BBa_K316022
type: ComponentDefinition
persistent identity: BBa K316022
display id: BBa_K316022
version: 1
was generated by: iGEM to SBOL conversion
description: B.subtilis transformation vector, targets amyE locus
created: 2010-10-22T11:00:00Z
modified: 2015-05-08T01:11:56Z
mutable provenance: Existing biobricks, <bbpart>BBa_K143070</bbpart>, <bbpart>BBa_K316002</bbpart>, <bbpart>BBa_K316014</bbpart> <bbpart>BBa_K143002</bbpart>
owned by: james
owned by: myers
mutable description: This vector has been designed using the amyE 5' and 3' integration sequences for integration into B.subtilis genome '''AmyE locus''' This vector has been designed using the amyE 5' <bbpart>BBa_K143008</bbpart> and 3 <bbpart>BBa_K143009</bbpart>' integration sequences for integration into ''B. subtilis'' genome. Insertion into the amyE locus provides a selection marker as the bacterium will no longer be able to breakdown starch. An iodine assay can be used to confirm integration. This phenotype makes the transformed bacterium considerably less likely to survive in natural environments. '''Chloramphenicol Resistance''' This vector also contains a positive selection marker, flanked by two dif sites. Chloramphenicol acetyltransferase <bbpart>BBa_J31005</bbpart> provides resistance to chloramphenicol antibiotic. Dif <bbpart>BBa_K316002</bbpart> sites allow excision of the antibiotic marker after integration, thus allowing the same marker to be used again or as a precaution against horizontal gene transfer. '''Blunt end cloning site''' PmeI restriction site <bbpart>BBa_K316013</bbpart> is designed as a cloning site. Due to the 8bp recognition sequence it is a rare site that can be used to cut the vector only once. Please see ???Part Design??? section for design considerations and parts used.
discontinued: false
dominant: false
experience: experience/Works
group u list: _440_
m user id: 0
owner id: 7480
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Standard biobrick assembly [http://partsregistry.org/Assembly:Standard_assembly]
star: false
creator: IC 2010 Team
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K143001
component: BBa_K316014
component: BBa_K143064
component: BBa_K143052
component: BBa_K316002
component: BBa_K143002
sequence annotation: BBa_K143001
sequence annotation: BBa_K316002
sequence annotation: BBa_K143052
sequence annotation: BBa_K143064
sequence annotation: BBa_K316014
sequence annotation: BBa_K143002
sequence: https://synbiohub.org/public/igem/BBa_K316022_sequence/1

BBa_K143001
type: Component
persistent identity: component2098372
display id: component2098372
version: 1
top level: BBa_K316022
definition: amyE 5 IS
access: public

BBa_K316014
type: Component
persistent identity: component2098393
display id: component2098393
version: 1
top level: BBa_K316022
definition: BBa_K316014
access: public

BBa_K143064
type: Component
persistent identity: component2098388
display id: component2098388
version: 1
top level: BBa_K316022
definition: CmR-T
access: public

BBa_K143052
type: Component
persistent identity: component2098379
display id: component2098379
version: 1
top level: BBa_K316022
definition: PVeg-gsiB
access: public

BBa_K316002
type: Component
persistent identity: component2098373
display id: component2098373
version: 1
top level: BBa_K316022
definition: Bs dif
access: public

BBa_K143002
type: Component
persistent identity: component2098395
display id: component2098395
version: 1
top level: BBa_K316022
definition: amyE 3 IS
access: public

BBa_K143001
type: SequenceAnnotation
persistent identity: annotation2098372
display id: annotation2098372
version: 1
top level: BBa_K316022
location: https://synbiohub.org/public/igem/BBa_K316022/annotation2098372/range2098372/1
component: BBa_K143001

https://synbiohub.org/public/igem/BBa_K316022/annotation2098372/range2098372/1
type: Range
persistent identity: range2098372
display id: range2098372
version: 1
top level: BBa_K316022
direction: direction/reverse
start: 1
end: 522
orientation: inline

BBa_K316002
type: SequenceAnnotation
persistent identity: annotation2098373
display id: annotation2098373
version: 1
top level: BBa_K316022
location: https://synbiohub.org/public/igem/BBa_K316022/annotation2098373/range2098373/1
component: BBa_K316002

https://synbiohub.org/public/igem/BBa_K316022/annotation2098373/range2098373/1
type: Range
persistent identity: range2098373
display id: range2098373
version: 1
top level: BBa_K316022
direction: direction/reverse
start: 531
end: 558
orientation: inline

BBa_K143052
type: SequenceAnnotation
persistent identity: annotation2098379
display id: annotation2098379
version: 1
top level: BBa_K316022
location: https://synbiohub.org/public/igem/BBa_K316022/annotation2098379/range2098379/1
component: BBa_K143052

https://synbiohub.org/public/igem/BBa_K316022/annotation2098379/range2098379/1
type: Range
persistent identity: range2098379
display id: range2098379
version: 1
top level: BBa_K316022
direction: direction/reverse
start: 567
end: 682
orientation: inline

BBa_K143064
type: SequenceAnnotation
persistent identity: annotation2098388
display id: annotation2098388
version: 1
top level: BBa_K316022
location: https://synbiohub.org/public/igem/BBa_K316022/annotation2098388/range2098388/1
component: BBa_K143064

https://synbiohub.org/public/igem/BBa_K316022/annotation2098388/range2098388/1
type: Range
persistent identity: range2098388
display id: range2098388
version: 1
top level: BBa_K316022
direction: direction/reverse
start: 689
end: 1485
orientation: inline

BBa_K316014
type: SequenceAnnotation
persistent identity: annotation2098393
display id: annotation2098393
version: 1
top level: BBa_K316022
location: https://synbiohub.org/public/igem/BBa_K316022/annotation2098393/range2098393/1
component: BBa_K316014

https://synbiohub.org/public/igem/BBa_K316022/annotation2098393/range2098393/1
type: Range
persistent identity: range2098393
display id: range2098393
version: 1
top level: BBa_K316022
direction: direction/reverse
start: 1494
end: 1537
orientation: inline

BBa_K143002
type: SequenceAnnotation
persistent identity: annotation2098395
display id: annotation2098395
version: 1
top level: BBa_K316022
location: https://synbiohub.org/public/igem/BBa_K316022/annotation2098395/range2098395/1
component: BBa_K143002

https://synbiohub.org/public/igem/BBa_K316022/annotation2098395/range2098395/1
type: Range
persistent identity: range2098395
display id: range2098395
version: 1
top level: BBa_K316022
direction: direction/reverse
start: 1546
end: 2547
orientation: inline

amyE 5 IS
type: ComponentDefinition
persistent identity: BBa K143001
display id: BBa_K143001
version: 1
was generated by: iGEM to SBOL conversion
description: 5??? Integration Sequence for the amyE locus of B. subtilis
created: 2008-08-26T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The 5??? integration sequence was taken from the shuttle vector pDR111 which has been used in many studies on ''B.subtilis'', in particular in the studies of transcriptional control#1 #2 #3 <biblio> #1 pmid=14597697 #2 pmid=15937167 #3 pmid=12169614 </biblio>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The 5' integration sequence can be added to the front of a Biobrick construct and the 3' integration sequence specific for this locus (Part BBa_K143002) to the rear of the Biobrick construct to allow integration of the Biobrick construct into the chromosome of the gram positive bacterium B.subtilis. The AmyE locus was the first locus used for integration into ''B.subtilis'' by Shimotsu and Henner#1 and is still commonly used in vectors such as pDR111#2, pDL#3 and their derivatives. Integration at the AmyE locus removes the ability of ''B.subtilis'' to break down starch, which can be assayed with iodine as described by Cutting and Vander-horn#4. The 5' and 3' integration sequences for the AmyE locus were used to integrate the Imperial 2008 iGEM project primary construct into the ''B.sutbilis'' chromosome. <biblio> #1 pmid=3019840 #2 pmid=14597697 #3 ''Bacillus'' Genetic Stock Center [www.bgsc.org] #4 Cutting, S M.; Vander-Horn, P B. Genetic analysis. In: Harwood C R, Cutting S M. , editors. Molecular biological methods for Bacillus. Chichester, England: John Wiley & Sons, Ltd.; 1990. pp. 27???74. </biblio>
discontinued: false
dominant: false
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: The AmyE integration sequence was taken from the vector after comparison by BLAST to the ''B.subtilis'' chromosome to identify the homologous sequences. The sequence present in both the host chromosome and the plasmid at the 5' end of the gene is the 5' sequence required for integration.
star: true
creator: Chris Hirst
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: 5' AmyE homologous sequence
sequence: https://synbiohub.org/public/igem/BBa_K143001_sequence/1

5' AmyE homologous sequence
type: SequenceAnnotation
persistent identity: annotation1974145
display id: annotation1974145
version: 1
top level: amyE 5 IS
location: https://synbiohub.org/public/igem/BBa_K143001/annotation1974145/range1974145/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K143001/annotation1974145/range1974145/1
type: Range
persistent identity: range1974145
display id: range1974145
version: 1
top level: amyE 5 IS
direction: direction/forward
start: 1
end: 522
orientation: inline

amyE 3 IS
type: ComponentDefinition
persistent identity: BBa K143002
display id: BBa_K143002
version: 1
was generated by: iGEM to SBOL conversion
description: 3??? Integration Sequence for the amyE locus of B. subtilis
created: 2008-08-27T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The 3??? integration sequence was taken from the shuttle vector pDR111 which has been used in many studies on B.subtilis, in particular in the studies of transcriptional control[1,2,3] References 1.Shimotsu H and Henner DJ. Construction of a single-copy integration vector and its use in analysis of regulation of the trp operon of Bacillus subtilis. Gene 1986; 43(1-2) 85-94. pmid:3019840. 2.Erwin KN, Nakano S, and Zuber P. Sulfate-dependent repression of genes that function in organosulfur metabolism in Bacillus subtilis requires Spx. J Bacteriol 2005 Jun; 187(12) 4042-9. doi:10.1128/JB.187.12.4042-4049.2005 pmid:15937167 3.Britton RA, Eichenberger P, Gonzalez-Pastor JE, Fawcett P, Monson R, Losick R, and Grossman AD. Genome-wide analysis of the stationary-phase sigma factor (sigma-H) regulon of Bacillus subtilis. J Bacteriol 2002 Sep; 184(17) 4881-90. pmid:12169614
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Integration sequences allow DNA to be incorporated into the chromosome of a host cell at a specific locus using leading (5') and trailing (3') DNA sequences that are the same as those at a specific locus of the chromosome. The 5' integration sequence can be added to the front of a Biobrick construct and the 3' integration sequence specific for this locus (Part BBa_K143002) to the rear of the Biobrick construct to allow integration of the Biobrick construct into the chromosome of the gram positive bacterium B.subtilis. The AmyE locus was the first locus used for integration into B.subtilis by Shimotsu and Henner[1] and is still commonly used in vectors such as pDR111[2], pDL[3] and their derivatives. Integration at the AmyE locus removes the ability of B.subtilis to break down starch, which can be assayed with iodine as described by Cutting and Vander-horn[4]. The 5' and 3' integration sequences for the AmyE locus were used to integrate the Imperial 2008 iGEM project primary construct into the B.sutbilis chromosome. References 1. Shimotsu H and Henner DJ. Construction of a single-copy integration vector and its use in analysis of regulation of the trp operon of Bacillus subtilis. Gene 1986; 43(1-2) 85-94. pmid:3019840 2.Nakano S, Kster-Schck E, Grossman AD, and Zuber P. Spx-dependent global transcriptional control is induced by thiol-specific oxidative stress in Bacillus subtilis. Proc Natl Acad Sci U S A 2003 Nov 11; 100(23) 13603-8. doi:10.1073/pnas.2235180100 pmid:14597697 3.Bacillus Genetic Stock Center; www.bgsc.org 4.Cutting, S M.; Vander-Horn, P B. Genetic analysis. In: Harwood C R, Cutting S M. , editors. Molecular biological methods for Bacillus. Chichester, England: John Wiley & Sons, Ltd.; 1990. pp. 27???74.
discontinued: false
dominant: false
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: The AmyE integration sequence was taken from the vector after comparison by BLAST to the B.subtilis chromosome to identify the homologous sequences. The sequence present in both the host chromosome and the plasmid at the 3' end of the gene is the 3' sequence required for integration
star: true
creator: Chris Hirst
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: 3' AmyE homologous sequence
sequence: https://synbiohub.org/public/igem/BBa_K143002_sequence/1

3' AmyE homologous sequence
type: SequenceAnnotation
persistent identity: annotation1974146
display id: annotation1974146
version: 1
top level: amyE 3 IS
location: https://synbiohub.org/public/igem/BBa_K143002/annotation1974146/range1974146/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K143002/annotation1974146/range1974146/1
type: Range
persistent identity: range1974146
display id: range1974146
version: 1
top level: amyE 3 IS
direction: direction/forward
start: 1
end: 1005
orientation: inline

PVeg-gsiB
type: ComponentDefinition
persistent identity: BBa K143052
display id: BBa_K143052
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter Pveg and RBS gsiB for B. subtilis
created: 2008-10-05T11:00:00Z
modified: 2015-05-08T01:10:24Z
mutable provenance: Pveg-gsiB was synthesised by GeneArt
owned by: james
owned by: myers
mutable description: Constitutive promoter veg(<bbpart>BBa_K143012</bbpart>) coupled to the strong Ribosome Binding Site gsiB(<bbpart>BBa_K143020</bbpart>) from ''B. subtilis''. Pveg-gsiB can be used in the context of a Polymerases per second (PoPS) output generator '''To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix1. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.'''
discontinued: false
dominant: false
experience: experience/None
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The sequence of Pveg was obtained from the DBTBS1 and RBS-gsiB were obtained from papers2 and the sequence synthesised by GeneArt
star: false
creator: Chris Hirst
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_K143012
component: BBa_K143020
sequence annotation: BBa_K143012
sequence annotation: BBa_K143020
sequence: https://synbiohub.org/public/igem/BBa_K143052_sequence/1

BBa_K143012
type: Component
persistent identity: component1981492
display id: component1981492
version: 1
top level: PVeg-gsiB
definition: Pveg
access: public

BBa_K143020
type: Component
persistent identity: component1981494
display id: component1981494
version: 1
top level: PVeg-gsiB
definition: RBS-GsiB
access: public

BBa_K143012
type: SequenceAnnotation
persistent identity: annotation1981492
display id: annotation1981492
version: 1
top level: PVeg-gsiB
location: https://synbiohub.org/public/igem/BBa_K143052/annotation1981492/range1981492/1
component: BBa_K143012

https://synbiohub.org/public/igem/BBa_K143052/annotation1981492/range1981492/1
type: Range
persistent identity: range1981492
display id: range1981492
version: 1
top level: PVeg-gsiB
direction: direction/reverse
start: 1
end: 97
orientation: inline

BBa_K143020
type: SequenceAnnotation
persistent identity: annotation1981494
display id: annotation1981494
version: 1
top level: PVeg-gsiB
location: https://synbiohub.org/public/igem/BBa_K143052/annotation1981494/range1981494/1
component: BBa_K143020

https://synbiohub.org/public/igem/BBa_K143052/annotation1981494/range1981494/1
type: Range
persistent identity: range1981494
display id: range1981494
version: 1
top level: PVeg-gsiB
direction: direction/reverse
start: 106
end: 116
orientation: inline

CmR-T
type: ComponentDefinition
persistent identity: BBa K143064
display id: BBa_K143064
version: 1
was generated by: iGEM to SBOL conversion
description: Chloramphenicol resistance protein - Terminator
created: 2008-10-08T11:00:00Z
modified: 2015-05-08T01:10:24Z
mutable provenance: The Chloraphemicol acetyltransferase and double terminator were taken both taken from the registry.
owned by: james
owned by: myers
mutable description: Chloraphemicol acetyltransferase protein(<bbpart>BBa_J31005</bbpart>) coupled to the double terminator (<bbpart>BBa_B0015</bbpart>). Chloraphemicol acetyltransferase confers resistance to Chloraphemicol. The double terminator is the most commonly used terminator and is a combination of parts <bbpart>BBa_B0010</bbpart> and <bbpart>BBa_B0012</bbpart>. The double terminator allows the CAT to be incorporated into a closed transcriptional unit.
discontinued: false
dominant: true
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Chloraphemicol acetyltransferase is an exisiting registry protein. The double terminator is the most commonly used registry termiantor.
star: true
creator: Chris Hirst
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J31005
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_J31005
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K143064_sequence/1

BBa_J31005
type: Component
persistent identity: component1980003
display id: component1980003
version: 1
top level: CmR-T
definition: CmR
access: public

BBa_B0012
type: Component
persistent identity: component1980006
display id: component1980006
version: 1
top level: CmR-T
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1980004
display id: component1980004
version: 1
top level: CmR-T
definition: BBa_B0010
access: public

BBa_J31005
type: SequenceAnnotation
persistent identity: annotation1980003
display id: annotation1980003
version: 1
top level: CmR-T
location: https://synbiohub.org/public/igem/BBa_K143064/annotation1980003/range1980003/1
component: BBa_J31005

https://synbiohub.org/public/igem/BBa_K143064/annotation1980003/range1980003/1
type: Range
persistent identity: range1980003
display id: range1980003
version: 1
top level: CmR-T
direction: direction/reverse
start: 1
end: 660
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1980004
display id: annotation1980004
version: 1
top level: CmR-T
location: https://synbiohub.org/public/igem/BBa_K143064/annotation1980004/range1980004/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K143064/annotation1980004/range1980004/1
type: Range
persistent identity: range1980004
display id: range1980004
version: 1
top level: CmR-T
direction: direction/forward
start: 669
end: 748
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1980006
display id: annotation1980006
version: 1
top level: CmR-T
location: https://synbiohub.org/public/igem/BBa_K143064/annotation1980006/range1980006/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K143064/annotation1980006/range1980006/1
type: Range
persistent identity: range1980006
display id: range1980006
version: 1
top level: CmR-T
direction: direction/forward
start: 757
end: 797
orientation: inline

Bs dif
type: ComponentDefinition
persistent identity: BBa K316002
display id: BBa_K316002
version: 1
was generated by: iGEM to SBOL conversion
description: dif excision site from B. subtilis
created: 2010-10-19T11:00:00Z
modified: 2015-05-08T01:11:56Z
mutable provenance: The dif sites were made by annealing synthestised oligoes.
owned by: james
owned by: myers
mutable description: Dif sites are naturally found in B.subtilis and are used by this organism during genome replication.
discontinued: false
dominant: false
experience: experience/None
group u list: _440_
m user id: 0
owner id: 7480
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The dif site was made by oligos designed to make overhangs for EcoRI and SpeI ( and ) or XbaI and PstI ( and ) to be used in standard Biobrick or 3A cloning.
star: false
creator: IC 2010 Team
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K316002_sequence/1

BBa_K316014
type: ComponentDefinition
persistent identity: BBa K316014
display id: BBa_K316014
version: 1
was generated by: iGEM to SBOL conversion
description: Dif sequence followed by PmeI recognition site
created: 2010-10-22T11:00:00Z
modified: 2015-05-08T01:11:56Z
mutable provenance: Oligonucleotide synthesis of single stranded primers.
owned by: james
owned by: myers
mutable description: This composite part of <bbpart>BBa_K143000</bbpart> and <bbpart>BBa_K143013</bbpart>. The dif site can be used in conjunction with another dif site in another part of the vector to remove a sequence between the two dif sites. PmeI site can be used for blunt end cloning of a DNA sequence behind the dif site. Please see ???Part Design??? section for design considerations and parts used.
discontinued: false
dominant: false
experience: experience/None
group u list: _440_
m user id: 0
owner id: 7480
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: This part was designed to be cloned using standard biobrick methods. Two single stranded, synthetic oligos were annealed to produce a double stranded DNA sequence with single stranded overhangs identical to the product of digestion by EcoRI and SpeI. Thus compatible with biobrick cloning methods.
star: false
creator: IC 2010 Team
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_K316013
component: BBa_K316002
sequence annotation: BBa_K316002
sequence annotation: BBa_K316013
sequence: https://synbiohub.org/public/igem/BBa_K316014_sequence/1

BBa_K316013
type: Component
persistent identity: component2098249
display id: component2098249
version: 1
top level: BBa_K316014
definition: PmeI site
access: public

BBa_K316002
type: Component
persistent identity: component2098246
display id: component2098246
version: 1
top level: BBa_K316014
definition: Bs dif
access: public

BBa_K316002
type: SequenceAnnotation
persistent identity: annotation2098246
display id: annotation2098246
version: 1
top level: BBa_K316014
location: https://synbiohub.org/public/igem/BBa_K316014/annotation2098246/range2098246/1
component: BBa_K316002

https://synbiohub.org/public/igem/BBa_K316014/annotation2098246/range2098246/1
type: Range
persistent identity: range2098246
display id: range2098246
version: 1
top level: BBa_K316014
direction: direction/reverse
start: 1
end: 28
orientation: inline

BBa_K316013
type: SequenceAnnotation
persistent identity: annotation2098249
display id: annotation2098249
version: 1
top level: BBa_K316014
location: https://synbiohub.org/public/igem/BBa_K316014/annotation2098249/range2098249/1
component: BBa_K316013

https://synbiohub.org/public/igem/BBa_K316014/annotation2098249/range2098249/1
type: Range
persistent identity: range2098249
display id: range2098249
version: 1
top level: BBa_K316014
direction: direction/reverse
start: 37
end: 44
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

Pveg
type: ComponentDefinition
persistent identity: BBa K143012
display id: BBa_K143012
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter veg a constitutive promoter for B. subtilis
created: 2008-09-10T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The Pveg promoter was suggested to us by Dr. Jan-Willem Veening of Newcastle University. This sequence supplied was compared to that of the DBTBS database#3 then a section containing the binding site synthesised by Geneart.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Pveg is a constitutive promoter that constitutively expresses the P43 protein in ''B.subtilis''. Pveg contains binding sites for the ''B.sutbilis'' major sigma factor#1. Pveg in ''B.subtilis'' utilises two binding sites to cause high expression of genes#2, however our Pveg is lacking the upstream site to give a medium level of gene expression. It has been noted that the sporulation master regulatoion factor spoOA interacts with Pveg though it is not known how#3. The context with which we used the promoter Pveg is as a '''Polymerase Per Second''' (PoPS) generator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The biobrick part was designed to include a single binding site for the ''B.subtilis major sigma factor. In addition the biobrick standard was applied to the promoter Pveg sequence.
star: false
creator: James Chappell
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: Sigma A-35
sequence annotation: Sigma A -10
sequence: https://synbiohub.org/public/igem/BBa_K143012_sequence/1

Sigma A-35
type: SequenceAnnotation
persistent identity: annotation1975704
display id: annotation1975704
version: 1
top level: Pveg
location: https://synbiohub.org/public/igem/BBa_K143012/annotation1975704/range1975704/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K143012/annotation1975704/range1975704/1
type: Range
persistent identity: range1975704
display id: range1975704
version: 1
top level: Pveg
direction: direction/forward
start: 63
end: 68
orientation: inline

Sigma A -10
type: SequenceAnnotation
persistent identity: annotation1975705
display id: annotation1975705
version: 1
top level: Pveg
location: https://synbiohub.org/public/igem/BBa_K143012/annotation1975705/range1975705/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K143012/annotation1975705/range1975705/1
type: Range
persistent identity: range1975705
display id: range1975705
version: 1
top level: Pveg
direction: direction/forward
start: 86
end: 91
orientation: inline

RBS-GsiB
type: ComponentDefinition
persistent identity: BBa K143020
display id: BBa_K143020
version: 1
was generated by: iGEM to SBOL conversion
description: GsiB ribosome binding site (RBS) for B. subtilis
created: 2008-09-14T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The sequence was taken from a previous research paper [1] and was constructed by Geneart
owned by: james
owned by: myers
mutable description: GsiB is an endogenous ribosome binding site from ''B.subtilis''. The sequence of the gsiB ribosome binding site is '''AAAGGAGG''' which is complementary to the sequence '''UUUCCUCC''' from the 3' region of the 16s rRNA from ''B.subtilis''. GsiB is an endogenous ribosome binding site (RBS) from ''B.subtilis''. The sequence of the gsiB ribosome binding site is '''AAAGGAGG''' which is complementary to the sequence '''UUUCCUCC''' from the 3' region of the 16s rRNA from ''B.subtilis''. Previous research showed that the predicted binding energy of the 16s rRNA to the RBS is -9.3kcal.
discontinued: false
dominant: false
experience: experience/None
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: In order to ensure that the RBS is functional the actual ribosome binding site was maintained and the distance between the RBS and the start codon maintained. In order to conform to the biobrick standard the sequence flanking the RBS had to be changed but the distance between the promoter and RBS, and start codon and RBS was maintained.
star: false
creator: James Chappell
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K143020_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1975872
display id: annotation1975872
version: 1
top level: RBS-GsiB
location: https://synbiohub.org/public/igem/BBa_K143020/annotation1975872/range1975872/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K143020/annotation1975872/range1975872/1
type: Range
persistent identity: range1975872
display id: range1975872
version: 1
top level: RBS-GsiB
direction: direction/forward
start: 2
end: 8
orientation: inline

CmR
type: ComponentDefinition
persistent identity: BBa J31005
display id: BBa_J31005
version: 1
was generated by: iGEM to SBOL conversion
description: chloramphenicol acetyltransferase (forwards, CmF) [cf. BBa_J31004]
created: 2006-07-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
mutable provenance: pSB1AC3
owned by: james
owned by: myers
mutable description: When a promoter and an RBS are in front of the gene, the cell will express Chloramphenicol resistance. Because it contains full biobrick ends, this part can be used to easily add chloramphenicol resistance to any part without changing plasmid vectors.
discontinued: false
dominant: true
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 918
owning group id: 61
sample status: In stock
status: status/Available
bookmark: true
mutable notes: This part is cloned into pSB1A2.
star: true
creator: Erin Zwack, Sabriya Rosemond
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CmR gene
sequence: https://synbiohub.org/public/igem/BBa_J31005_sequence/1

CmR gene
type: SequenceAnnotation
persistent identity: annotation1884999
display id: annotation1884999
version: 1
top level: CmR
location: https://synbiohub.org/public/igem/BBa_J31005/annotation1884999/range1884999/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J31005/annotation1884999/range1884999/1
type: Range
persistent identity: range1884999
display id: range1884999
version: 1
top level: CmR
direction: direction/forward
start: 1
end: 660
orientation: inline

PmeI site
type: ComponentDefinition
persistent identity: BBa K316013
display id: BBa_K316013
version: 1
was generated by: iGEM to SBOL conversion
description: 8bp recognition sequence for PmeI restriction endonuclease
created: 2010-10-22T11:00:00Z
modified: 2015-05-08T01:11:56Z
mutable provenance: This is a planning part
owned by: james
owned by: myers
mutable description: Information about PmeI restriction endonuclease is available at [http://www.neb.com/nebecomm/products/productR0560.asp]. The recognition site is a 8bp sequence GTTTAAAC. Pme produces a blunt cut after GTTT.
discontinued: false
dominant: false
experience: experience/None
group u list: _440_
m user id: 0
owner id: 7480
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This is a planning part
star: false
creator: IC 2010 Team
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: 5' product
sequence annotation: 3' product
sequence: https://synbiohub.org/public/igem/BBa_K316013_sequence/1

5' product
type: SequenceAnnotation
persistent identity: annotation2098160
display id: annotation2098160
version: 1
top level: PmeI site
location: https://synbiohub.org/public/igem/BBa_K316013/annotation2098160/range2098160/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K316013/annotation2098160/range2098160/1
type: Range
persistent identity: range2098160
display id: range2098160
version: 1
top level: PmeI site
direction: direction/forward
start: 1
end: 4
orientation: inline

3' product
type: SequenceAnnotation
persistent identity: annotation2098166
display id: annotation2098166
version: 1
top level: PmeI site
location: https://synbiohub.org/public/igem/BBa_K316013/annotation2098166/range2098166/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K316013/annotation2098166/range2098166/1
type: Range
persistent identity: range2098166
display id: range2098166
version: 1
top level: PmeI site
direction: direction/forward
start: 5
end: 8
orientation: inline

https://synbiohub.org/public/igem/BBa_K316022_sequence/1
type: Sequence
persistent identity: BBa K316022 sequence
display id: BBa_K316022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtttgcaaaacgattcaaaacctctttactgccgttattcgctggatttttattgctgtttcatttggttctggcaggaccggcggctgcgagtgctgaaacggcgaacaaatcgaatgagcttacagcaccgtcgatcaaaagcggaaccattcttcatgcatggaattggtcgttcaatacgttaaaacacaatatgaaggatattcatgatgcaggatatacagccattcagacatctccgattaaccaagtaaaggaagggaatcaaggagataaaagcatgtcgaactggtactggctgtatcagccgacatcgtatcaaattggcaaccgttacttaggtactgaacaagaatttaaagaaatgtgtgcagccgctgaagaatatggcataaaggtcattgttgacgcggtcatcaatcataccaccagtgattatgccgcgatttccaatgaggttaagagtattccaaactggacacatggaaacacacaaattaaaaactggtctgatcgatactagagatctcctagaatatatattatgtaaacttactagagaattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgttactagagtaaaggaggaatactagatggagaaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatccggaatttcgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcaccatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgattcaggttcatcatgccgtttgtgatggcttccatgtcggcagaatgcttaatgaattacaacagtactgcgatgagtggcagggcggggcgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagatctcctagaatatatattatgtaaacttactagaggaaatttctactagagatccgtttaggctgggcggtgatagcttctcgttcaggcagtacgcctcttttcttttccagacctgagggaggcggaaatggtgtgaggttcccggggaaaagccaaataggcgatcgcgggagtgctttatttgaagatcaggctatcactgcggtcaatagatttcacaatgtgatggctggacagcctgaggaactctcgaacccgaatggaaacaaccagatatttatgaatcagcgcggctcacatggcgttgtgctggcaaatgcaggttcatcctctgtctctatcaatacggcaacaaaattgcctgatggcaggtatgacaataaagctggagcgggttcatttcaagtgaacgatggtaaactgacaggcacgatcaatgccaggtctgtagctgtgctttatcctgatgatattgcaaaagcgcctcatgttttccttgagaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143001_sequence/1
type: Sequence
persistent identity: BBa K143001 sequence
display id: BBa_K143001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtttgcaaaacgattcaaaacctctttactgccgttattcgctggatttttattgctgtttcatttggttctggcaggaccggcggctgcgagtgctgaaacggcgaacaaatcgaatgagcttacagcaccgtcgatcaaaagcggaaccattcttcatgcatggaattggtcgttcaatacgttaaaacacaatatgaaggatattcatgatgcaggatatacagccattcagacatctccgattaaccaagtaaaggaagggaatcaaggagataaaagcatgtcgaactggtactggctgtatcagccgacatcgtatcaaattggcaaccgttacttaggtactgaacaagaatttaaagaaatgtgtgcagccgctgaagaatatggcataaaggtcattgttgacgcggtcatcaatcataccaccagtgattatgccgcgatttccaatgaggttaagagtattccaaactggacacatggaaacacacaaattaaaaactggtctgatcga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143002_sequence/1
type: Sequence
persistent identity: BBa K143002 sequence
display id: BBa_K143002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atccgtttaggctgggcggtgatagcttctcgttcaggcagtacgcctcttttcttttccagacctgagggaggcggaaatggtgtgaggttcccggggaaaagccaaataggcgatcgcgggagtgctttatttgaagatcaggctatcactgcggtcaatagatttcacaatgtgatggctggacagcctgaggaactctcgaacccgaatggaaacaaccagatatttatgaatcagcgcggctcacatggcgttgtgctggcaaatgcaggttcatcctctgtctctatcaatacggcaacaaaattgcctgatggcaggtatgacaataaagctggagcgggttcatttcaagtgaacgatggtaaactgacaggcacgatcaatgccaggtctgtagctgtgctttatcctgatgatattgcaaaagcgcctcatgttttccttgagaattacaaaacaggtgtaacacattctttcaatgatcaactgacgattaccttgcgtgcagatgcgaatacaacaaaagccgtttatcaaatcaataatggaccagagacggcgtttaaggatggagatcaattcacaatcggaaaaggagatccatttggcaaaacatacaccatcatgttaaaaggaacgaacagtgatggtgtaacgaggaccgagaaatacagttttgttaaaagagatccagcgtcggccaaaaccatcggctatcaaaatccgaatcattggagccaggtaaatgcttatatctataaacatgatgggagccgagtaattgaattgaccggatcttggcctggaaaaccaatgactaaaaatgcagacggaatttacacgctgacgctgcctgcggacacggatacaaccaacgcaaaagtgatttttaataatggcagcgcccaagtgcccggtcagaatcagcctggctttgattacgtgctaaatggtttatataatgactcgggcttaagcggttctcttccccattga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143052_sequence/1
type: Sequence
persistent identity: BBa K143052 sequence
display id: BBa_K143052_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgttactagagtaaaggaggaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143064_sequence/1
type: Sequence
persistent identity: BBa K143064 sequence
display id: BBa_K143064_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggagaaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatccggaatttcgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcaccatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgattcaggttcatcatgccgtttgtgatggcttccatgtcggcagaatgcttaatgaattacaacagtactgcgatgagtggcagggcggggcgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K316002_sequence/1
type: Sequence
persistent identity: BBa K316002 sequence
display id: BBa_K316002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atctcctagaatatatattatgtaaact
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K316014_sequence/1
type: Sequence
persistent identity: BBa K316014 sequence
display id: BBa_K316014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atctcctagaatatatattatgtaaacttactagaggaaatttc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143012_sequence/1
type: Sequence
persistent identity: BBa K143012 sequence
display id: BBa_K143012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143020_sequence/1
type: Sequence
persistent identity: BBa K143020 sequence
display id: BBa_K143020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taaaggaggaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J31005_sequence/1
type: Sequence
persistent identity: BBa J31005 sequence
display id: BBa_J31005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggagaaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatccggaatttcgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcaccatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgattcaggttcatcatgccgtttgtgatggcttccatgtcggcagaatgcttaatgaattacaacagtactgcgatgagtggcagggcggggcgtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K316013_sequence/1
type: Sequence
persistent identity: BBa K316013 sequence
display id: BBa_K316013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaaatttc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z