##gff-version 3
##sequence-region BBa_K316027 1 3977
BBa_K316027	.	engineered_region	654	1561	.	+	0	ID=BBa_K143065;Name=BBa_K143065
BBa_K316027	.	terminator	1433	1512	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K143065
BBa_K316027	.	stem_loop	1444	1487	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K316027	.	engineered_region	1433	1512	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K316027	.	CDS	654	1424	.	+	0	ID=BBa_K143031;Name=BBa_K143031;Parent=BBa_K143065
BBa_K316027	.	stop_codon	1422	1424	.	+	0	ID=annotation1992697;Name=stop;Parent=BBa_K143031
BBa_K316027	.	start_codon	654	656	.	+	0	ID=annotation1992698;Name=start;Parent=BBa_K143031
BBa_K316027	.	CDS	654	1418	.	+	0	ID=annotation1975961;Name=Aad9 Spectinomycin Acetyltransferase;Parent=BBa_K143031
BBa_K316027	.	stop_codon	1419	1421	.	+	0	ID=annotation1992696;Name=stop;Parent=BBa_K143031
BBa_K316027	.	terminator	1521	1561	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K143065
BBa_K316027	.	engineered_region	1521	1561	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K316027	.	stop_codon	1554	1554	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K316027	.	stem_loop	1528	1547	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K316027	.	polyA_site	1548	1561	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K316027	.	engineered_region	2924	2967	.	+	0	ID=BBa_K316014;Name=BBa_K316014
BBa_K316027	.	sequence_feature	2960	2967	.	+	0	ID=BBa_K316013;Name=BBa_K316013;Parent=BBa_K316014
BBa_K316027	.	sequence_feature	2960	2963	.	+	0	ID=annotation2098160;Name=5' product;Parent=BBa_K316013
BBa_K316027	.	sequence_feature	2964	2967	.	+	0	ID=annotation2098166;Name=3' product;Parent=BBa_K316013
BBa_K316027	.	sequence_feature	2924	2951	.	+	0	ID=BBa_K316002;Name=BBa_K316002;Parent=BBa_K316014
BBa_K316027	.	engineered_region	1570	1686	.	+	0	ID=BBa_K143053;Name=BBa_K143053
BBa_K316027	.	ribosome_entry_site	1675	1686	.	+	0	ID=BBa_K143021;Name=BBa_K143021;Parent=BBa_K143053
BBa_K316027	.	ribosome_entry_site	1675	1686	.	+	0	ID=annotation1975997;Name=rbs;Parent=BBa_K143021
BBa_K316027	.	promoter	1570	1666	.	+	0	ID=BBa_K143012;Name=BBa_K143012;Parent=BBa_K143053
BBa_K316027	.	promoter	1632	1637	.	+	0	ID=annotation1975704;Name=Sigma A-35;Parent=BBa_K143012
BBa_K316027	.	promoter	1655	1660	.	+	0	ID=annotation1975705;Name=Sigma A -10;Parent=BBa_K143012
BBa_K316027	.	engineered_region	1693	2915	.	+	0	ID=BBa_K143062;Name=BBa_K143062
BBa_K316027	.	terminator	2875	2915	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K143062
BBa_K316027	.	engineered_region	2875	2915	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K316027	.	stop_codon	2908	2908	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K316027	.	stem_loop	2882	2901	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K316027	.	polyA_site	2902	2915	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K316027	.	terminator	2787	2866	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K143062
BBa_K316027	.	stem_loop	2798	2841	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K316027	.	engineered_region	2787	2866	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K316027	.	CDS	1693	2778	.	+	0	ID=BBa_K143033;Name=BBa_K143033;Parent=BBa_K143062
BBa_K316027	.	stop_codon	2773	2775	.	+	0	ID=annotation1994271;Name=stop;Parent=BBa_K143033
BBa_K316027	.	stop_codon	2776	2778	.	+	0	ID=annotation1994272;Name=stop;Parent=BBa_K143033
BBa_K316027	.	CDS	1693	2772	.	+	0	ID=annotation1975974;Name=LacI (Lva-, N-terminal deletion) regulatory protein;Parent=BBa_K143033
BBa_K316027	.	start_codon	1693	1695	.	+	0	ID=annotation1992702;Name=start;Parent=BBa_K143033
BBa_K316027	.	sequence_feature	2976	3977	.	+	0	ID=BBa_K143002;Name=BBa_K143002
BBa_K316027	.	sequence_feature	2976	3980	.	+	0	ID=annotation1974146;Name=3' AmyE homologous sequence;Parent=BBa_K143002
BBa_K316027	.	engineered_region	531	647	.	+	0	ID=BBa_K143053;Name=BBa_K143053
BBa_K316027	.	ribosome_entry_site	636	647	.	+	0	ID=BBa_K143021;Name=BBa_K143021;Parent=BBa_K143053
BBa_K316027	.	ribosome_entry_site	636	647	.	+	0	ID=annotation1975997;Name=rbs;Parent=BBa_K143021
BBa_K316027	.	promoter	531	627	.	+	0	ID=BBa_K143012;Name=BBa_K143012;Parent=BBa_K143053
BBa_K316027	.	promoter	593	598	.	+	0	ID=annotation1975704;Name=Sigma A-35;Parent=BBa_K143012
BBa_K316027	.	promoter	616	621	.	+	0	ID=annotation1975705;Name=Sigma A -10;Parent=BBa_K143012
BBa_K316027	.	sequence_feature	1	522	.	+	0	ID=BBa_K143001;Name=BBa_K143001
BBa_K316027	.	sequence_feature	1	522	.	+	0	ID=annotation1974145;Name=5' AmyE homologous sequence;Parent=BBa_K143001
>BBa_K316027
atgtttgcaaaacgattcaaaacctctttactgccgttattcgctggatttttattgctgtttcatttggttctggcag
gaccggcggctgcgagtgctgaaacggcgaacaaatcgaatgagcttacagcaccgtcgatcaaaagcggaaccattct
tcatgcatggaattggtcgttcaatacgttaaaacacaatatgaaggatattcatgatgcaggatatacagccattcag
acatctccgattaaccaagtaaaggaagggaatcaaggagataaaagcatgtcgaactggtactggctgtatcagccga
catcgtatcaaattggcaaccgttacttaggtactgaacaagaatttaaagaaatgtgtgcagccgctgaagaatatgg
cataaaggtcattgttgacgcggtcatcaatcataccaccagtgattatgccgcgatttccaatgaggttaagagtatt
ccaaactggacacatggaaacacacaaattaaaaactggtctgatcgatactagagaattttgtcaaaataattttatt
gacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgttacta
gagaaaggtggtgaatactagatgaggaggatatatttgaatacatacgaacaaattaataaagtgaaaaaaatacttc
ggaaacatttaaaaaataaccttattggtacttacatgtttggatcaggagttgagagtggactaaaaccaaatagtga
tcttgactttttagtcgtcgtatctgaaccattgacagatcaaagtaaagaaatacttatacaaaaaattagacctatt
tcaaaaaaaataggagataaaagcaacttacgatatattgaattaacaattattattcagcaagaaatggtaccgtgga
atcatcctcccaaacaagaatttatttatggagaatggttacaagagctttatgaacaaggatacattcctcagaagga
attaaattcagatttaaccataatgctttaccaagcaaaacgaaaaaataaaagaatatacggaaattatgacttagag
gaattactacctgatattccattttctgatgtgagaagagccattatggattcgtcagaggaattaatagataattatc
aggatgatgaaaccaactctatattaactttatgccgtatgattttaactatggacacgggtaaaatcataccaaaaga
tattgcgggaaatgcagtggctgaatcttctccattagaacatagggagagaattttgttagcagttcgtagttatctt
ggagagaatattgaatggactaatgaaaatgtaaatttaactataaactatttaaataacagattaaaaaaattataat
aatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcgg
tgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattttgtcaa
aataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaa
taaatgttactagagaaaggtggtgaatactagatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtct
cttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgat
ggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacc
tccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtgg
tggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaaacggcggtgcacaatcttctcgcgcaacgcgtcagtgg
gctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttattt
cttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatc
tggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctgg
ctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggtttt
caacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcg
caatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacag
ctcatgttatatcccgccgtcaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctg
caactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgc
ccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaag
cgggcagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagag
atctcctagaatatatattatgtaaacttactagaggaaatttctactagagatccgtttaggctgggcggtgatagct
tctcgttcaggcagtacgcctcttttcttttccagacctgagggaggcggaaatggtgtgaggttcccggggaaaagcc
aaataggcgatcgcgggagtgctttatttgaagatcaggctatcactgcggtcaatagatttcacaatgtgatggctgg
acagcctgaggaactctcgaacccgaatggaaacaaccagatatttatgaatcagcgcggctcacatggcgttgtgctg
gcaaatgcaggttcatcctctgtctctatcaatacggcaacaaaattgcctgatggcaggtatgacaataaagctggag
cgggttcatttcaagtgaacgatggtaaactgacaggcacgatcaatgccaggtctgtagctgtgctttatcctgatga
tattgcaaaagcgcctcatgttttccttgagaattacaaaacaggtgtaacacattctttcaatgatcaactgacgatt
accttgcgtgcagatgcgaatacaacaaaagccgtttatcaaatcaataatggaccagagacggcgtttaaggatggag
atcaattcacaatcggaaaaggagatccatttggcaaaacatacaccatcatgttaaaaggaacgaacagtgatggtgt
aacgaggaccgagaaatacagttttgttaaaagagatccagcgtcggccaaaaccatcggctatcaaaatccgaatcat
tggagccaggtaaatgcttatatctataaacatgatgggagccgagtaattgaattgaccggatcttggcctggaaaac
caatgactaaaaatgcagacggaatttacacgctgacgctgcctgcggacacggatacaaccaacgcaaaagtgatttt
taataatggcagcgcccaagtgcccggtcagaatcagcctggctttgattacgtgctaaatggtttatataatgactcg
ggcttaagcggttctcttccccattga