BBa_K316023
type: ComponentDefinition
persistent identity: BBa K316023
display id: BBa_K316023
version: 1
was generated by: iGEM to SBOL conversion
description: Chloraphenicol resistance gene with 5amyE
created: 2010-10-22T11:00:00Z
modified: 2015-05-08T01:11:56Z
mutable provenance: iobrick parts <bbpart>BBa_K143070</bbpart> (which contains <bbpart>BBa_K143001</bbpart>, <bbpart>BBa_K316012</bbpart>, <bbpart>BBa_K143021</bbpart>) and <bbpart>BBa_K143064</bbpart> (which contains <bbpart>BBa_K143031</bbpart>, <bbpart>BBa_B0015</bbpart>)
owned by: james
owned by: myers
mutable description: <bbpart>BBa_K143001</bbpart> integration sequence allows integration into B. subtilis genome, which disrupts ability to breakdown starch. In order for integration to occur, 3' amyE integration sequence <bbpart>BBa_K143002</bbpart> is also required. This part can be used as the 5' start of an amyE integration vector, the genes of interest can then be attached to the 3' end, followed by the 3' amye integratio sequwnce <bbpart>BBa_K143002</bbpart>.
discontinued: false
dominant: false
experience: experience/None
group u list: _440_
m user id: 0
owner id: 7480
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Parts combined by standard biobrick assembly [http://partsregistry.org/Assembly:Standard_assembly]
star: false
creator: IC 2010 Team
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K143064
component: BBa_K143070
sequence annotation: BBa_K143070
sequence annotation: BBa_K143064
sequence: https://synbiohub.org/public/igem/BBa_K316023_sequence/1

BBa_K143064
type: Component
persistent identity: component2097185
display id: component2097185
version: 1
top level: BBa_K316023
definition: CmR-T
access: public

BBa_K143070
type: Component
persistent identity: component2097176
display id: component2097176
version: 1
top level: BBa_K316023
definition: BBa_K143070
access: public

BBa_K143070
type: SequenceAnnotation
persistent identity: annotation2097176
display id: annotation2097176
version: 1
top level: BBa_K316023
location: https://synbiohub.org/public/igem/BBa_K316023/annotation2097176/range2097176/1
component: BBa_K143070

https://synbiohub.org/public/igem/BBa_K316023/annotation2097176/range2097176/1
type: Range
persistent identity: range2097176
display id: range2097176
version: 1
top level: BBa_K316023
direction: direction/reverse
start: 1
end: 647
orientation: inline

BBa_K143064
type: SequenceAnnotation
persistent identity: annotation2097185
display id: annotation2097185
version: 1
top level: BBa_K316023
location: https://synbiohub.org/public/igem/BBa_K316023/annotation2097185/range2097185/1
component: BBa_K143064

https://synbiohub.org/public/igem/BBa_K316023/annotation2097185/range2097185/1
type: Range
persistent identity: range2097185
display id: range2097185
version: 1
top level: BBa_K316023
direction: direction/reverse
start: 654
end: 1450
orientation: inline

CmR-T
type: ComponentDefinition
persistent identity: BBa K143064
display id: BBa_K143064
version: 1
was generated by: iGEM to SBOL conversion
description: Chloramphenicol resistance protein - Terminator
created: 2008-10-08T11:00:00Z
modified: 2015-05-08T01:10:24Z
mutable provenance: The Chloraphemicol acetyltransferase and double terminator were taken both taken from the registry.
owned by: james
owned by: myers
mutable description: Chloraphemicol acetyltransferase protein(<bbpart>BBa_J31005</bbpart>) coupled to the double terminator (<bbpart>BBa_B0015</bbpart>). Chloraphemicol acetyltransferase confers resistance to Chloraphemicol. The double terminator is the most commonly used terminator and is a combination of parts <bbpart>BBa_B0010</bbpart> and <bbpart>BBa_B0012</bbpart>. The double terminator allows the CAT to be incorporated into a closed transcriptional unit.
discontinued: false
dominant: true
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Chloraphemicol acetyltransferase is an exisiting registry protein. The double terminator is the most commonly used registry termiantor.
star: true
creator: Chris Hirst
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J31005
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_J31005
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K143064_sequence/1

BBa_J31005
type: Component
persistent identity: component1980003
display id: component1980003
version: 1
top level: CmR-T
definition: CmR
access: public

BBa_B0012
type: Component
persistent identity: component1980006
display id: component1980006
version: 1
top level: CmR-T
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1980004
display id: component1980004
version: 1
top level: CmR-T
definition: BBa_B0010
access: public

BBa_J31005
type: SequenceAnnotation
persistent identity: annotation1980003
display id: annotation1980003
version: 1
top level: CmR-T
location: https://synbiohub.org/public/igem/BBa_K143064/annotation1980003/range1980003/1
component: BBa_J31005

https://synbiohub.org/public/igem/BBa_K143064/annotation1980003/range1980003/1
type: Range
persistent identity: range1980003
display id: range1980003
version: 1
top level: CmR-T
direction: direction/reverse
start: 1
end: 660
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1980004
display id: annotation1980004
version: 1
top level: CmR-T
location: https://synbiohub.org/public/igem/BBa_K143064/annotation1980004/range1980004/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K143064/annotation1980004/range1980004/1
type: Range
persistent identity: range1980004
display id: range1980004
version: 1
top level: CmR-T
direction: direction/forward
start: 669
end: 748
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1980006
display id: annotation1980006
version: 1
top level: CmR-T
location: https://synbiohub.org/public/igem/BBa_K143064/annotation1980006/range1980006/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K143064/annotation1980006/range1980006/1
type: Range
persistent identity: range1980006
display id: range1980006
version: 1
top level: CmR-T
direction: direction/forward
start: 757
end: 797
orientation: inline

BBa_K143070
type: ComponentDefinition
persistent identity: BBa K143070
display id: BBa_K143070
version: 1
was generated by: iGEM to SBOL conversion
description: AmyE integratable PoPS generator (Pveg-spoVG)
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:10:24Z
mutable provenance: The amyE 5' integration sequence was PCR cloned from the ''B. subtilis'' integration vector utilising Pfu DNA polymerase and cloned into a BioBrick with the Pveg-spoVG that was synthesised by GeneArt.
owned by: james
owned by: myers
mutable description: AmyE 5' Integration sequence(<bbpart>BBa_K143001</bbpart>) coupled to the PoPS generator Pveg-spoVG (<bbpart>BBa_K143053</bbpart>). The amyE 5' integration sequence allows integration into the ''B. subtilis'' genome at the amyE locus if the 3' amyE integration sequence(<bbpart>BBa_K143002</bbpart>) is cloned onto the 3' end of the construct. The Pveg-spoVG promoter and RBS for ''B. subtilis'' constitutively generate a PoPS output.
discontinued: false
dominant: false
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The amyE 5' integration sequence was PCR cloned from the ''B. subtilis'' integration vector pDR111 utilising Pfu DNA polymerase. The sequence of Pveg-spoVG was obtained from papers.
star: false
creator: Chris Hirst
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_K143021
component: BBa_K143012
component: BBa_K143001
sequence annotation: BBa_K143001
sequence annotation: BBa_K143012
sequence annotation: BBa_K143021
sequence: https://synbiohub.org/public/igem/BBa_K143070_sequence/1

BBa_K143021
type: Component
persistent identity: component1990786
display id: component1990786
version: 1
top level: BBa_K143070
definition: RBS-spoVG
access: public

BBa_K143012
type: Component
persistent identity: component1990784
display id: component1990784
version: 1
top level: BBa_K143070
definition: Pveg
access: public

BBa_K143001
type: Component
persistent identity: component1990781
display id: component1990781
version: 1
top level: BBa_K143070
definition: amyE 5 IS
access: public

BBa_K143001
type: SequenceAnnotation
persistent identity: annotation1990781
display id: annotation1990781
version: 1
top level: BBa_K143070
location: https://synbiohub.org/public/igem/BBa_K143070/annotation1990781/range1990781/1
component: BBa_K143001

https://synbiohub.org/public/igem/BBa_K143070/annotation1990781/range1990781/1
type: Range
persistent identity: range1990781
display id: range1990781
version: 1
top level: BBa_K143070
direction: direction/reverse
start: 1
end: 522
orientation: inline

BBa_K143012
type: SequenceAnnotation
persistent identity: annotation1990784
display id: annotation1990784
version: 1
top level: BBa_K143070
location: https://synbiohub.org/public/igem/BBa_K143070/annotation1990784/range1990784/1
component: BBa_K143012

https://synbiohub.org/public/igem/BBa_K143070/annotation1990784/range1990784/1
type: Range
persistent identity: range1990784
display id: range1990784
version: 1
top level: BBa_K143070
direction: direction/reverse
start: 531
end: 627
orientation: inline

BBa_K143021
type: SequenceAnnotation
persistent identity: annotation1990786
display id: annotation1990786
version: 1
top level: BBa_K143070
location: https://synbiohub.org/public/igem/BBa_K143070/annotation1990786/range1990786/1
component: BBa_K143021

https://synbiohub.org/public/igem/BBa_K143070/annotation1990786/range1990786/1
type: Range
persistent identity: range1990786
display id: range1990786
version: 1
top level: BBa_K143070
direction: direction/reverse
start: 636
end: 647
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

amyE 5 IS
type: ComponentDefinition
persistent identity: BBa K143001
display id: BBa_K143001
version: 1
was generated by: iGEM to SBOL conversion
description: 5??? Integration Sequence for the amyE locus of B. subtilis
created: 2008-08-26T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The 5??? integration sequence was taken from the shuttle vector pDR111 which has been used in many studies on ''B.subtilis'', in particular in the studies of transcriptional control#1 #2 #3 <biblio> #1 pmid=14597697 #2 pmid=15937167 #3 pmid=12169614 </biblio>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The 5' integration sequence can be added to the front of a Biobrick construct and the 3' integration sequence specific for this locus (Part BBa_K143002) to the rear of the Biobrick construct to allow integration of the Biobrick construct into the chromosome of the gram positive bacterium B.subtilis. The AmyE locus was the first locus used for integration into ''B.subtilis'' by Shimotsu and Henner#1 and is still commonly used in vectors such as pDR111#2, pDL#3 and their derivatives. Integration at the AmyE locus removes the ability of ''B.subtilis'' to break down starch, which can be assayed with iodine as described by Cutting and Vander-horn#4. The 5' and 3' integration sequences for the AmyE locus were used to integrate the Imperial 2008 iGEM project primary construct into the ''B.sutbilis'' chromosome. <biblio> #1 pmid=3019840 #2 pmid=14597697 #3 ''Bacillus'' Genetic Stock Center [www.bgsc.org] #4 Cutting, S M.; Vander-Horn, P B. Genetic analysis. In: Harwood C R, Cutting S M. , editors. Molecular biological methods for Bacillus. Chichester, England: John Wiley & Sons, Ltd.; 1990. pp. 27???74. </biblio>
discontinued: false
dominant: false
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: The AmyE integration sequence was taken from the vector after comparison by BLAST to the ''B.subtilis'' chromosome to identify the homologous sequences. The sequence present in both the host chromosome and the plasmid at the 5' end of the gene is the 5' sequence required for integration.
star: true
creator: Chris Hirst
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: 5' AmyE homologous sequence
sequence: https://synbiohub.org/public/igem/BBa_K143001_sequence/1

5' AmyE homologous sequence
type: SequenceAnnotation
persistent identity: annotation1974145
display id: annotation1974145
version: 1
top level: amyE 5 IS
location: https://synbiohub.org/public/igem/BBa_K143001/annotation1974145/range1974145/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K143001/annotation1974145/range1974145/1
type: Range
persistent identity: range1974145
display id: range1974145
version: 1
top level: amyE 5 IS
direction: direction/forward
start: 1
end: 522
orientation: inline

Pveg
type: ComponentDefinition
persistent identity: BBa K143012
display id: BBa_K143012
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter veg a constitutive promoter for B. subtilis
created: 2008-09-10T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The Pveg promoter was suggested to us by Dr. Jan-Willem Veening of Newcastle University. This sequence supplied was compared to that of the DBTBS database#3 then a section containing the binding site synthesised by Geneart.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Pveg is a constitutive promoter that constitutively expresses the P43 protein in ''B.subtilis''. Pveg contains binding sites for the ''B.sutbilis'' major sigma factor#1. Pveg in ''B.subtilis'' utilises two binding sites to cause high expression of genes#2, however our Pveg is lacking the upstream site to give a medium level of gene expression. It has been noted that the sporulation master regulatoion factor spoOA interacts with Pveg though it is not known how#3. The context with which we used the promoter Pveg is as a '''Polymerase Per Second''' (PoPS) generator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The biobrick part was designed to include a single binding site for the ''B.subtilis major sigma factor. In addition the biobrick standard was applied to the promoter Pveg sequence.
star: false
creator: James Chappell
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: Sigma A-35
sequence annotation: Sigma A -10
sequence: https://synbiohub.org/public/igem/BBa_K143012_sequence/1

Sigma A-35
type: SequenceAnnotation
persistent identity: annotation1975704
display id: annotation1975704
version: 1
top level: Pveg
location: https://synbiohub.org/public/igem/BBa_K143012/annotation1975704/range1975704/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K143012/annotation1975704/range1975704/1
type: Range
persistent identity: range1975704
display id: range1975704
version: 1
top level: Pveg
direction: direction/forward
start: 63
end: 68
orientation: inline

Sigma A -10
type: SequenceAnnotation
persistent identity: annotation1975705
display id: annotation1975705
version: 1
top level: Pveg
location: https://synbiohub.org/public/igem/BBa_K143012/annotation1975705/range1975705/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K143012/annotation1975705/range1975705/1
type: Range
persistent identity: range1975705
display id: range1975705
version: 1
top level: Pveg
direction: direction/forward
start: 86
end: 91
orientation: inline

RBS-spoVG
type: ComponentDefinition
persistent identity: BBa K143021
display id: BBa_K143021
version: 1
was generated by: iGEM to SBOL conversion
description: SpoVG ribosome binding site (RBS) for B. subtilis
created: 2008-09-16T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The sequence was taken from a previous research paper [1] and was constructed by Geneart.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Description: SpoVG is an endogenous ribosome binding site from B.subtilis. The sequence of the spoVG ribosome binding site is AAAGGUGGUGA which is complementary to the sequence UUUCCUCCACU from the 3' region of the 16s rRNA from B.subtilis. Previous research showed that the predicted binding energy of the 16s rRNA to the RBS is -19kcal 1
discontinued: false
dominant: true
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In order to ensure that the RBS is functional the actual ribosome binding site was maintained and the distance between the RBS and the start codon maintained. In order to conform to the biobrick standard the sequence flanking the RBS had to be changed but the distance between the promoter and RBS, and start codon and RBS was maintained.
star: false
creator: James Chappell
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K143021_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1975997
display id: annotation1975997
version: 1
top level: RBS-spoVG
location: https://synbiohub.org/public/igem/BBa_K143021/annotation1975997/range1975997/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K143021/annotation1975997/range1975997/1
type: Range
persistent identity: range1975997
display id: range1975997
version: 1
top level: RBS-spoVG
direction: direction/forward
start: 1
end: 12
orientation: inline

CmR
type: ComponentDefinition
persistent identity: BBa J31005
display id: BBa_J31005
version: 1
was generated by: iGEM to SBOL conversion
description: chloramphenicol acetyltransferase (forwards, CmF) [cf. BBa_J31004]
created: 2006-07-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
mutable provenance: pSB1AC3
owned by: james
owned by: myers
mutable description: When a promoter and an RBS are in front of the gene, the cell will express Chloramphenicol resistance. Because it contains full biobrick ends, this part can be used to easily add chloramphenicol resistance to any part without changing plasmid vectors.
discontinued: false
dominant: true
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 918
owning group id: 61
sample status: In stock
status: status/Available
bookmark: true
mutable notes: This part is cloned into pSB1A2.
star: true
creator: Erin Zwack, Sabriya Rosemond
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CmR gene
sequence: https://synbiohub.org/public/igem/BBa_J31005_sequence/1

CmR gene
type: SequenceAnnotation
persistent identity: annotation1884999
display id: annotation1884999
version: 1
top level: CmR
location: https://synbiohub.org/public/igem/BBa_J31005/annotation1884999/range1884999/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J31005/annotation1884999/range1884999/1
type: Range
persistent identity: range1884999
display id: range1884999
version: 1
top level: CmR
direction: direction/forward
start: 1
end: 660
orientation: inline

https://synbiohub.org/public/igem/BBa_K316023_sequence/1
type: Sequence
persistent identity: BBa K316023 sequence
display id: BBa_K316023_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtttgcaaaacgattcaaaacctctttactgccgttattcgctggatttttattgctgtttcatttggttctggcaggaccggcggctgcgagtgctgaaacggcgaacaaatcgaatgagcttacagcaccgtcgatcaaaagcggaaccattcttcatgcatggaattggtcgttcaatacgttaaaacacaatatgaaggatattcatgatgcaggatatacagccattcagacatctccgattaaccaagtaaaggaagggaatcaaggagataaaagcatgtcgaactggtactggctgtatcagccgacatcgtatcaaattggcaaccgttacttaggtactgaacaagaatttaaagaaatgtgtgcagccgctgaagaatatggcataaaggtcattgttgacgcggtcatcaatcataccaccagtgattatgccgcgatttccaatgaggttaagagtattccaaactggacacatggaaacacacaaattaaaaactggtctgatcgatactagagaattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgttactagagaaaggtggtgaatactagatggagaaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatccggaatttcgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcaccatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgattcaggttcatcatgccgtttgtgatggcttccatgtcggcagaatgcttaatgaattacaacagtactgcgatgagtggcagggcggggcgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143064_sequence/1
type: Sequence
persistent identity: BBa K143064 sequence
display id: BBa_K143064_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggagaaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatccggaatttcgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcaccatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgattcaggttcatcatgccgtttgtgatggcttccatgtcggcagaatgcttaatgaattacaacagtactgcgatgagtggcagggcggggcgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143070_sequence/1
type: Sequence
persistent identity: BBa K143070 sequence
display id: BBa_K143070_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtttgcaaaacgattcaaaacctctttactgccgttattcgctggatttttattgctgtttcatttggttctggcaggaccggcggctgcgagtgctgaaacggcgaacaaatcgaatgagcttacagcaccgtcgatcaaaagcggaaccattcttcatgcatggaattggtcgttcaatacgttaaaacacaatatgaaggatattcatgatgcaggatatacagccattcagacatctccgattaaccaagtaaaggaagggaatcaaggagataaaagcatgtcgaactggtactggctgtatcagccgacatcgtatcaaattggcaaccgttacttaggtactgaacaagaatttaaagaaatgtgtgcagccgctgaagaatatggcataaaggtcattgttgacgcggtcatcaatcataccaccagtgattatgccgcgatttccaatgaggttaagagtattccaaactggacacatggaaacacacaaattaaaaactggtctgatcgatactagagaattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgttactagagaaaggtggtgaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143001_sequence/1
type: Sequence
persistent identity: BBa K143001 sequence
display id: BBa_K143001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtttgcaaaacgattcaaaacctctttactgccgttattcgctggatttttattgctgtttcatttggttctggcaggaccggcggctgcgagtgctgaaacggcgaacaaatcgaatgagcttacagcaccgtcgatcaaaagcggaaccattcttcatgcatggaattggtcgttcaatacgttaaaacacaatatgaaggatattcatgatgcaggatatacagccattcagacatctccgattaaccaagtaaaggaagggaatcaaggagataaaagcatgtcgaactggtactggctgtatcagccgacatcgtatcaaattggcaaccgttacttaggtactgaacaagaatttaaagaaatgtgtgcagccgctgaagaatatggcataaaggtcattgttgacgcggtcatcaatcataccaccagtgattatgccgcgatttccaatgaggttaagagtattccaaactggacacatggaaacacacaaattaaaaactggtctgatcga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143012_sequence/1
type: Sequence
persistent identity: BBa K143012 sequence
display id: BBa_K143012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143021_sequence/1
type: Sequence
persistent identity: BBa K143021 sequence
display id: BBa_K143021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaggtggtgaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J31005_sequence/1
type: Sequence
persistent identity: BBa J31005 sequence
display id: BBa_J31005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggagaaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatccggaatttcgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcaccatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgattcaggttcatcatgccgtttgtgatggcttccatgtcggcagaatgcttaatgaattacaacagtactgcgatgagtggcagggcggggcgtaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z