttggtgaacaggtcataggtcgccggcaattcgtcgccatacatgagggcgtggtcgaaacgcacagccgccatacctttcagatcgcgcggccatgcctcgggattgacgtcattcaaaccccactttccagtatattccttgtctgcatacaactcgaagtgatgcccggagggggcctggaagcgcacgcgccggccacaactgttcagttcacctgcgggtagctgctcaacggcacagccatatgccatcagatcccgctccagttgccggagagcatcctcatccacaaccttgaaacccataaaatccatgcccggctcgtcagcctcgcgtagcaccagggaaaacttatccacttcggtccaagccttcagatagacacggccctggtcgtcacggtccatctcgatcaggcccagcaactcgacgtagtgttccagggccttgctcatgtccagtacacgcagctgcacatggcccggtcgcattacacctttgttcat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z