BBa_K316021
type: ComponentDefinition
persistent identity: BBa K316021
display id: BBa_K316021
version: 1
was generated by: iGEM to SBOL conversion
description: Spectinomycin resistance with promoter for B.subtilis
created: 2010-10-22T11:00:00Z
modified: 2015-05-08T01:11:56Z
mutable provenance: Existing biobricks <bbpart>BBa_K143053</bbpart>, <bbpart>BBa_K143065</bbpart>
owned by: james
owned by: myers
mutable description: For reliable expression in B. subtilis the strongest constitutive promoter Pveg and strong ribosome binding site spoVG <bbpart>BBa_K143053</bbpart> were combined with spectinomycin resistance gene with terminator <bbpart>BBa_K143065</bbpart> Please see ???Part Design??? section for design considerations and parts used.
discontinued: false
dominant: false
experience: experience/Works
group u list: _440_
m user id: 0
owner id: 7480
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Standard biobrick assembly [http://partsregistry.org/Assembly:Standard_assembly]
star: false
creator: IC 2010 Team
type: DnaRegion
role: SO:0000316
role: partType/Coding
component: BBa_K143065
component: BBa_K143053
sequence annotation: BBa_K143053
sequence annotation: BBa_K143065
sequence: https://synbiohub.org/public/igem/BBa_K316021_sequence/1

BBa_K143065
type: Component
persistent identity: component2093436
display id: component2093436
version: 1
top level: BBa_K316021
definition: Aad9-T
access: public

BBa_K143053
type: Component
persistent identity: component2093427
display id: component2093427
version: 1
top level: BBa_K316021
definition: Pveg-spoVG
access: public

BBa_K143053
type: SequenceAnnotation
persistent identity: annotation2093427
display id: annotation2093427
version: 1
top level: BBa_K316021
location: https://synbiohub.org/public/igem/BBa_K316021/annotation2093427/range2093427/1
component: BBa_K143053

https://synbiohub.org/public/igem/BBa_K316021/annotation2093427/range2093427/1
type: Range
persistent identity: range2093427
display id: range2093427
version: 1
top level: BBa_K316021
direction: direction/reverse
start: 1
end: 117
orientation: inline

BBa_K143065
type: SequenceAnnotation
persistent identity: annotation2093436
display id: annotation2093436
version: 1
top level: BBa_K316021
location: https://synbiohub.org/public/igem/BBa_K316021/annotation2093436/range2093436/1
component: BBa_K143065

https://synbiohub.org/public/igem/BBa_K316021/annotation2093436/range2093436/1
type: Range
persistent identity: range2093436
display id: range2093436
version: 1
top level: BBa_K316021
direction: direction/reverse
start: 124
end: 1031
orientation: inline

Pveg-spoVG
type: ComponentDefinition
persistent identity: BBa K143053
display id: BBa_K143053
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter Pveg and RBS spoVG for B. subtilis
created: 2008-10-07T11:00:00Z
modified: 2015-05-08T01:10:24Z
mutable provenance: Pveg-spoVG was synthesised by GeneArt
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Constitutive promoter veg(<bbpart>BBa_K143012</bbpart>) coupled to the strong Ribosome Binding Site spoVG(<bbpart>BBa_K143021</bbpart>) from ''B. subtilis''. Pveg-spoVG can be used in the context of a '''Ribosomes per second''' (RiPS) output generator '''To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix1. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.'''
discontinued: false
dominant: true
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The sequence of Pveg was obtained from the DBTBS1 and RBS-spoVG were obtained from papers2 and the sequence synthesised by GeneArt
star: true
creator: Chris Hirst
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K143012
component: BBa_K143021
sequence annotation: BBa_K143012
sequence annotation: BBa_K143021
sequence: https://synbiohub.org/public/igem/BBa_K143053_sequence/1

BBa_K143012
type: Component
persistent identity: component1979395
display id: component1979395
version: 1
top level: Pveg-spoVG
definition: Pveg
access: public

BBa_K143021
type: Component
persistent identity: component1979397
display id: component1979397
version: 1
top level: Pveg-spoVG
definition: RBS-spoVG
access: public

BBa_K143012
type: SequenceAnnotation
persistent identity: annotation1979395
display id: annotation1979395
version: 1
top level: Pveg-spoVG
location: https://synbiohub.org/public/igem/BBa_K143053/annotation1979395/range1979395/1
component: BBa_K143012

https://synbiohub.org/public/igem/BBa_K143053/annotation1979395/range1979395/1
type: Range
persistent identity: range1979395
display id: range1979395
version: 1
top level: Pveg-spoVG
direction: direction/reverse
start: 1
end: 97
orientation: inline

BBa_K143021
type: SequenceAnnotation
persistent identity: annotation1979397
display id: annotation1979397
version: 1
top level: Pveg-spoVG
location: https://synbiohub.org/public/igem/BBa_K143053/annotation1979397/range1979397/1
component: BBa_K143021

https://synbiohub.org/public/igem/BBa_K143053/annotation1979397/range1979397/1
type: Range
persistent identity: range1979397
display id: range1979397
version: 1
top level: Pveg-spoVG
direction: direction/reverse
start: 106
end: 117
orientation: inline

Aad9-T
type: ComponentDefinition
persistent identity: BBa K143065
display id: BBa_K143065
version: 1
was generated by: iGEM to SBOL conversion
description: Spectinomycin Resistance Protein (Aad9) - Terminator
created: 2008-10-08T11:00:00Z
modified: 2015-05-08T01:10:24Z
mutable provenance: Aad9 was PCR cloned from the ''B. subtilis'' integration vector utilising Pfu DNA polymerase and cloned into a BioBrick iwth the double terminator was taken from the registry
owned by: james
owned by: myers
mutable description: Aad9 spectinomycin resistance protein(<bbpart>BBa_K143031</bbpart>) coupled to the double terminator (<bbpart>BBa_B0015</bbpart>). Aad9 confers resistance to spectinomycin. The double terminator is the most commonly used terminator and is a combination of parts <bbpart>BBa_B0010</bbpart> and <bbpart>BBa_B0012</bbpart>. The double terminator allows the Spectinomycin resistance gene to be incorporated into a closed transcriptional unit.
discontinued: false
dominant: false
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Aad9 was PCR cloned from the ''B. subtilis'' integration vector utilising Pfu DNA polymerase. The double terminator is the most commonly used registry termiantor.
star: false
creator: Chris Hirst and Imperial iGEM 08
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K143031
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_K143031
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K143065_sequence/1

BBa_K143031
type: Component
persistent identity: component1985265
display id: component1985265
version: 1
top level: Aad9-T
definition: Aad9
access: public

BBa_B0012
type: Component
persistent identity: component1985268
display id: component1985268
version: 1
top level: Aad9-T
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1985266
display id: component1985266
version: 1
top level: Aad9-T
definition: BBa_B0010
access: public

BBa_K143031
type: SequenceAnnotation
persistent identity: annotation1985265
display id: annotation1985265
version: 1
top level: Aad9-T
location: https://synbiohub.org/public/igem/BBa_K143065/annotation1985265/range1985265/1
component: BBa_K143031

https://synbiohub.org/public/igem/BBa_K143065/annotation1985265/range1985265/1
type: Range
persistent identity: range1985265
display id: range1985265
version: 1
top level: Aad9-T
direction: direction/reverse
start: 1
end: 771
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1985266
display id: annotation1985266
version: 1
top level: Aad9-T
location: https://synbiohub.org/public/igem/BBa_K143065/annotation1985266/range1985266/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K143065/annotation1985266/range1985266/1
type: Range
persistent identity: range1985266
display id: range1985266
version: 1
top level: Aad9-T
direction: direction/forward
start: 780
end: 859
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1985268
display id: annotation1985268
version: 1
top level: Aad9-T
location: https://synbiohub.org/public/igem/BBa_K143065/annotation1985268/range1985268/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K143065/annotation1985268/range1985268/1
type: Range
persistent identity: range1985268
display id: range1985268
version: 1
top level: Aad9-T
direction: direction/forward
start: 868
end: 908
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

Pveg
type: ComponentDefinition
persistent identity: BBa K143012
display id: BBa_K143012
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter veg a constitutive promoter for B. subtilis
created: 2008-09-10T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The Pveg promoter was suggested to us by Dr. Jan-Willem Veening of Newcastle University. This sequence supplied was compared to that of the DBTBS database#3 then a section containing the binding site synthesised by Geneart.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Pveg is a constitutive promoter that constitutively expresses the P43 protein in ''B.subtilis''. Pveg contains binding sites for the ''B.sutbilis'' major sigma factor#1. Pveg in ''B.subtilis'' utilises two binding sites to cause high expression of genes#2, however our Pveg is lacking the upstream site to give a medium level of gene expression. It has been noted that the sporulation master regulatoion factor spoOA interacts with Pveg though it is not known how#3. The context with which we used the promoter Pveg is as a '''Polymerase Per Second''' (PoPS) generator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The biobrick part was designed to include a single binding site for the ''B.subtilis major sigma factor. In addition the biobrick standard was applied to the promoter Pveg sequence.
star: false
creator: James Chappell
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: Sigma A-35
sequence annotation: Sigma A -10
sequence: https://synbiohub.org/public/igem/BBa_K143012_sequence/1

Sigma A-35
type: SequenceAnnotation
persistent identity: annotation1975704
display id: annotation1975704
version: 1
top level: Pveg
location: https://synbiohub.org/public/igem/BBa_K143012/annotation1975704/range1975704/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K143012/annotation1975704/range1975704/1
type: Range
persistent identity: range1975704
display id: range1975704
version: 1
top level: Pveg
direction: direction/forward
start: 63
end: 68
orientation: inline

Sigma A -10
type: SequenceAnnotation
persistent identity: annotation1975705
display id: annotation1975705
version: 1
top level: Pveg
location: https://synbiohub.org/public/igem/BBa_K143012/annotation1975705/range1975705/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K143012/annotation1975705/range1975705/1
type: Range
persistent identity: range1975705
display id: range1975705
version: 1
top level: Pveg
direction: direction/forward
start: 86
end: 91
orientation: inline

Aad9
type: ComponentDefinition
persistent identity: BBa K143031
display id: BBa_K143031
version: 1
was generated by: iGEM to SBOL conversion
description: Aad9 Spectinomycin Resistance Gene
created: 2008-09-15T11:00:00Z
modified: 2015-05-08T01:10:24Z
mutable provenance: Aad9 was PCR cloned from the ''B. subtilis'' integration vector pDR111 using Pfu DNA polymerase
owned by: james
owned by: myers
mutable description: Aad9 is the spectinomycin resistance gene from ''Enterococcus faecalis''#1. Expression in a host confers resistance to spectinomycin at a concentration of 100μg/μl and has been used in a variety of vectors for both ''B. subtilis'' and ''E. coli'' including pDP870#2, pCOMT-Kan#3 and pIEF16s#4 ====References==== <biblio> #1 pmid=1659306 #2 pmid=16997985 #3 pmid=15060042 #4 pmid=16714443 </biblio>
discontinued: false
dominant: false
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The sequence of ''B. subtilis'' integration vector pDR111 was searched for the spectinomycin resistance gene and the Biobrick standard applied to the gene sequence upon PCR cloning
star: false
creator: Chris Hirst
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Aad9 Spectinomycin Acetyltransferase
sequence annotation: start
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K143031_sequence/1

Aad9 Spectinomycin Acetyltransferase
type: SequenceAnnotation
persistent identity: annotation1975961
display id: annotation1975961
version: 1
top level: Aad9
location: https://synbiohub.org/public/igem/BBa_K143031/annotation1975961/range1975961/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K143031/annotation1975961/range1975961/1
type: Range
persistent identity: range1975961
display id: range1975961
version: 1
top level: Aad9
direction: direction/forward
start: 1
end: 765
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1992698
display id: annotation1992698
version: 1
top level: Aad9
location: https://synbiohub.org/public/igem/BBa_K143031/annotation1992698/range1992698/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K143031/annotation1992698/range1992698/1
type: Range
persistent identity: range1992698
display id: range1992698
version: 1
top level: Aad9
direction: direction/forward
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1992696
display id: annotation1992696
version: 1
top level: Aad9
location: https://synbiohub.org/public/igem/BBa_K143031/annotation1992696/range1992696/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K143031/annotation1992696/range1992696/1
type: Range
persistent identity: range1992696
display id: range1992696
version: 1
top level: Aad9
direction: direction/forward
start: 766
end: 768
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1992697
display id: annotation1992697
version: 1
top level: Aad9
location: https://synbiohub.org/public/igem/BBa_K143031/annotation1992697/range1992697/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K143031/annotation1992697/range1992697/1
type: Range
persistent identity: range1992697
display id: range1992697
version: 1
top level: Aad9
direction: direction/forward
start: 769
end: 771
orientation: inline

RBS-spoVG
type: ComponentDefinition
persistent identity: BBa K143021
display id: BBa_K143021
version: 1
was generated by: iGEM to SBOL conversion
description: SpoVG ribosome binding site (RBS) for B. subtilis
created: 2008-09-16T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The sequence was taken from a previous research paper [1] and was constructed by Geneart.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Description: SpoVG is an endogenous ribosome binding site from B.subtilis. The sequence of the spoVG ribosome binding site is AAAGGUGGUGA which is complementary to the sequence UUUCCUCCACU from the 3' region of the 16s rRNA from B.subtilis. Previous research showed that the predicted binding energy of the 16s rRNA to the RBS is -19kcal 1
discontinued: false
dominant: true
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In order to ensure that the RBS is functional the actual ribosome binding site was maintained and the distance between the RBS and the start codon maintained. In order to conform to the biobrick standard the sequence flanking the RBS had to be changed but the distance between the promoter and RBS, and start codon and RBS was maintained.
star: false
creator: James Chappell
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K143021_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1975997
display id: annotation1975997
version: 1
top level: RBS-spoVG
location: https://synbiohub.org/public/igem/BBa_K143021/annotation1975997/range1975997/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K143021/annotation1975997/range1975997/1
type: Range
persistent identity: range1975997
display id: range1975997
version: 1
top level: RBS-spoVG
direction: direction/forward
start: 1
end: 12
orientation: inline

https://synbiohub.org/public/igem/BBa_K316021_sequence/1
type: Sequence
persistent identity: BBa K316021 sequence
display id: BBa_K316021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgttactagagaaaggtggtgaatactagatgaggaggatatatttgaatacatacgaacaaattaataaagtgaaaaaaatacttcggaaacatttaaaaaataaccttattggtacttacatgtttggatcaggagttgagagtggactaaaaccaaatagtgatcttgactttttagtcgtcgtatctgaaccattgacagatcaaagtaaagaaatacttatacaaaaaattagacctatttcaaaaaaaataggagataaaagcaacttacgatatattgaattaacaattattattcagcaagaaatggtaccgtggaatcatcctcccaaacaagaatttatttatggagaatggttacaagagctttatgaacaaggatacattcctcagaaggaattaaattcagatttaaccataatgctttaccaagcaaaacgaaaaaataaaagaatatacggaaattatgacttagaggaattactacctgatattccattttctgatgtgagaagagccattatggattcgtcagaggaattaatagataattatcaggatgatgaaaccaactctatattaactttatgccgtatgattttaactatggacacgggtaaaatcataccaaaagatattgcgggaaatgcagtggctgaatcttctccattagaacatagggagagaattttgttagcagttcgtagttatcttggagagaatattgaatggactaatgaaaatgtaaatttaactataaactatttaaataacagattaaaaaaattataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143053_sequence/1
type: Sequence
persistent identity: BBa K143053 sequence
display id: BBa_K143053_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgttactagagaaaggtggtgaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143065_sequence/1
type: Sequence
persistent identity: BBa K143065 sequence
display id: BBa_K143065_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaggaggatatatttgaatacatacgaacaaattaataaagtgaaaaaaatacttcggaaacatttaaaaaataaccttattggtacttacatgtttggatcaggagttgagagtggactaaaaccaaatagtgatcttgactttttagtcgtcgtatctgaaccattgacagatcaaagtaaagaaatacttatacaaaaaattagacctatttcaaaaaaaataggagataaaagcaacttacgatatattgaattaacaattattattcagcaagaaatggtaccgtggaatcatcctcccaaacaagaatttatttatggagaatggttacaagagctttatgaacaaggatacattcctcagaaggaattaaattcagatttaaccataatgctttaccaagcaaaacgaaaaaataaaagaatatacggaaattatgacttagaggaattactacctgatattccattttctgatgtgagaagagccattatggattcgtcagaggaattaatagataattatcaggatgatgaaaccaactctatattaactttatgccgtatgattttaactatggacacgggtaaaatcataccaaaagatattgcgggaaatgcagtggctgaatcttctccattagaacatagggagagaattttgttagcagttcgtagttatcttggagagaatattgaatggactaatgaaaatgtaaatttaactataaactatttaaataacagattaaaaaaattataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143012_sequence/1
type: Sequence
persistent identity: BBa K143012 sequence
display id: BBa_K143012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143031_sequence/1
type: Sequence
persistent identity: BBa K143031 sequence
display id: BBa_K143031_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaggaggatatatttgaatacatacgaacaaattaataaagtgaaaaaaatacttcggaaacatttaaaaaataaccttattggtacttacatgtttggatcaggagttgagagtggactaaaaccaaatagtgatcttgactttttagtcgtcgtatctgaaccattgacagatcaaagtaaagaaatacttatacaaaaaattagacctatttcaaaaaaaataggagataaaagcaacttacgatatattgaattaacaattattattcagcaagaaatggtaccgtggaatcatcctcccaaacaagaatttatttatggagaatggttacaagagctttatgaacaaggatacattcctcagaaggaattaaattcagatttaaccataatgctttaccaagcaaaacgaaaaaataaaagaatatacggaaattatgacttagaggaattactacctgatattccattttctgatgtgagaagagccattatggattcgtcagaggaattaatagataattatcaggatgatgaaaccaactctatattaactttatgccgtatgattttaactatggacacgggtaaaatcataccaaaagatattgcgggaaatgcagtggctgaatcttctccattagaacatagggagagaattttgttagcagttcgtagttatcttggagagaatattgaatggactaatgaaaatgtaaatttaactataaactatttaaataacagattaaaaaaattataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143021_sequence/1
type: Sequence
persistent identity: BBa K143021 sequence
display id: BBa_K143021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaggtggtgaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z