##gff-version 3
##sequence-region BBa_K316020 1 2070
BBa_K316020	.	engineered_region	636	1543	.	+	0	ID=BBa_K143065;Name=BBa_K143065
BBa_K316020	.	CDS	636	1406	.	+	0	ID=BBa_K143031;Name=BBa_K143031;Parent=BBa_K143065
BBa_K316020	.	stop_codon	1404	1406	.	+	0	ID=annotation1992697;Name=stop;Parent=BBa_K143031
BBa_K316020	.	CDS	636	1400	.	+	0	ID=annotation1975961;Name=Aad9 Spectinomycin Acetyltransferase;Parent=BBa_K143031
BBa_K316020	.	start_codon	636	638	.	+	0	ID=annotation1992698;Name=start;Parent=BBa_K143031
BBa_K316020	.	stop_codon	1401	1403	.	+	0	ID=annotation1992696;Name=stop;Parent=BBa_K143031
BBa_K316020	.	terminator	1503	1543	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K143065
BBa_K316020	.	engineered_region	1503	1543	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K316020	.	stop_codon	1536	1536	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K316020	.	polyA_site	1530	1543	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K316020	.	stem_loop	1510	1529	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K316020	.	terminator	1415	1494	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K143065
BBa_K316020	.	stem_loop	1426	1469	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K316020	.	engineered_region	1415	1494	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K316020	.	sequence_feature	1604	2070	.	+	0	ID=BBa_K143009;Name=BBa_K143009
BBa_K316020	.	sequence_feature	1604	2070	.	+	0	ID=annotation1977517;Name=PyrD 3' Integration Sequence;Parent=BBa_K143009
BBa_K316020	.	sequence_feature	477	504	.	+	0	ID=BBa_K316002;Name=BBa_K316002
BBa_K316020	.	engineered_region	513	629	.	+	0	ID=BBa_K143053;Name=BBa_K143053
BBa_K316020	.	promoter	513	609	.	+	0	ID=BBa_K143012;Name=BBa_K143012;Parent=BBa_K143053
BBa_K316020	.	promoter	575	580	.	+	0	ID=annotation1975704;Name=Sigma A-35;Parent=BBa_K143012
BBa_K316020	.	promoter	598	603	.	+	0	ID=annotation1975705;Name=Sigma A -10;Parent=BBa_K143012
BBa_K316020	.	ribosome_entry_site	618	629	.	+	0	ID=BBa_K143021;Name=BBa_K143021;Parent=BBa_K143053
BBa_K316020	.	ribosome_entry_site	618	629	.	+	0	ID=annotation1975997;Name=rbs;Parent=BBa_K143021
BBa_K316020	.	engineered_region	1552	1595	.	+	0	ID=BBa_K316014;Name=BBa_K316014
BBa_K316020	.	sequence_feature	1588	1595	.	+	0	ID=BBa_K316013;Name=BBa_K316013;Parent=BBa_K316014
BBa_K316020	.	sequence_feature	1588	1591	.	+	0	ID=annotation2098160;Name=5' product;Parent=BBa_K316013
BBa_K316020	.	sequence_feature	1592	1595	.	+	0	ID=annotation2098166;Name=3' product;Parent=BBa_K316013
BBa_K316020	.	sequence_feature	1552	1579	.	+	0	ID=BBa_K316002;Name=BBa_K316002;Parent=BBa_K316014
BBa_K316020	.	sequence_feature	1	468	.	+	0	ID=BBa_K143008;Name=BBa_K143008
BBa_K316020	.	sequence_feature	1	468	.	+	0	ID=annotation1977518;Name=PyrD 5' Integration Sequence;Parent=BBa_K143008
>BBa_K316020
atgctagaggtgaaattgccgggacttgatttgaaaaacccaatcattcctgcatcaggctgcttcggttttggaaaag
aattttcacgtttttatgatttgtcttgtcttggagctatcatgattaaggctacgacaaaggagccgcgctttgggaa
tccgacgccgcgggtagctgagactggtgctggaatgctcaatgcgatcggtctccaaaatccggggctggatagtgtg
ttgcatcatgagctgccgtggcttgagcagtttgatacaccgatcattgccaatgtcgcaggttctcaagtcgatgatt
atgttgaagtcgcagaacatatcagcaaagcgcctaatgttcatgctcttgaattgaatatttcctgcccgaatgtgaa
aacaggcggaatcgcttttggcacgaatcctgaaatggctgccgatttgacaaaagcggtgaaagaggtttcgtactag
agatctcctagaatatatattatgtaaacttactagagaattttgtcaaaataattttattgacaacgtcttattaacg
ttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgttactagagaaaggtggtgaatac
tagatgaggaggatatatttgaatacatacgaacaaattaataaagtgaaaaaaatacttcggaaacatttaaaaaata
accttattggtacttacatgtttggatcaggagttgagagtggactaaaaccaaatagtgatcttgactttttagtcgt
cgtatctgaaccattgacagatcaaagtaaagaaatacttatacaaaaaattagacctatttcaaaaaaaataggagat
aaaagcaacttacgatatattgaattaacaattattattcagcaagaaatggtaccgtggaatcatcctcccaaacaag
aatttatttatggagaatggttacaagagctttatgaacaaggatacattcctcagaaggaattaaattcagatttaac
cataatgctttaccaagcaaaacgaaaaaataaaagaatatacggaaattatgacttagaggaattactacctgatatt
ccattttctgatgtgagaagagccattatggattcgtcagaggaattaatagataattatcaggatgatgaaaccaact
ctatattaactttatgccgtatgattttaactatggacacgggtaaaatcataccaaaagatattgcgggaaatgcagt
ggctgaatcttctccattagaacatagggagagaattttgttagcagttcgtagttatcttggagagaatattgaatgg
actaatgaaaatgtaaatttaactataaactatttaaataacagattaaaaaaattataataatactagagccaggcat
caaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactaga
gtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagatctcctagaatatatattatgtaaactt
actagaggaaatttctactagaggatgtacccgtttatgtgaagctatccccgaacgtggctaatatcacagaaattgc
attagcgatcgaggaagcgggagcggacggtcttacgatgatcaacacactaatcggcatgagactcgatttaaaaacc
ggcaaaccgatattagcgaataaaacagggggactttcgggccctgctgtgaagccggttgccattcgcatggtgtatg
aagtcagccagatggtcaacatcccgattatcggaatgggaggcgtgcaaacggctgaagatgccctggaatttcttct
cgcgggagcaagcgcagtcgctgtcggaacagcaaactttgtgaatccttttgcatgtccagagattattgaacagctc
ccatctgttttgctccaatacggctatcaatcaattgaagaatgcatcggaaggagctggaatcatgaaaaacaacctg
cccatcatcgcgcttg