LOCUS       BBa_K316021             1031 bp    DNA     linear       25-Nov-2024
DEFINITION  Spectinomycin resistance with promoter for B.subtilis
ACCESSION   BBa_K316021
VERSION     BBa_K316021.1  
FEATURES             Location/Qualifiers
     promoter        63..68
                     /label=Sigma A-35
     promoter        86..91
                     /label=Sigma A -10
     promoter        1..97
                     /label=BBa_K143012
     RBS             106..117
                     /label=rbs
     RBS             106..117
                     /label=BBa_K143021
     misc_feature    1..117
                     /label=BBa_K143053
     CDS             124..888
                     /label=Aad9 Spectinomycin Acetyltransferase
     misc_feature    889..891
                     /label=stop
     misc_feature    892..894
                     /label=stop
     misc_feature    124..126
                     /label=start
     CDS             124..894
                     /label=BBa_K143031
     stem_loop       914..957
                     /label=stem_loop
     misc_feature    903..982
                     /label=BBa_B0010
     terminator      903..982
                     /label=BBa_B0010
     stem_loop       998..1017
                     /label=T7 TE
     misc_feature    1024..1024
                     /label=stop
     polyA_site      1018..1031
                     /label=polya
     misc_feature    991..1031
                     /label=BBa_B0012
     terminator      991..1031
                     /label=BBa_B0012
     misc_feature    124..1031
                     /label=BBa_K143065
ORIGIN
        1 aattttgtca aaataatttt attgacaacg tcttattaac gttgatataa tttaaatttt
       61 atttgacaaa aatgggctcg tgttgtacaa taaatgttac tagagaaagg tggtgaatac
      121 tagatgagga ggatatattt gaatacatac gaacaaatta ataaagtgaa aaaaatactt
      181 cggaaacatt taaaaaataa ccttattggt acttacatgt ttggatcagg agttgagagt
      241 ggactaaaac caaatagtga tcttgacttt ttagtcgtcg tatctgaacc attgacagat
      301 caaagtaaag aaatacttat acaaaaaatt agacctattt caaaaaaaat aggagataaa
      361 agcaacttac gatatattga attaacaatt attattcagc aagaaatggt accgtggaat
      421 catcctccca aacaagaatt tatttatgga gaatggttac aagagcttta tgaacaagga
      481 tacattcctc agaaggaatt aaattcagat ttaaccataa tgctttacca agcaaaacga
      541 aaaaataaaa gaatatacgg aaattatgac ttagaggaat tactacctga tattccattt
      601 tctgatgtga gaagagccat tatggattcg tcagaggaat taatagataa ttatcaggat
      661 gatgaaacca actctatatt aactttatgc cgtatgattt taactatgga cacgggtaaa
      721 atcataccaa aagatattgc gggaaatgca gtggctgaat cttctccatt agaacatagg
      781 gagagaattt tgttagcagt tcgtagttat cttggagaga atattgaatg gactaatgaa
      841 aatgtaaatt taactataaa ctatttaaat aacagattaa aaaaattata ataatactag
      901 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
      961 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     1021 ctgcgtttat a
//