##gff-version 3
##sequence-region BBa_K316021 1 1031
BBa_K316021	.	engineered_region	1	117	.	+	0	ID=BBa_K143053;Name=BBa_K143053
BBa_K316021	.	ribosome_entry_site	106	117	.	+	0	ID=BBa_K143021;Name=BBa_K143021;Parent=BBa_K143053
BBa_K316021	.	ribosome_entry_site	106	117	.	+	0	ID=annotation1975997;Name=rbs;Parent=BBa_K143021
BBa_K316021	.	promoter	1	97	.	+	0	ID=BBa_K143012;Name=BBa_K143012;Parent=BBa_K143053
BBa_K316021	.	promoter	63	68	.	+	0	ID=annotation1975704;Name=Sigma A-35;Parent=BBa_K143012
BBa_K316021	.	promoter	86	91	.	+	0	ID=annotation1975705;Name=Sigma A -10;Parent=BBa_K143012
BBa_K316021	.	engineered_region	124	1031	.	+	0	ID=BBa_K143065;Name=BBa_K143065
BBa_K316021	.	terminator	903	982	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K143065
BBa_K316021	.	stem_loop	914	957	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K316021	.	engineered_region	903	982	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K316021	.	CDS	124	894	.	+	0	ID=BBa_K143031;Name=BBa_K143031;Parent=BBa_K143065
BBa_K316021	.	start_codon	124	126	.	+	0	ID=annotation1992698;Name=start;Parent=BBa_K143031
BBa_K316021	.	CDS	124	888	.	+	0	ID=annotation1975961;Name=Aad9 Spectinomycin Acetyltransferase;Parent=BBa_K143031
BBa_K316021	.	stop_codon	889	891	.	+	0	ID=annotation1992696;Name=stop;Parent=BBa_K143031
BBa_K316021	.	stop_codon	892	894	.	+	0	ID=annotation1992697;Name=stop;Parent=BBa_K143031
BBa_K316021	.	terminator	991	1031	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K143065
BBa_K316021	.	polyA_site	1018	1031	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K316021	.	engineered_region	991	1031	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K316021	.	stop_codon	1024	1024	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K316021	.	stem_loop	998	1017	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
>BBa_K316021
aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctc
gtgttgtacaataaatgttactagagaaaggtggtgaatactagatgaggaggatatatttgaatacatacgaacaaat
taataaagtgaaaaaaatacttcggaaacatttaaaaaataaccttattggtacttacatgtttggatcaggagttgag
agtggactaaaaccaaatagtgatcttgactttttagtcgtcgtatctgaaccattgacagatcaaagtaaagaaatac
ttatacaaaaaattagacctatttcaaaaaaaataggagataaaagcaacttacgatatattgaattaacaattattat
tcagcaagaaatggtaccgtggaatcatcctcccaaacaagaatttatttatggagaatggttacaagagctttatgaa
caaggatacattcctcagaaggaattaaattcagatttaaccataatgctttaccaagcaaaacgaaaaaataaaagaa
tatacggaaattatgacttagaggaattactacctgatattccattttctgatgtgagaagagccattatggattcgtc
agaggaattaatagataattatcaggatgatgaaaccaactctatattaactttatgccgtatgattttaactatggac
acgggtaaaatcataccaaaagatattgcgggaaatgcagtggctgaatcttctccattagaacatagggagagaattt
tgttagcagttcgtagttatcttggagagaatattgaatggactaatgaaaatgtaaatttaactataaactatttaaa
taacagattaaaaaaattataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctt
tcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtt
tata