LOCUS       BBa_K316020             2070 bp    DNA     linear       25-Nov-2024
DEFINITION  B. subtilis genome integration vector, targets pyrD locus
ACCESSION   BBa_K316020
VERSION     BBa_K316020.1  
FEATURES             Location/Qualifiers
     misc_feature    1..468
                     /label=PyrD 5' Integration Sequence
     misc_feature    1..468
                     /label=BBa_K143008
     misc_feature    477..504
                     /label=BBa_K316002
     promoter        575..580
                     /label=Sigma A-35
     promoter        598..603
                     /label=Sigma A -10
     promoter        513..609
                     /label=BBa_K143012
     RBS             618..629
                     /label=rbs
     RBS             618..629
                     /label=BBa_K143021
     misc_feature    513..629
                     /label=BBa_K143053
     CDS             636..1400
                     /label=Aad9 Spectinomycin Acetyltransferase
     misc_feature    1401..1403
                     /label=stop
     misc_feature    1404..1406
                     /label=stop
     misc_feature    636..638
                     /label=start
     CDS             636..1406
                     /label=BBa_K143031
     stem_loop       1426..1469
                     /label=stem_loop
     misc_feature    1415..1494
                     /label=BBa_B0010
     terminator      1415..1494
                     /label=BBa_B0010
     stem_loop       1510..1529
                     /label=T7 TE
     misc_feature    1536..1536
                     /label=stop
     polyA_site      1530..1543
                     /label=polya
     misc_feature    1503..1543
                     /label=BBa_B0012
     terminator      1503..1543
                     /label=BBa_B0012
     misc_feature    636..1543
                     /label=BBa_K143065
     misc_feature    1552..1579
                     /label=BBa_K316002
     misc_feature    1588..1591
                     /label=5' product
     misc_feature    1592..1595
                     /label=3' product
     misc_feature    1588..1595
                     /label=BBa_K316013
     misc_feature    1552..1595
                     /label=BBa_K316014
     misc_feature    1604..2070
                     /label=PyrD 3' Integration Sequence
     misc_feature    1604..2070
                     /label=BBa_K143009
ORIGIN
        1 atgctagagg tgaaattgcc gggacttgat ttgaaaaacc caatcattcc tgcatcaggc
       61 tgcttcggtt ttggaaaaga attttcacgt ttttatgatt tgtcttgtct tggagctatc
      121 atgattaagg ctacgacaaa ggagccgcgc tttgggaatc cgacgccgcg ggtagctgag
      181 actggtgctg gaatgctcaa tgcgatcggt ctccaaaatc cggggctgga tagtgtgttg
      241 catcatgagc tgccgtggct tgagcagttt gatacaccga tcattgccaa tgtcgcaggt
      301 tctcaagtcg atgattatgt tgaagtcgca gaacatatca gcaaagcgcc taatgttcat
      361 gctcttgaat tgaatatttc ctgcccgaat gtgaaaacag gcggaatcgc ttttggcacg
      421 aatcctgaaa tggctgccga tttgacaaaa gcggtgaaag aggtttcgta ctagagatct
      481 cctagaatat atattatgta aacttactag agaattttgt caaaataatt ttattgacaa
      541 cgtcttatta acgttgatat aatttaaatt ttatttgaca aaaatgggct cgtgttgtac
      601 aataaatgtt actagagaaa ggtggtgaat actagatgag gaggatatat ttgaatacat
      661 acgaacaaat taataaagtg aaaaaaatac ttcggaaaca tttaaaaaat aaccttattg
      721 gtacttacat gtttggatca ggagttgaga gtggactaaa accaaatagt gatcttgact
      781 ttttagtcgt cgtatctgaa ccattgacag atcaaagtaa agaaatactt atacaaaaaa
      841 ttagacctat ttcaaaaaaa ataggagata aaagcaactt acgatatatt gaattaacaa
      901 ttattattca gcaagaaatg gtaccgtgga atcatcctcc caaacaagaa tttatttatg
      961 gagaatggtt acaagagctt tatgaacaag gatacattcc tcagaaggaa ttaaattcag
     1021 atttaaccat aatgctttac caagcaaaac gaaaaaataa aagaatatac ggaaattatg
     1081 acttagagga attactacct gatattccat tttctgatgt gagaagagcc attatggatt
     1141 cgtcagagga attaatagat aattatcagg atgatgaaac caactctata ttaactttat
     1201 gccgtatgat tttaactatg gacacgggta aaatcatacc aaaagatatt gcgggaaatg
     1261 cagtggctga atcttctcca ttagaacata gggagagaat tttgttagca gttcgtagtt
     1321 atcttggaga gaatattgaa tggactaatg aaaatgtaaa tttaactata aactatttaa
     1381 ataacagatt aaaaaaatta taataatact agagccaggc atcaaataaa acgaaaggct
     1441 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
     1501 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gatctcctag
     1561 aatatatatt atgtaaactt actagaggaa atttctacta gaggatgtac ccgtttatgt
     1621 gaagctatcc ccgaacgtgg ctaatatcac agaaattgca ttagcgatcg aggaagcggg
     1681 agcggacggt cttacgatga tcaacacact aatcggcatg agactcgatt taaaaaccgg
     1741 caaaccgata ttagcgaata aaacaggggg actttcgggc cctgctgtga agccggttgc
     1801 cattcgcatg gtgtatgaag tcagccagat ggtcaacatc ccgattatcg gaatgggagg
     1861 cgtgcaaacg gctgaagatg ccctggaatt tcttctcgcg ggagcaagcg cagtcgctgt
     1921 cggaacagca aactttgtga atccttttgc atgtccagag attattgaac agctcccatc
     1981 tgttttgctc caatacggct atcaatcaat tgaagaatgc atcggaagga gctggaatca
     2041 tgaaaaacaa cctgcccatc atcgcgcttg
//