BBa_K316020
type: ComponentDefinition
persistent identity: BBa K316020
display id: BBa_K316020
version: 1
was generated by: iGEM to SBOL conversion
description: B. subtilis genome integration vector, targets pyrD locus
created: 2010-10-22T11:00:00Z
modified: 2015-05-08T01:11:56Z
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/Works
group u list: _440_
m user id: 0
owner id: 7480
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: IC 2010 Team
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K316014
component: BBa_K143065
component: BBa_K143053
component: BBa_K316002
component: BBa_K143009
component: BBa_K143008
sequence annotation: BBa_K143008
sequence annotation: BBa_K316002
sequence annotation: BBa_K143053
sequence annotation: BBa_K143065
sequence annotation: BBa_K316014
sequence annotation: BBa_K143009
sequence: https://synbiohub.org/public/igem/BBa_K316020_sequence/1

BBa_K316014
type: Component
persistent identity: component2098335
display id: component2098335
version: 1
top level: BBa_K316020
definition: BBa_K316014
access: public

BBa_K143065
type: Component
persistent identity: component2098330
display id: component2098330
version: 1
top level: BBa_K316020
definition: Aad9-T
access: public

BBa_K143053
type: Component
persistent identity: component2098321
display id: component2098321
version: 1
top level: BBa_K316020
definition: Pveg-spoVG
access: public

BBa_K316002
type: Component
persistent identity: component2098315
display id: component2098315
version: 1
top level: BBa_K316020
definition: Bs dif
access: public

BBa_K143009
type: Component
persistent identity: component2098337
display id: component2098337
version: 1
top level: BBa_K316020
definition: pyrD 3 IS
access: public

BBa_K143008
type: Component
persistent identity: component2098314
display id: component2098314
version: 1
top level: BBa_K316020
definition: pyrD 5 IS
access: public

BBa_K143008
type: SequenceAnnotation
persistent identity: annotation2098314
display id: annotation2098314
version: 1
top level: BBa_K316020
location: https://synbiohub.org/public/igem/BBa_K316020/annotation2098314/range2098314/1
component: BBa_K143008

https://synbiohub.org/public/igem/BBa_K316020/annotation2098314/range2098314/1
type: Range
persistent identity: range2098314
display id: range2098314
version: 1
top level: BBa_K316020
direction: direction/reverse
start: 1
end: 468
orientation: inline

BBa_K316002
type: SequenceAnnotation
persistent identity: annotation2098315
display id: annotation2098315
version: 1
top level: BBa_K316020
location: https://synbiohub.org/public/igem/BBa_K316020/annotation2098315/range2098315/1
component: BBa_K316002

https://synbiohub.org/public/igem/BBa_K316020/annotation2098315/range2098315/1
type: Range
persistent identity: range2098315
display id: range2098315
version: 1
top level: BBa_K316020
direction: direction/reverse
start: 477
end: 504
orientation: inline

BBa_K143053
type: SequenceAnnotation
persistent identity: annotation2098321
display id: annotation2098321
version: 1
top level: BBa_K316020
location: https://synbiohub.org/public/igem/BBa_K316020/annotation2098321/range2098321/1
component: BBa_K143053

https://synbiohub.org/public/igem/BBa_K316020/annotation2098321/range2098321/1
type: Range
persistent identity: range2098321
display id: range2098321
version: 1
top level: BBa_K316020
direction: direction/reverse
start: 513
end: 629
orientation: inline

BBa_K143065
type: SequenceAnnotation
persistent identity: annotation2098330
display id: annotation2098330
version: 1
top level: BBa_K316020
location: https://synbiohub.org/public/igem/BBa_K316020/annotation2098330/range2098330/1
component: BBa_K143065

https://synbiohub.org/public/igem/BBa_K316020/annotation2098330/range2098330/1
type: Range
persistent identity: range2098330
display id: range2098330
version: 1
top level: BBa_K316020
direction: direction/reverse
start: 636
end: 1543
orientation: inline

BBa_K316014
type: SequenceAnnotation
persistent identity: annotation2098335
display id: annotation2098335
version: 1
top level: BBa_K316020
location: https://synbiohub.org/public/igem/BBa_K316020/annotation2098335/range2098335/1
component: BBa_K316014

https://synbiohub.org/public/igem/BBa_K316020/annotation2098335/range2098335/1
type: Range
persistent identity: range2098335
display id: range2098335
version: 1
top level: BBa_K316020
direction: direction/reverse
start: 1552
end: 1595
orientation: inline

BBa_K143009
type: SequenceAnnotation
persistent identity: annotation2098337
display id: annotation2098337
version: 1
top level: BBa_K316020
location: https://synbiohub.org/public/igem/BBa_K316020/annotation2098337/range2098337/1
component: BBa_K143009

https://synbiohub.org/public/igem/BBa_K316020/annotation2098337/range2098337/1
type: Range
persistent identity: range2098337
display id: range2098337
version: 1
top level: BBa_K316020
direction: direction/reverse
start: 1604
end: 2070
orientation: inline

pyrD 5 IS
type: ComponentDefinition
persistent identity: BBa K143008
display id: BBa_K143008
version: 1
was generated by: iGEM to SBOL conversion
description: 5??? Integration Sequence for the pyrD locus of B. subtilis
created: 2008-09-15T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The 5??? integration sequence was taken from the B.subtilis chromosome and is homologous to the chromosome from a few hundred bp upstream of the gene's start codon until 300 bp into the gene. The part was synthesised by Geneart.
owned by: james
owned by: myers
mutable description: Integration sequences allow DNA to be incorporated into the chromosome of a host cell at a specific locus using leading (5') and trailing (3') DNA sequences that are the same as those at a specific locus of the chromosome. The 5' integration sequence can be added to the front of a Biobrick construct and the 3' integration sequence specific for this locus (<bbpart> BBa_K143004</bbpart>) to the rear of the Biobrick construct to allow integration of the Biobrick construct into the chromosome of the gram positive bacterium B.subtilis. The PyrD gene has been a target for numerous integration vectors, including the shuttle vectors pPyr-Cm (GenBank Accession number AY464558) and pPyr-Kan (GenBank Accession number AY464559) 1. Integration into the PyrD locus makes the ''B.subtilis'' auxotrophs for uracil and transformants require about 40ug/ml to allow for growth. This allows us to assay for integration by growing a replica plate with no supplemented uracil to negatively select for transformants.
discontinued: false
dominant: false
experience: experience/None
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The PyrD integration sequences were designed from the PyrD gene's Genbank entry#1 and identification of the sequence directly upstream of the gene on the chromosome (found using NCBI's sequence viewer). The upstream and PyrD gene sequence was analysed for restriction sites and primers (with biobrick prefix and suffix sequences) for two approximately equally sized integration sequences were desgined.
star: false
creator: James Chappell
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: PyrD 5' Integration Sequence
sequence: https://synbiohub.org/public/igem/BBa_K143008_sequence/1

PyrD 5' Integration Sequence
type: SequenceAnnotation
persistent identity: annotation1977518
display id: annotation1977518
version: 1
top level: pyrD 5 IS
location: https://synbiohub.org/public/igem/BBa_K143008/annotation1977518/range1977518/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K143008/annotation1977518/range1977518/1
type: Range
persistent identity: range1977518
display id: range1977518
version: 1
top level: pyrD 5 IS
direction: direction/forward
start: 1
end: 468
orientation: inline

pyrD 3 IS
type: ComponentDefinition
persistent identity: BBa K143009
display id: BBa_K143009
version: 1
was generated by: iGEM to SBOL conversion
description: 3??? Integration Sequence for the pyrD locus of B. subtilis
created: 2008-09-16T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The 3??? integration sequence was taken from the B.subtilis chromosome and is homologous to the back section of the PyrD gene. It was synthesised by Geneart.
owned by: james
owned by: myers
mutable description: Integration sequences allow DNA to be incorporated into the chromosome of a host cell at a specific locus using leading (5') and trailing (3') DNA sequences that are the same as those at a specific locus of the chromosome. The 3' integration sequence can be added to the back of a Biobrick construct and the 5' integration sequence specific for this locus (BBa_K143008) to the front of the Biobrick construct to allow integration of the Biobrick construct into the chromosome of the gram positive bacterium B.subtilis. The PyrD gene has been a target for numerous integration vectors, including the shuttle vectors pPyr-Cm (GenBank Accession number AY464558) and pPyr-Kan (GenBank Accession number AY464559) [1]. Integration into the PyrD locus makes the B.subtilis auxotrophs for uracil and transformants require about 40ug/ml to allow for growth. This allows us to assay for integration by growing a replica plate with no supplemented uracil to negatively select for transformants.
discontinued: false
dominant: false
experience: experience/None
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The PyrD integration sequences was designed from the PyrD gene's Genbank entry[1] and identification of the sequence directly upstream of the gene on the chromosome (found using NCBI's sequence viewer). The upstream and EpsE gene sequence was analysed for restriction sites and primers (with biobrick prefix and suffix sequences) for two approximately equally sized integration sequences were desgined.
star: false
creator: James Chappell
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: PyrD 3' Integration Sequence
sequence: https://synbiohub.org/public/igem/BBa_K143009_sequence/1

PyrD 3' Integration Sequence
type: SequenceAnnotation
persistent identity: annotation1977517
display id: annotation1977517
version: 1
top level: pyrD 3 IS
location: https://synbiohub.org/public/igem/BBa_K143009/annotation1977517/range1977517/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K143009/annotation1977517/range1977517/1
type: Range
persistent identity: range1977517
display id: range1977517
version: 1
top level: pyrD 3 IS
direction: direction/forward
start: 1
end: 467
orientation: inline

Pveg-spoVG
type: ComponentDefinition
persistent identity: BBa K143053
display id: BBa_K143053
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter Pveg and RBS spoVG for B. subtilis
created: 2008-10-07T11:00:00Z
modified: 2015-05-08T01:10:24Z
mutable provenance: Pveg-spoVG was synthesised by GeneArt
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Constitutive promoter veg(<bbpart>BBa_K143012</bbpart>) coupled to the strong Ribosome Binding Site spoVG(<bbpart>BBa_K143021</bbpart>) from ''B. subtilis''. Pveg-spoVG can be used in the context of a '''Ribosomes per second''' (RiPS) output generator '''To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix1. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.'''
discontinued: false
dominant: true
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The sequence of Pveg was obtained from the DBTBS1 and RBS-spoVG were obtained from papers2 and the sequence synthesised by GeneArt
star: true
creator: Chris Hirst
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K143012
component: BBa_K143021
sequence annotation: BBa_K143012
sequence annotation: BBa_K143021
sequence: https://synbiohub.org/public/igem/BBa_K143053_sequence/1

BBa_K143012
type: Component
persistent identity: component1979395
display id: component1979395
version: 1
top level: Pveg-spoVG
definition: Pveg
access: public

BBa_K143021
type: Component
persistent identity: component1979397
display id: component1979397
version: 1
top level: Pveg-spoVG
definition: RBS-spoVG
access: public

BBa_K143012
type: SequenceAnnotation
persistent identity: annotation1979395
display id: annotation1979395
version: 1
top level: Pveg-spoVG
location: https://synbiohub.org/public/igem/BBa_K143053/annotation1979395/range1979395/1
component: BBa_K143012

https://synbiohub.org/public/igem/BBa_K143053/annotation1979395/range1979395/1
type: Range
persistent identity: range1979395
display id: range1979395
version: 1
top level: Pveg-spoVG
direction: direction/reverse
start: 1
end: 97
orientation: inline

BBa_K143021
type: SequenceAnnotation
persistent identity: annotation1979397
display id: annotation1979397
version: 1
top level: Pveg-spoVG
location: https://synbiohub.org/public/igem/BBa_K143053/annotation1979397/range1979397/1
component: BBa_K143021

https://synbiohub.org/public/igem/BBa_K143053/annotation1979397/range1979397/1
type: Range
persistent identity: range1979397
display id: range1979397
version: 1
top level: Pveg-spoVG
direction: direction/reverse
start: 106
end: 117
orientation: inline

Aad9-T
type: ComponentDefinition
persistent identity: BBa K143065
display id: BBa_K143065
version: 1
was generated by: iGEM to SBOL conversion
description: Spectinomycin Resistance Protein (Aad9) - Terminator
created: 2008-10-08T11:00:00Z
modified: 2015-05-08T01:10:24Z
mutable provenance: Aad9 was PCR cloned from the ''B. subtilis'' integration vector utilising Pfu DNA polymerase and cloned into a BioBrick iwth the double terminator was taken from the registry
owned by: james
owned by: myers
mutable description: Aad9 spectinomycin resistance protein(<bbpart>BBa_K143031</bbpart>) coupled to the double terminator (<bbpart>BBa_B0015</bbpart>). Aad9 confers resistance to spectinomycin. The double terminator is the most commonly used terminator and is a combination of parts <bbpart>BBa_B0010</bbpart> and <bbpart>BBa_B0012</bbpart>. The double terminator allows the Spectinomycin resistance gene to be incorporated into a closed transcriptional unit.
discontinued: false
dominant: false
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Aad9 was PCR cloned from the ''B. subtilis'' integration vector utilising Pfu DNA polymerase. The double terminator is the most commonly used registry termiantor.
star: false
creator: Chris Hirst and Imperial iGEM 08
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K143031
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_K143031
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K143065_sequence/1

BBa_K143031
type: Component
persistent identity: component1985265
display id: component1985265
version: 1
top level: Aad9-T
definition: Aad9
access: public

BBa_B0012
type: Component
persistent identity: component1985268
display id: component1985268
version: 1
top level: Aad9-T
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1985266
display id: component1985266
version: 1
top level: Aad9-T
definition: BBa_B0010
access: public

BBa_K143031
type: SequenceAnnotation
persistent identity: annotation1985265
display id: annotation1985265
version: 1
top level: Aad9-T
location: https://synbiohub.org/public/igem/BBa_K143065/annotation1985265/range1985265/1
component: BBa_K143031

https://synbiohub.org/public/igem/BBa_K143065/annotation1985265/range1985265/1
type: Range
persistent identity: range1985265
display id: range1985265
version: 1
top level: Aad9-T
direction: direction/reverse
start: 1
end: 771
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1985266
display id: annotation1985266
version: 1
top level: Aad9-T
location: https://synbiohub.org/public/igem/BBa_K143065/annotation1985266/range1985266/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K143065/annotation1985266/range1985266/1
type: Range
persistent identity: range1985266
display id: range1985266
version: 1
top level: Aad9-T
direction: direction/forward
start: 780
end: 859
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1985268
display id: annotation1985268
version: 1
top level: Aad9-T
location: https://synbiohub.org/public/igem/BBa_K143065/annotation1985268/range1985268/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K143065/annotation1985268/range1985268/1
type: Range
persistent identity: range1985268
display id: range1985268
version: 1
top level: Aad9-T
direction: direction/forward
start: 868
end: 908
orientation: inline

Bs dif
type: ComponentDefinition
persistent identity: BBa K316002
display id: BBa_K316002
version: 1
was generated by: iGEM to SBOL conversion
description: dif excision site from B. subtilis
created: 2010-10-19T11:00:00Z
modified: 2015-05-08T01:11:56Z
mutable provenance: The dif sites were made by annealing synthestised oligoes.
owned by: james
owned by: myers
mutable description: Dif sites are naturally found in B.subtilis and are used by this organism during genome replication.
discontinued: false
dominant: false
experience: experience/None
group u list: _440_
m user id: 0
owner id: 7480
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The dif site was made by oligos designed to make overhangs for EcoRI and SpeI ( and ) or XbaI and PstI ( and ) to be used in standard Biobrick or 3A cloning.
star: false
creator: IC 2010 Team
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K316002_sequence/1

BBa_K316014
type: ComponentDefinition
persistent identity: BBa K316014
display id: BBa_K316014
version: 1
was generated by: iGEM to SBOL conversion
description: Dif sequence followed by PmeI recognition site
created: 2010-10-22T11:00:00Z
modified: 2015-05-08T01:11:56Z
mutable provenance: Oligonucleotide synthesis of single stranded primers.
owned by: james
owned by: myers
mutable description: This composite part of <bbpart>BBa_K143000</bbpart> and <bbpart>BBa_K143013</bbpart>. The dif site can be used in conjunction with another dif site in another part of the vector to remove a sequence between the two dif sites. PmeI site can be used for blunt end cloning of a DNA sequence behind the dif site. Please see ???Part Design??? section for design considerations and parts used.
discontinued: false
dominant: false
experience: experience/None
group u list: _440_
m user id: 0
owner id: 7480
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: This part was designed to be cloned using standard biobrick methods. Two single stranded, synthetic oligos were annealed to produce a double stranded DNA sequence with single stranded overhangs identical to the product of digestion by EcoRI and SpeI. Thus compatible with biobrick cloning methods.
star: false
creator: IC 2010 Team
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_K316013
component: BBa_K316002
sequence annotation: BBa_K316002
sequence annotation: BBa_K316013
sequence: https://synbiohub.org/public/igem/BBa_K316014_sequence/1

BBa_K316013
type: Component
persistent identity: component2098249
display id: component2098249
version: 1
top level: BBa_K316014
definition: PmeI site
access: public

BBa_K316002
type: Component
persistent identity: component2098246
display id: component2098246
version: 1
top level: BBa_K316014
definition: Bs dif
access: public

BBa_K316002
type: SequenceAnnotation
persistent identity: annotation2098246
display id: annotation2098246
version: 1
top level: BBa_K316014
location: https://synbiohub.org/public/igem/BBa_K316014/annotation2098246/range2098246/1
component: BBa_K316002

https://synbiohub.org/public/igem/BBa_K316014/annotation2098246/range2098246/1
type: Range
persistent identity: range2098246
display id: range2098246
version: 1
top level: BBa_K316014
direction: direction/reverse
start: 1
end: 28
orientation: inline

BBa_K316013
type: SequenceAnnotation
persistent identity: annotation2098249
display id: annotation2098249
version: 1
top level: BBa_K316014
location: https://synbiohub.org/public/igem/BBa_K316014/annotation2098249/range2098249/1
component: BBa_K316013

https://synbiohub.org/public/igem/BBa_K316014/annotation2098249/range2098249/1
type: Range
persistent identity: range2098249
display id: range2098249
version: 1
top level: BBa_K316014
direction: direction/reverse
start: 37
end: 44
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

Pveg
type: ComponentDefinition
persistent identity: BBa K143012
display id: BBa_K143012
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter veg a constitutive promoter for B. subtilis
created: 2008-09-10T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The Pveg promoter was suggested to us by Dr. Jan-Willem Veening of Newcastle University. This sequence supplied was compared to that of the DBTBS database#3 then a section containing the binding site synthesised by Geneart.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Pveg is a constitutive promoter that constitutively expresses the P43 protein in ''B.subtilis''. Pveg contains binding sites for the ''B.sutbilis'' major sigma factor#1. Pveg in ''B.subtilis'' utilises two binding sites to cause high expression of genes#2, however our Pveg is lacking the upstream site to give a medium level of gene expression. It has been noted that the sporulation master regulatoion factor spoOA interacts with Pveg though it is not known how#3. The context with which we used the promoter Pveg is as a '''Polymerase Per Second''' (PoPS) generator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The biobrick part was designed to include a single binding site for the ''B.subtilis major sigma factor. In addition the biobrick standard was applied to the promoter Pveg sequence.
star: false
creator: James Chappell
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: Sigma A-35
sequence annotation: Sigma A -10
sequence: https://synbiohub.org/public/igem/BBa_K143012_sequence/1

Sigma A-35
type: SequenceAnnotation
persistent identity: annotation1975704
display id: annotation1975704
version: 1
top level: Pveg
location: https://synbiohub.org/public/igem/BBa_K143012/annotation1975704/range1975704/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K143012/annotation1975704/range1975704/1
type: Range
persistent identity: range1975704
display id: range1975704
version: 1
top level: Pveg
direction: direction/forward
start: 63
end: 68
orientation: inline

Sigma A -10
type: SequenceAnnotation
persistent identity: annotation1975705
display id: annotation1975705
version: 1
top level: Pveg
location: https://synbiohub.org/public/igem/BBa_K143012/annotation1975705/range1975705/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K143012/annotation1975705/range1975705/1
type: Range
persistent identity: range1975705
display id: range1975705
version: 1
top level: Pveg
direction: direction/forward
start: 86
end: 91
orientation: inline

Aad9
type: ComponentDefinition
persistent identity: BBa K143031
display id: BBa_K143031
version: 1
was generated by: iGEM to SBOL conversion
description: Aad9 Spectinomycin Resistance Gene
created: 2008-09-15T11:00:00Z
modified: 2015-05-08T01:10:24Z
mutable provenance: Aad9 was PCR cloned from the ''B. subtilis'' integration vector pDR111 using Pfu DNA polymerase
owned by: james
owned by: myers
mutable description: Aad9 is the spectinomycin resistance gene from ''Enterococcus faecalis''#1. Expression in a host confers resistance to spectinomycin at a concentration of 100μg/μl and has been used in a variety of vectors for both ''B. subtilis'' and ''E. coli'' including pDP870#2, pCOMT-Kan#3 and pIEF16s#4 ====References==== <biblio> #1 pmid=1659306 #2 pmid=16997985 #3 pmid=15060042 #4 pmid=16714443 </biblio>
discontinued: false
dominant: false
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The sequence of ''B. subtilis'' integration vector pDR111 was searched for the spectinomycin resistance gene and the Biobrick standard applied to the gene sequence upon PCR cloning
star: false
creator: Chris Hirst
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Aad9 Spectinomycin Acetyltransferase
sequence annotation: start
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K143031_sequence/1

Aad9 Spectinomycin Acetyltransferase
type: SequenceAnnotation
persistent identity: annotation1975961
display id: annotation1975961
version: 1
top level: Aad9
location: https://synbiohub.org/public/igem/BBa_K143031/annotation1975961/range1975961/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K143031/annotation1975961/range1975961/1
type: Range
persistent identity: range1975961
display id: range1975961
version: 1
top level: Aad9
direction: direction/forward
start: 1
end: 765
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1992698
display id: annotation1992698
version: 1
top level: Aad9
location: https://synbiohub.org/public/igem/BBa_K143031/annotation1992698/range1992698/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K143031/annotation1992698/range1992698/1
type: Range
persistent identity: range1992698
display id: range1992698
version: 1
top level: Aad9
direction: direction/forward
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1992696
display id: annotation1992696
version: 1
top level: Aad9
location: https://synbiohub.org/public/igem/BBa_K143031/annotation1992696/range1992696/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K143031/annotation1992696/range1992696/1
type: Range
persistent identity: range1992696
display id: range1992696
version: 1
top level: Aad9
direction: direction/forward
start: 766
end: 768
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1992697
display id: annotation1992697
version: 1
top level: Aad9
location: https://synbiohub.org/public/igem/BBa_K143031/annotation1992697/range1992697/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K143031/annotation1992697/range1992697/1
type: Range
persistent identity: range1992697
display id: range1992697
version: 1
top level: Aad9
direction: direction/forward
start: 769
end: 771
orientation: inline

RBS-spoVG
type: ComponentDefinition
persistent identity: BBa K143021
display id: BBa_K143021
version: 1
was generated by: iGEM to SBOL conversion
description: SpoVG ribosome binding site (RBS) for B. subtilis
created: 2008-09-16T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The sequence was taken from a previous research paper [1] and was constructed by Geneart.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Description: SpoVG is an endogenous ribosome binding site from B.subtilis. The sequence of the spoVG ribosome binding site is AAAGGUGGUGA which is complementary to the sequence UUUCCUCCACU from the 3' region of the 16s rRNA from B.subtilis. Previous research showed that the predicted binding energy of the 16s rRNA to the RBS is -19kcal 1
discontinued: false
dominant: true
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In order to ensure that the RBS is functional the actual ribosome binding site was maintained and the distance between the RBS and the start codon maintained. In order to conform to the biobrick standard the sequence flanking the RBS had to be changed but the distance between the promoter and RBS, and start codon and RBS was maintained.
star: false
creator: James Chappell
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K143021_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1975997
display id: annotation1975997
version: 1
top level: RBS-spoVG
location: https://synbiohub.org/public/igem/BBa_K143021/annotation1975997/range1975997/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K143021/annotation1975997/range1975997/1
type: Range
persistent identity: range1975997
display id: range1975997
version: 1
top level: RBS-spoVG
direction: direction/forward
start: 1
end: 12
orientation: inline

PmeI site
type: ComponentDefinition
persistent identity: BBa K316013
display id: BBa_K316013
version: 1
was generated by: iGEM to SBOL conversion
description: 8bp recognition sequence for PmeI restriction endonuclease
created: 2010-10-22T11:00:00Z
modified: 2015-05-08T01:11:56Z
mutable provenance: This is a planning part
owned by: james
owned by: myers
mutable description: Information about PmeI restriction endonuclease is available at [http://www.neb.com/nebecomm/products/productR0560.asp]. The recognition site is a 8bp sequence GTTTAAAC. Pme produces a blunt cut after GTTT.
discontinued: false
dominant: false
experience: experience/None
group u list: _440_
m user id: 0
owner id: 7480
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This is a planning part
star: false
creator: IC 2010 Team
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: 5' product
sequence annotation: 3' product
sequence: https://synbiohub.org/public/igem/BBa_K316013_sequence/1

5' product
type: SequenceAnnotation
persistent identity: annotation2098160
display id: annotation2098160
version: 1
top level: PmeI site
location: https://synbiohub.org/public/igem/BBa_K316013/annotation2098160/range2098160/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K316013/annotation2098160/range2098160/1
type: Range
persistent identity: range2098160
display id: range2098160
version: 1
top level: PmeI site
direction: direction/forward
start: 1
end: 4
orientation: inline

3' product
type: SequenceAnnotation
persistent identity: annotation2098166
display id: annotation2098166
version: 1
top level: PmeI site
location: https://synbiohub.org/public/igem/BBa_K316013/annotation2098166/range2098166/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K316013/annotation2098166/range2098166/1
type: Range
persistent identity: range2098166
display id: range2098166
version: 1
top level: PmeI site
direction: direction/forward
start: 5
end: 8
orientation: inline

https://synbiohub.org/public/igem/BBa_K316020_sequence/1
type: Sequence
persistent identity: BBa K316020 sequence
display id: BBa_K316020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgctagaggtgaaattgccgggacttgatttgaaaaacccaatcattcctgcatcaggctgcttcggttttggaaaagaattttcacgtttttatgatttgtcttgtcttggagctatcatgattaaggctacgacaaaggagccgcgctttgggaatccgacgccgcgggtagctgagactggtgctggaatgctcaatgcgatcggtctccaaaatccggggctggatagtgtgttgcatcatgagctgccgtggcttgagcagtttgatacaccgatcattgccaatgtcgcaggttctcaagtcgatgattatgttgaagtcgcagaacatatcagcaaagcgcctaatgttcatgctcttgaattgaatatttcctgcccgaatgtgaaaacaggcggaatcgcttttggcacgaatcctgaaatggctgccgatttgacaaaagcggtgaaagaggtttcgtactagagatctcctagaatatatattatgtaaacttactagagaattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgttactagagaaaggtggtgaatactagatgaggaggatatatttgaatacatacgaacaaattaataaagtgaaaaaaatacttcggaaacatttaaaaaataaccttattggtacttacatgtttggatcaggagttgagagtggactaaaaccaaatagtgatcttgactttttagtcgtcgtatctgaaccattgacagatcaaagtaaagaaatacttatacaaaaaattagacctatttcaaaaaaaataggagataaaagcaacttacgatatattgaattaacaattattattcagcaagaaatggtaccgtggaatcatcctcccaaacaagaatttatttatggagaatggttacaagagctttatgaacaaggatacattcctcagaaggaattaaattcagatttaaccataatgctttaccaagcaaaacgaaaaaataaaagaatatacggaaattatgacttagaggaattactacctgatattccattttctgatgtgagaagagccattatggattcgtcagaggaattaatagataattatcaggatgatgaaaccaactctatattaactttatgccgtatgattttaactatggacacgggtaaaatcataccaaaagatattgcgggaaatgcagtggctgaatcttctccattagaacatagggagagaattttgttagcagttcgtagttatcttggagagaatattgaatggactaatgaaaatgtaaatttaactataaactatttaaataacagattaaaaaaattataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagatctcctagaatatatattatgtaaacttactagaggaaatttctactagaggatgtacccgtttatgtgaagctatccccgaacgtggctaatatcacagaaattgcattagcgatcgaggaagcgggagcggacggtcttacgatgatcaacacactaatcggcatgagactcgatttaaaaaccggcaaaccgatattagcgaataaaacagggggactttcgggccctgctgtgaagccggttgccattcgcatggtgtatgaagtcagccagatggtcaacatcccgattatcggaatgggaggcgtgcaaacggctgaagatgccctggaatttcttctcgcgggagcaagcgcagtcgctgtcggaacagcaaactttgtgaatccttttgcatgtccagagattattgaacagctcccatctgttttgctccaatacggct…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143008_sequence/1
type: Sequence
persistent identity: BBa K143008 sequence
display id: BBa_K143008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgctagaggtgaaattgccgggacttgatttgaaaaacccaatcattcctgcatcaggctgcttcggttttggaaaagaattttcacgtttttatgatttgtcttgtcttggagctatcatgattaaggctacgacaaaggagccgcgctttgggaatccgacgccgcgggtagctgagactggtgctggaatgctcaatgcgatcggtctccaaaatccggggctggatagtgtgttgcatcatgagctgccgtggcttgagcagtttgatacaccgatcattgccaatgtcgcaggttctcaagtcgatgattatgttgaagtcgcagaacatatcagcaaagcgcctaatgttcatgctcttgaattgaatatttcctgcccgaatgtgaaaacaggcggaatcgcttttggcacgaatcctgaaatggctgccgatttgacaaaagcggtgaaagaggtttcg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143009_sequence/1
type: Sequence
persistent identity: BBa K143009 sequence
display id: BBa_K143009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gatgtacccgtttatgtgaagctatccccgaacgtggctaatatcacagaaattgcattagcgatcgaggaagcgggagcggacggtcttacgatgatcaacacactaatcggcatgagactcgatttaaaaaccggcaaaccgatattagcgaataaaacagggggactttcgggccctgctgtgaagccggttgccattcgcatggtgtatgaagtcagccagatggtcaacatcccgattatcggaatgggaggcgtgcaaacggctgaagatgccctggaatttcttctcgcgggagcaagcgcagtcgctgtcggaacagcaaactttgtgaatccttttgcatgtccagagattattgaacagctcccatctgttttgctccaatacggctatcaatcaattgaagaatgcatcggaaggagctggaatcatgaaaaacaacctgcccatcatcgcgcttg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143053_sequence/1
type: Sequence
persistent identity: BBa K143053 sequence
display id: BBa_K143053_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgttactagagaaaggtggtgaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143065_sequence/1
type: Sequence
persistent identity: BBa K143065 sequence
display id: BBa_K143065_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaggaggatatatttgaatacatacgaacaaattaataaagtgaaaaaaatacttcggaaacatttaaaaaataaccttattggtacttacatgtttggatcaggagttgagagtggactaaaaccaaatagtgatcttgactttttagtcgtcgtatctgaaccattgacagatcaaagtaaagaaatacttatacaaaaaattagacctatttcaaaaaaaataggagataaaagcaacttacgatatattgaattaacaattattattcagcaagaaatggtaccgtggaatcatcctcccaaacaagaatttatttatggagaatggttacaagagctttatgaacaaggatacattcctcagaaggaattaaattcagatttaaccataatgctttaccaagcaaaacgaaaaaataaaagaatatacggaaattatgacttagaggaattactacctgatattccattttctgatgtgagaagagccattatggattcgtcagaggaattaatagataattatcaggatgatgaaaccaactctatattaactttatgccgtatgattttaactatggacacgggtaaaatcataccaaaagatattgcgggaaatgcagtggctgaatcttctccattagaacatagggagagaattttgttagcagttcgtagttatcttggagagaatattgaatggactaatgaaaatgtaaatttaactataaactatttaaataacagattaaaaaaattataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K316002_sequence/1
type: Sequence
persistent identity: BBa K316002 sequence
display id: BBa_K316002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atctcctagaatatatattatgtaaact
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K316014_sequence/1
type: Sequence
persistent identity: BBa K316014 sequence
display id: BBa_K316014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atctcctagaatatatattatgtaaacttactagaggaaatttc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143012_sequence/1
type: Sequence
persistent identity: BBa K143012 sequence
display id: BBa_K143012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143031_sequence/1
type: Sequence
persistent identity: BBa K143031 sequence
display id: BBa_K143031_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaggaggatatatttgaatacatacgaacaaattaataaagtgaaaaaaatacttcggaaacatttaaaaaataaccttattggtacttacatgtttggatcaggagttgagagtggactaaaaccaaatagtgatcttgactttttagtcgtcgtatctgaaccattgacagatcaaagtaaagaaatacttatacaaaaaattagacctatttcaaaaaaaataggagataaaagcaacttacgatatattgaattaacaattattattcagcaagaaatggtaccgtggaatcatcctcccaaacaagaatttatttatggagaatggttacaagagctttatgaacaaggatacattcctcagaaggaattaaattcagatttaaccataatgctttaccaagcaaaacgaaaaaataaaagaatatacggaaattatgacttagaggaattactacctgatattccattttctgatgtgagaagagccattatggattcgtcagaggaattaatagataattatcaggatgatgaaaccaactctatattaactttatgccgtatgattttaactatggacacgggtaaaatcataccaaaagatattgcgggaaatgcagtggctgaatcttctccattagaacatagggagagaattttgttagcagttcgtagttatcttggagagaatattgaatggactaatgaaaatgtaaatttaactataaactatttaaataacagattaaaaaaattataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143021_sequence/1
type: Sequence
persistent identity: BBa K143021 sequence
display id: BBa_K143021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaggtggtgaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K316013_sequence/1
type: Sequence
persistent identity: BBa K316013 sequence
display id: BBa_K316013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaaatttc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z