BBa_K316027
type: ComponentDefinition
persistent identity: BBa K316027
display id: BBa_K316027
version: 1
was generated by: iGEM to SBOL conversion
description: B.subtilis transformation vector with LacI
created: 2010-10-25T11:00:00Z
modified: 2015-05-08T01:11:56Z
mutable provenance: Existing biobricks, <bbpart>BBa_K143070</bbpart>, <bbpart>BBa_K316002</bbpart>, <bbpart>BBa_K316014</bbpart> <bbpart>BBa_K143002</bbpart>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: yey
discontinued: false
dominant: false
experience: experience/Works
group u list: _440_
m user id: 0
owner id: 7480
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: This part is designed for integration into ''B. subtilis' genome at amyE locus, it contains Spectinomycin resistance selection marker and constitutively expresses LacI. PmeI can be used to ligate a gene of interest using blunt ended methods.
star: false
creator: IC 2010 Team
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K143002
component: BBa_K316014
component: BBa_K143001
component: BBa_K143062
component: BBa_K143053
component: BBa_K143065
sequence annotation: BBa_K143001
sequence annotation: BBa_K143053
sequence annotation: BBa_K143065
sequence annotation: BBa_K143062
sequence annotation: BBa_K316014
sequence annotation: BBa_K143002
sequence: https://synbiohub.org/public/igem/BBa_K316027_sequence/1

BBa_K143002
type: Component
persistent identity: component2102373
display id: component2102373
version: 1
top level: BBa_K316027
definition: amyE 3 IS
access: public

BBa_K316014
type: Component
persistent identity: component2102371
display id: component2102371
version: 1
top level: BBa_K316027
definition: BBa_K316014
access: public

BBa_K143001
type: Component
persistent identity: component2102333
display id: component2102333
version: 1
top level: BBa_K316027
definition: amyE 5 IS
access: public

BBa_K143062
type: Component
persistent identity: component2102366
display id: component2102366
version: 1
top level: BBa_K316027
definition: LacI-T
access: public

BBa_K143053
type: Component
persistent identity: component2102354
display id: component2102354
version: 1
top level: BBa_K316027
definition: Pveg-spoVG
access: public

BBa_K143065
type: Component
persistent identity: component2102348
display id: component2102348
version: 1
top level: BBa_K316027
definition: Aad9-T
access: public

BBa_K143053
type: Component
persistent identity: component2102339
display id: component2102339
version: 1
top level: BBa_K316027
definition: Pveg-spoVG
access: public

BBa_K143001
type: SequenceAnnotation
persistent identity: annotation2102333
display id: annotation2102333
version: 1
top level: BBa_K316027
location: https://synbiohub.org/public/igem/BBa_K316027/annotation2102333/range2102333/1
component: BBa_K143001

https://synbiohub.org/public/igem/BBa_K316027/annotation2102333/range2102333/1
type: Range
persistent identity: range2102333
display id: range2102333
version: 1
top level: BBa_K316027
direction: direction/reverse
start: 1
end: 522
orientation: inline

BBa_K143053
type: SequenceAnnotation
persistent identity: annotation2102339
display id: annotation2102339
version: 1
top level: BBa_K316027
location: https://synbiohub.org/public/igem/BBa_K316027/annotation2102339/range2102339/1
component: BBa_K143053

https://synbiohub.org/public/igem/BBa_K316027/annotation2102339/range2102339/1
type: Range
persistent identity: range2102339
display id: range2102339
version: 1
top level: BBa_K316027
direction: direction/reverse
start: 531
end: 647
orientation: inline

BBa_K143065
type: SequenceAnnotation
persistent identity: annotation2102348
display id: annotation2102348
version: 1
top level: BBa_K316027
location: https://synbiohub.org/public/igem/BBa_K316027/annotation2102348/range2102348/1
component: BBa_K143065

https://synbiohub.org/public/igem/BBa_K316027/annotation2102348/range2102348/1
type: Range
persistent identity: range2102348
display id: range2102348
version: 1
top level: BBa_K316027
direction: direction/reverse
start: 654
end: 1561
orientation: inline

BBa_K143053
type: SequenceAnnotation
persistent identity: annotation2102354
display id: annotation2102354
version: 1
top level: BBa_K316027
location: https://synbiohub.org/public/igem/BBa_K316027/annotation2102354/range2102354/1
component: BBa_K143053

https://synbiohub.org/public/igem/BBa_K316027/annotation2102354/range2102354/1
type: Range
persistent identity: range2102354
display id: range2102354
version: 1
top level: BBa_K316027
direction: direction/reverse
start: 1570
end: 1686
orientation: inline

BBa_K143062
type: SequenceAnnotation
persistent identity: annotation2102366
display id: annotation2102366
version: 1
top level: BBa_K316027
location: https://synbiohub.org/public/igem/BBa_K316027/annotation2102366/range2102366/1
component: BBa_K143062

https://synbiohub.org/public/igem/BBa_K316027/annotation2102366/range2102366/1
type: Range
persistent identity: range2102366
display id: range2102366
version: 1
top level: BBa_K316027
direction: direction/reverse
start: 1693
end: 2915
orientation: inline

BBa_K316014
type: SequenceAnnotation
persistent identity: annotation2102371
display id: annotation2102371
version: 1
top level: BBa_K316027
location: https://synbiohub.org/public/igem/BBa_K316027/annotation2102371/range2102371/1
component: BBa_K316014

https://synbiohub.org/public/igem/BBa_K316027/annotation2102371/range2102371/1
type: Range
persistent identity: range2102371
display id: range2102371
version: 1
top level: BBa_K316027
direction: direction/reverse
start: 2924
end: 2967
orientation: inline

BBa_K143002
type: SequenceAnnotation
persistent identity: annotation2102373
display id: annotation2102373
version: 1
top level: BBa_K316027
location: https://synbiohub.org/public/igem/BBa_K316027/annotation2102373/range2102373/1
component: BBa_K143002

https://synbiohub.org/public/igem/BBa_K316027/annotation2102373/range2102373/1
type: Range
persistent identity: range2102373
display id: range2102373
version: 1
top level: BBa_K316027
direction: direction/reverse
start: 2976
end: 3977
orientation: inline

amyE 5 IS
type: ComponentDefinition
persistent identity: BBa K143001
display id: BBa_K143001
version: 1
was generated by: iGEM to SBOL conversion
description: 5??? Integration Sequence for the amyE locus of B. subtilis
created: 2008-08-26T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The 5??? integration sequence was taken from the shuttle vector pDR111 which has been used in many studies on ''B.subtilis'', in particular in the studies of transcriptional control#1 #2 #3 <biblio> #1 pmid=14597697 #2 pmid=15937167 #3 pmid=12169614 </biblio>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The 5' integration sequence can be added to the front of a Biobrick construct and the 3' integration sequence specific for this locus (Part BBa_K143002) to the rear of the Biobrick construct to allow integration of the Biobrick construct into the chromosome of the gram positive bacterium B.subtilis. The AmyE locus was the first locus used for integration into ''B.subtilis'' by Shimotsu and Henner#1 and is still commonly used in vectors such as pDR111#2, pDL#3 and their derivatives. Integration at the AmyE locus removes the ability of ''B.subtilis'' to break down starch, which can be assayed with iodine as described by Cutting and Vander-horn#4. The 5' and 3' integration sequences for the AmyE locus were used to integrate the Imperial 2008 iGEM project primary construct into the ''B.sutbilis'' chromosome. <biblio> #1 pmid=3019840 #2 pmid=14597697 #3 ''Bacillus'' Genetic Stock Center [www.bgsc.org] #4 Cutting, S M.; Vander-Horn, P B. Genetic analysis. In: Harwood C R, Cutting S M. , editors. Molecular biological methods for Bacillus. Chichester, England: John Wiley & Sons, Ltd.; 1990. pp. 27???74. </biblio>
discontinued: false
dominant: false
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: The AmyE integration sequence was taken from the vector after comparison by BLAST to the ''B.subtilis'' chromosome to identify the homologous sequences. The sequence present in both the host chromosome and the plasmid at the 5' end of the gene is the 5' sequence required for integration.
star: true
creator: Chris Hirst
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: 5' AmyE homologous sequence
sequence: https://synbiohub.org/public/igem/BBa_K143001_sequence/1

5' AmyE homologous sequence
type: SequenceAnnotation
persistent identity: annotation1974145
display id: annotation1974145
version: 1
top level: amyE 5 IS
location: https://synbiohub.org/public/igem/BBa_K143001/annotation1974145/range1974145/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K143001/annotation1974145/range1974145/1
type: Range
persistent identity: range1974145
display id: range1974145
version: 1
top level: amyE 5 IS
direction: direction/forward
start: 1
end: 522
orientation: inline

amyE 3 IS
type: ComponentDefinition
persistent identity: BBa K143002
display id: BBa_K143002
version: 1
was generated by: iGEM to SBOL conversion
description: 3??? Integration Sequence for the amyE locus of B. subtilis
created: 2008-08-27T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The 3??? integration sequence was taken from the shuttle vector pDR111 which has been used in many studies on B.subtilis, in particular in the studies of transcriptional control[1,2,3] References 1.Shimotsu H and Henner DJ. Construction of a single-copy integration vector and its use in analysis of regulation of the trp operon of Bacillus subtilis. Gene 1986; 43(1-2) 85-94. pmid:3019840. 2.Erwin KN, Nakano S, and Zuber P. Sulfate-dependent repression of genes that function in organosulfur metabolism in Bacillus subtilis requires Spx. J Bacteriol 2005 Jun; 187(12) 4042-9. doi:10.1128/JB.187.12.4042-4049.2005 pmid:15937167 3.Britton RA, Eichenberger P, Gonzalez-Pastor JE, Fawcett P, Monson R, Losick R, and Grossman AD. Genome-wide analysis of the stationary-phase sigma factor (sigma-H) regulon of Bacillus subtilis. J Bacteriol 2002 Sep; 184(17) 4881-90. pmid:12169614
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Integration sequences allow DNA to be incorporated into the chromosome of a host cell at a specific locus using leading (5') and trailing (3') DNA sequences that are the same as those at a specific locus of the chromosome. The 5' integration sequence can be added to the front of a Biobrick construct and the 3' integration sequence specific for this locus (Part BBa_K143002) to the rear of the Biobrick construct to allow integration of the Biobrick construct into the chromosome of the gram positive bacterium B.subtilis. The AmyE locus was the first locus used for integration into B.subtilis by Shimotsu and Henner[1] and is still commonly used in vectors such as pDR111[2], pDL[3] and their derivatives. Integration at the AmyE locus removes the ability of B.subtilis to break down starch, which can be assayed with iodine as described by Cutting and Vander-horn[4]. The 5' and 3' integration sequences for the AmyE locus were used to integrate the Imperial 2008 iGEM project primary construct into the B.sutbilis chromosome. References 1. Shimotsu H and Henner DJ. Construction of a single-copy integration vector and its use in analysis of regulation of the trp operon of Bacillus subtilis. Gene 1986; 43(1-2) 85-94. pmid:3019840 2.Nakano S, Kster-Schck E, Grossman AD, and Zuber P. Spx-dependent global transcriptional control is induced by thiol-specific oxidative stress in Bacillus subtilis. Proc Natl Acad Sci U S A 2003 Nov 11; 100(23) 13603-8. doi:10.1073/pnas.2235180100 pmid:14597697 3.Bacillus Genetic Stock Center; www.bgsc.org 4.Cutting, S M.; Vander-Horn, P B. Genetic analysis. In: Harwood C R, Cutting S M. , editors. Molecular biological methods for Bacillus. Chichester, England: John Wiley & Sons, Ltd.; 1990. pp. 27???74.
discontinued: false
dominant: false
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: The AmyE integration sequence was taken from the vector after comparison by BLAST to the B.subtilis chromosome to identify the homologous sequences. The sequence present in both the host chromosome and the plasmid at the 3' end of the gene is the 3' sequence required for integration
star: true
creator: Chris Hirst
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: 3' AmyE homologous sequence
sequence: https://synbiohub.org/public/igem/BBa_K143002_sequence/1

3' AmyE homologous sequence
type: SequenceAnnotation
persistent identity: annotation1974146
display id: annotation1974146
version: 1
top level: amyE 3 IS
location: https://synbiohub.org/public/igem/BBa_K143002/annotation1974146/range1974146/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K143002/annotation1974146/range1974146/1
type: Range
persistent identity: range1974146
display id: range1974146
version: 1
top level: amyE 3 IS
direction: direction/forward
start: 1
end: 1005
orientation: inline

LacI-T
type: ComponentDefinition
persistent identity: BBa K143062
display id: BBa_K143062
version: 1
was generated by: iGEM to SBOL conversion
description: LacI repressor protein - Terminator
created: 2008-09-30T11:00:00Z
modified: 2015-05-08T01:10:24Z
mutable provenance: LacI was produced by PCR cloning using Pfu form the ''B. subtilis'' integration vector and cloned into a BioBrick with the registry double terminator.
owned by: james
owned by: myers
mutable description: LacI transcriptional repressor protein (<bbpart>BBa_K143033</bbpart>) coupled to the double terminator (<bbpart>BBa_B0015</bbpart>. The LacI does not possess a LVA degradation tag and gas a short (3 amino acid) N-terminal deletion consistent with LacI used in conjunction with ''B. subtilis''. LacI can be used in conjunction with the lac operon promoter (<bbpart>BBa_K143015</bbpart>), where the LacI will act as a receiver for an IPTG input to result in a '''Polymerases per second''' (PoPS) output. The double terminator is the most commonly used terminator and is a combination of parts <bbpart>BBa_B0010</bbpart> and <bbpart>BBa_B0012</bbpart>. The double terminator allows the LacI to be easily incorporated into a closed transcriptional unit.
discontinued: false
dominant: false
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: LacI was identified from the pDR111 ''B. subtilis'' integration vector. The double terminator is the most commonly used registry terminator.
star: false
creator: Chris Hirst
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K143033
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_K143033
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K143062_sequence/1

BBa_K143033
type: Component
persistent identity: component1994509
display id: component1994509
version: 1
top level: LacI-T
definition: LacI
access: public

BBa_B0012
type: Component
persistent identity: component1994512
display id: component1994512
version: 1
top level: LacI-T
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1994510
display id: component1994510
version: 1
top level: LacI-T
definition: BBa_B0010
access: public

BBa_K143033
type: SequenceAnnotation
persistent identity: annotation1994509
display id: annotation1994509
version: 1
top level: LacI-T
location: https://synbiohub.org/public/igem/BBa_K143062/annotation1994509/range1994509/1
component: BBa_K143033

https://synbiohub.org/public/igem/BBa_K143062/annotation1994509/range1994509/1
type: Range
persistent identity: range1994509
display id: range1994509
version: 1
top level: LacI-T
direction: direction/reverse
start: 1
end: 1086
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1994510
display id: annotation1994510
version: 1
top level: LacI-T
location: https://synbiohub.org/public/igem/BBa_K143062/annotation1994510/range1994510/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K143062/annotation1994510/range1994510/1
type: Range
persistent identity: range1994510
display id: range1994510
version: 1
top level: LacI-T
direction: direction/forward
start: 1095
end: 1174
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1994512
display id: annotation1994512
version: 1
top level: LacI-T
location: https://synbiohub.org/public/igem/BBa_K143062/annotation1994512/range1994512/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K143062/annotation1994512/range1994512/1
type: Range
persistent identity: range1994512
display id: range1994512
version: 1
top level: LacI-T
direction: direction/forward
start: 1183
end: 1223
orientation: inline

Pveg-spoVG
type: ComponentDefinition
persistent identity: BBa K143053
display id: BBa_K143053
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter Pveg and RBS spoVG for B. subtilis
created: 2008-10-07T11:00:00Z
modified: 2015-05-08T01:10:24Z
mutable provenance: Pveg-spoVG was synthesised by GeneArt
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Constitutive promoter veg(<bbpart>BBa_K143012</bbpart>) coupled to the strong Ribosome Binding Site spoVG(<bbpart>BBa_K143021</bbpart>) from ''B. subtilis''. Pveg-spoVG can be used in the context of a '''Ribosomes per second''' (RiPS) output generator '''To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix1. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.'''
discontinued: false
dominant: true
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The sequence of Pveg was obtained from the DBTBS1 and RBS-spoVG were obtained from papers2 and the sequence synthesised by GeneArt
star: true
creator: Chris Hirst
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K143012
component: BBa_K143021
sequence annotation: BBa_K143012
sequence annotation: BBa_K143021
sequence: https://synbiohub.org/public/igem/BBa_K143053_sequence/1

BBa_K143012
type: Component
persistent identity: component1979395
display id: component1979395
version: 1
top level: Pveg-spoVG
definition: Pveg
access: public

BBa_K143021
type: Component
persistent identity: component1979397
display id: component1979397
version: 1
top level: Pveg-spoVG
definition: RBS-spoVG
access: public

BBa_K143012
type: SequenceAnnotation
persistent identity: annotation1979395
display id: annotation1979395
version: 1
top level: Pveg-spoVG
location: https://synbiohub.org/public/igem/BBa_K143053/annotation1979395/range1979395/1
component: BBa_K143012

https://synbiohub.org/public/igem/BBa_K143053/annotation1979395/range1979395/1
type: Range
persistent identity: range1979395
display id: range1979395
version: 1
top level: Pveg-spoVG
direction: direction/reverse
start: 1
end: 97
orientation: inline

BBa_K143021
type: SequenceAnnotation
persistent identity: annotation1979397
display id: annotation1979397
version: 1
top level: Pveg-spoVG
location: https://synbiohub.org/public/igem/BBa_K143053/annotation1979397/range1979397/1
component: BBa_K143021

https://synbiohub.org/public/igem/BBa_K143053/annotation1979397/range1979397/1
type: Range
persistent identity: range1979397
display id: range1979397
version: 1
top level: Pveg-spoVG
direction: direction/reverse
start: 106
end: 117
orientation: inline

Aad9-T
type: ComponentDefinition
persistent identity: BBa K143065
display id: BBa_K143065
version: 1
was generated by: iGEM to SBOL conversion
description: Spectinomycin Resistance Protein (Aad9) - Terminator
created: 2008-10-08T11:00:00Z
modified: 2015-05-08T01:10:24Z
mutable provenance: Aad9 was PCR cloned from the ''B. subtilis'' integration vector utilising Pfu DNA polymerase and cloned into a BioBrick iwth the double terminator was taken from the registry
owned by: james
owned by: myers
mutable description: Aad9 spectinomycin resistance protein(<bbpart>BBa_K143031</bbpart>) coupled to the double terminator (<bbpart>BBa_B0015</bbpart>). Aad9 confers resistance to spectinomycin. The double terminator is the most commonly used terminator and is a combination of parts <bbpart>BBa_B0010</bbpart> and <bbpart>BBa_B0012</bbpart>. The double terminator allows the Spectinomycin resistance gene to be incorporated into a closed transcriptional unit.
discontinued: false
dominant: false
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Aad9 was PCR cloned from the ''B. subtilis'' integration vector utilising Pfu DNA polymerase. The double terminator is the most commonly used registry termiantor.
star: false
creator: Chris Hirst and Imperial iGEM 08
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K143031
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_K143031
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K143065_sequence/1

BBa_K143031
type: Component
persistent identity: component1985265
display id: component1985265
version: 1
top level: Aad9-T
definition: Aad9
access: public

BBa_B0012
type: Component
persistent identity: component1985268
display id: component1985268
version: 1
top level: Aad9-T
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1985266
display id: component1985266
version: 1
top level: Aad9-T
definition: BBa_B0010
access: public

BBa_K143031
type: SequenceAnnotation
persistent identity: annotation1985265
display id: annotation1985265
version: 1
top level: Aad9-T
location: https://synbiohub.org/public/igem/BBa_K143065/annotation1985265/range1985265/1
component: BBa_K143031

https://synbiohub.org/public/igem/BBa_K143065/annotation1985265/range1985265/1
type: Range
persistent identity: range1985265
display id: range1985265
version: 1
top level: Aad9-T
direction: direction/reverse
start: 1
end: 771
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1985266
display id: annotation1985266
version: 1
top level: Aad9-T
location: https://synbiohub.org/public/igem/BBa_K143065/annotation1985266/range1985266/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K143065/annotation1985266/range1985266/1
type: Range
persistent identity: range1985266
display id: range1985266
version: 1
top level: Aad9-T
direction: direction/forward
start: 780
end: 859
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1985268
display id: annotation1985268
version: 1
top level: Aad9-T
location: https://synbiohub.org/public/igem/BBa_K143065/annotation1985268/range1985268/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K143065/annotation1985268/range1985268/1
type: Range
persistent identity: range1985268
display id: range1985268
version: 1
top level: Aad9-T
direction: direction/forward
start: 868
end: 908
orientation: inline

BBa_K316014
type: ComponentDefinition
persistent identity: BBa K316014
display id: BBa_K316014
version: 1
was generated by: iGEM to SBOL conversion
description: Dif sequence followed by PmeI recognition site
created: 2010-10-22T11:00:00Z
modified: 2015-05-08T01:11:56Z
mutable provenance: Oligonucleotide synthesis of single stranded primers.
owned by: james
owned by: myers
mutable description: This composite part of <bbpart>BBa_K143000</bbpart> and <bbpart>BBa_K143013</bbpart>. The dif site can be used in conjunction with another dif site in another part of the vector to remove a sequence between the two dif sites. PmeI site can be used for blunt end cloning of a DNA sequence behind the dif site. Please see ???Part Design??? section for design considerations and parts used.
discontinued: false
dominant: false
experience: experience/None
group u list: _440_
m user id: 0
owner id: 7480
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: This part was designed to be cloned using standard biobrick methods. Two single stranded, synthetic oligos were annealed to produce a double stranded DNA sequence with single stranded overhangs identical to the product of digestion by EcoRI and SpeI. Thus compatible with biobrick cloning methods.
star: false
creator: IC 2010 Team
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_K316013
component: BBa_K316002
sequence annotation: BBa_K316002
sequence annotation: BBa_K316013
sequence: https://synbiohub.org/public/igem/BBa_K316014_sequence/1

BBa_K316013
type: Component
persistent identity: component2098249
display id: component2098249
version: 1
top level: BBa_K316014
definition: PmeI site
access: public

BBa_K316002
type: Component
persistent identity: component2098246
display id: component2098246
version: 1
top level: BBa_K316014
definition: Bs dif
access: public

BBa_K316002
type: SequenceAnnotation
persistent identity: annotation2098246
display id: annotation2098246
version: 1
top level: BBa_K316014
location: https://synbiohub.org/public/igem/BBa_K316014/annotation2098246/range2098246/1
component: BBa_K316002

https://synbiohub.org/public/igem/BBa_K316014/annotation2098246/range2098246/1
type: Range
persistent identity: range2098246
display id: range2098246
version: 1
top level: BBa_K316014
direction: direction/reverse
start: 1
end: 28
orientation: inline

BBa_K316013
type: SequenceAnnotation
persistent identity: annotation2098249
display id: annotation2098249
version: 1
top level: BBa_K316014
location: https://synbiohub.org/public/igem/BBa_K316014/annotation2098249/range2098249/1
component: BBa_K316013

https://synbiohub.org/public/igem/BBa_K316014/annotation2098249/range2098249/1
type: Range
persistent identity: range2098249
display id: range2098249
version: 1
top level: BBa_K316014
direction: direction/reverse
start: 37
end: 44
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

Pveg
type: ComponentDefinition
persistent identity: BBa K143012
display id: BBa_K143012
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter veg a constitutive promoter for B. subtilis
created: 2008-09-10T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The Pveg promoter was suggested to us by Dr. Jan-Willem Veening of Newcastle University. This sequence supplied was compared to that of the DBTBS database#3 then a section containing the binding site synthesised by Geneart.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Pveg is a constitutive promoter that constitutively expresses the P43 protein in ''B.subtilis''. Pveg contains binding sites for the ''B.sutbilis'' major sigma factor#1. Pveg in ''B.subtilis'' utilises two binding sites to cause high expression of genes#2, however our Pveg is lacking the upstream site to give a medium level of gene expression. It has been noted that the sporulation master regulatoion factor spoOA interacts with Pveg though it is not known how#3. The context with which we used the promoter Pveg is as a '''Polymerase Per Second''' (PoPS) generator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The biobrick part was designed to include a single binding site for the ''B.subtilis major sigma factor. In addition the biobrick standard was applied to the promoter Pveg sequence.
star: false
creator: James Chappell
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: Sigma A-35
sequence annotation: Sigma A -10
sequence: https://synbiohub.org/public/igem/BBa_K143012_sequence/1

Sigma A-35
type: SequenceAnnotation
persistent identity: annotation1975704
display id: annotation1975704
version: 1
top level: Pveg
location: https://synbiohub.org/public/igem/BBa_K143012/annotation1975704/range1975704/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K143012/annotation1975704/range1975704/1
type: Range
persistent identity: range1975704
display id: range1975704
version: 1
top level: Pveg
direction: direction/forward
start: 63
end: 68
orientation: inline

Sigma A -10
type: SequenceAnnotation
persistent identity: annotation1975705
display id: annotation1975705
version: 1
top level: Pveg
location: https://synbiohub.org/public/igem/BBa_K143012/annotation1975705/range1975705/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K143012/annotation1975705/range1975705/1
type: Range
persistent identity: range1975705
display id: range1975705
version: 1
top level: Pveg
direction: direction/forward
start: 86
end: 91
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa K143033
display id: BBa_K143033
version: 1
was generated by: iGEM to SBOL conversion
description: LacI (Lva-, N-terminal deletion) regulatory protein
created: 2008-09-15T11:00:00Z
modified: 2015-05-08T01:10:24Z
mutable provenance: The LacI gene was cloned from''B. subtilis'' shuttle vector pDR111 using Pfu DNA polymerase PCR
owned by: james
owned by: myers
mutable description: LacI is a regulatory protein responsible for the repression of many catabolite genes. Transcription is regulated by proteins which bind operator sequences around the transcription start site. These proteins can positively affect transcription (activators) or negatively affect transcription (reppresors). Some repressor proteins can be inactivted however by addition of an inducer, such as IPTG or certain sugars. LacI if the regulator protein for the lactose operon in ''E.coli'' and the hyper-spank protein of ''B. subtilis''#1(<bbpart>BBaK143015</bbpart>) and is responsible for ensuring that in the absence of lactose (or IPTG) that there is no expression trough these promoter. LacI is not endogenous to ''B. subtilis'', so LacI will need to be expressed in the host in order for the hyper-spank promoter to be regulated. In the presence of IPTG or lactose, the LacI tetramer is unable to bind DNA and so transcription resumes. This version of LacI lacks a Lva degradation tag and has a small(3 amino acid) N-terminal deletion relative to the current registry LacI (<bbpart>BBa_C0012</bbpart)> and is derivatives. The N-terminal deletion appears to be common to most of the LacI genes used in conjunction with ''B. subtilis'' though both forms are found in ''E.coli'' (in differing strains). LacI was used in conjunction with the '''Hyper-spank promoter''' (<bbpart>BBa_K143015<bbpart>) and acted as an input adaptor for a '''Polymerases per second''' (POPS) output ====References==== <biblio> #1 pmid=16166525 </biblio>
discontinued: false
dominant: false
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: LacI was located in the sequence of the ''B. subtilis'' shuttle vector pDR111. This version of LacI lacks a Ltva degradation sequence and has a small N-terminal deletion that is observed in many LacI used in studies on ''B.subtilis''. In particular, this LacI protein is used in pDR111 to regulate expression of the inducible Phyper-spank protein (<bbpart>BBa_K143015</bbpart>) (also used in the pDR111 vector). The BioBrick prefix and suffix were applied to the gene
star: false
creator: Chris Hirst
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: LacI (Lva-, N-terminal deletion) regulatory protein
sequence annotation: start
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K143033_sequence/1

LacI (Lva-, N-terminal deletion) regulatory protein
type: SequenceAnnotation
persistent identity: annotation1975974
display id: annotation1975974
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_K143033/annotation1975974/range1975974/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K143033/annotation1975974/range1975974/1
type: Range
persistent identity: range1975974
display id: range1975974
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 1080
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1992702
display id: annotation1992702
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_K143033/annotation1992702/range1992702/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K143033/annotation1992702/range1992702/1
type: Range
persistent identity: range1992702
display id: range1992702
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1994271
display id: annotation1994271
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_K143033/annotation1994271/range1994271/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K143033/annotation1994271/range1994271/1
type: Range
persistent identity: range1994271
display id: range1994271
version: 1
top level: LacI
direction: direction/forward
start: 1081
end: 1083
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1994272
display id: annotation1994272
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_K143033/annotation1994272/range1994272/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K143033/annotation1994272/range1994272/1
type: Range
persistent identity: range1994272
display id: range1994272
version: 1
top level: LacI
direction: direction/forward
start: 1084
end: 1086
orientation: inline

Aad9
type: ComponentDefinition
persistent identity: BBa K143031
display id: BBa_K143031
version: 1
was generated by: iGEM to SBOL conversion
description: Aad9 Spectinomycin Resistance Gene
created: 2008-09-15T11:00:00Z
modified: 2015-05-08T01:10:24Z
mutable provenance: Aad9 was PCR cloned from the ''B. subtilis'' integration vector pDR111 using Pfu DNA polymerase
owned by: james
owned by: myers
mutable description: Aad9 is the spectinomycin resistance gene from ''Enterococcus faecalis''#1. Expression in a host confers resistance to spectinomycin at a concentration of 100μg/μl and has been used in a variety of vectors for both ''B. subtilis'' and ''E. coli'' including pDP870#2, pCOMT-Kan#3 and pIEF16s#4 ====References==== <biblio> #1 pmid=1659306 #2 pmid=16997985 #3 pmid=15060042 #4 pmid=16714443 </biblio>
discontinued: false
dominant: false
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The sequence of ''B. subtilis'' integration vector pDR111 was searched for the spectinomycin resistance gene and the Biobrick standard applied to the gene sequence upon PCR cloning
star: false
creator: Chris Hirst
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Aad9 Spectinomycin Acetyltransferase
sequence annotation: start
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K143031_sequence/1

Aad9 Spectinomycin Acetyltransferase
type: SequenceAnnotation
persistent identity: annotation1975961
display id: annotation1975961
version: 1
top level: Aad9
location: https://synbiohub.org/public/igem/BBa_K143031/annotation1975961/range1975961/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K143031/annotation1975961/range1975961/1
type: Range
persistent identity: range1975961
display id: range1975961
version: 1
top level: Aad9
direction: direction/forward
start: 1
end: 765
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1992698
display id: annotation1992698
version: 1
top level: Aad9
location: https://synbiohub.org/public/igem/BBa_K143031/annotation1992698/range1992698/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K143031/annotation1992698/range1992698/1
type: Range
persistent identity: range1992698
display id: range1992698
version: 1
top level: Aad9
direction: direction/forward
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1992696
display id: annotation1992696
version: 1
top level: Aad9
location: https://synbiohub.org/public/igem/BBa_K143031/annotation1992696/range1992696/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K143031/annotation1992696/range1992696/1
type: Range
persistent identity: range1992696
display id: range1992696
version: 1
top level: Aad9
direction: direction/forward
start: 766
end: 768
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1992697
display id: annotation1992697
version: 1
top level: Aad9
location: https://synbiohub.org/public/igem/BBa_K143031/annotation1992697/range1992697/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K143031/annotation1992697/range1992697/1
type: Range
persistent identity: range1992697
display id: range1992697
version: 1
top level: Aad9
direction: direction/forward
start: 769
end: 771
orientation: inline

RBS-spoVG
type: ComponentDefinition
persistent identity: BBa K143021
display id: BBa_K143021
version: 1
was generated by: iGEM to SBOL conversion
description: SpoVG ribosome binding site (RBS) for B. subtilis
created: 2008-09-16T11:00:00Z
modified: 2015-05-08T01:10:23Z
mutable provenance: The sequence was taken from a previous research paper [1] and was constructed by Geneart.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Description: SpoVG is an endogenous ribosome binding site from B.subtilis. The sequence of the spoVG ribosome binding site is AAAGGUGGUGA which is complementary to the sequence UUUCCUCCACU from the 3' region of the 16s rRNA from B.subtilis. Previous research showed that the predicted binding energy of the 16s rRNA to the RBS is -19kcal 1
discontinued: false
dominant: true
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 2090
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In order to ensure that the RBS is functional the actual ribosome binding site was maintained and the distance between the RBS and the start codon maintained. In order to conform to the biobrick standard the sequence flanking the RBS had to be changed but the distance between the promoter and RBS, and start codon and RBS was maintained.
star: false
creator: James Chappell
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K143021_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1975997
display id: annotation1975997
version: 1
top level: RBS-spoVG
location: https://synbiohub.org/public/igem/BBa_K143021/annotation1975997/range1975997/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K143021/annotation1975997/range1975997/1
type: Range
persistent identity: range1975997
display id: range1975997
version: 1
top level: RBS-spoVG
direction: direction/forward
start: 1
end: 12
orientation: inline

Bs dif
type: ComponentDefinition
persistent identity: BBa K316002
display id: BBa_K316002
version: 1
was generated by: iGEM to SBOL conversion
description: dif excision site from B. subtilis
created: 2010-10-19T11:00:00Z
modified: 2015-05-08T01:11:56Z
mutable provenance: The dif sites were made by annealing synthestised oligoes.
owned by: james
owned by: myers
mutable description: Dif sites are naturally found in B.subtilis and are used by this organism during genome replication.
discontinued: false
dominant: false
experience: experience/None
group u list: _440_
m user id: 0
owner id: 7480
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The dif site was made by oligos designed to make overhangs for EcoRI and SpeI ( and ) or XbaI and PstI ( and ) to be used in standard Biobrick or 3A cloning.
star: false
creator: IC 2010 Team
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K316002_sequence/1

PmeI site
type: ComponentDefinition
persistent identity: BBa K316013
display id: BBa_K316013
version: 1
was generated by: iGEM to SBOL conversion
description: 8bp recognition sequence for PmeI restriction endonuclease
created: 2010-10-22T11:00:00Z
modified: 2015-05-08T01:11:56Z
mutable provenance: This is a planning part
owned by: james
owned by: myers
mutable description: Information about PmeI restriction endonuclease is available at [http://www.neb.com/nebecomm/products/productR0560.asp]. The recognition site is a 8bp sequence GTTTAAAC. Pme produces a blunt cut after GTTT.
discontinued: false
dominant: false
experience: experience/None
group u list: _440_
m user id: 0
owner id: 7480
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This is a planning part
star: false
creator: IC 2010 Team
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: 5' product
sequence annotation: 3' product
sequence: https://synbiohub.org/public/igem/BBa_K316013_sequence/1

5' product
type: SequenceAnnotation
persistent identity: annotation2098160
display id: annotation2098160
version: 1
top level: PmeI site
location: https://synbiohub.org/public/igem/BBa_K316013/annotation2098160/range2098160/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K316013/annotation2098160/range2098160/1
type: Range
persistent identity: range2098160
display id: range2098160
version: 1
top level: PmeI site
direction: direction/forward
start: 1
end: 4
orientation: inline

3' product
type: SequenceAnnotation
persistent identity: annotation2098166
display id: annotation2098166
version: 1
top level: PmeI site
location: https://synbiohub.org/public/igem/BBa_K316013/annotation2098166/range2098166/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K316013/annotation2098166/range2098166/1
type: Range
persistent identity: range2098166
display id: range2098166
version: 1
top level: PmeI site
direction: direction/forward
start: 5
end: 8
orientation: inline

https://synbiohub.org/public/igem/BBa_K316027_sequence/1
type: Sequence
persistent identity: BBa K316027 sequence
display id: BBa_K316027_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtttgcaaaacgattcaaaacctctttactgccgttattcgctggatttttattgctgtttcatttggttctggcaggaccggcggctgcgagtgctgaaacggcgaacaaatcgaatgagcttacagcaccgtcgatcaaaagcggaaccattcttcatgcatggaattggtcgttcaatacgttaaaacacaatatgaaggatattcatgatgcaggatatacagccattcagacatctccgattaaccaagtaaaggaagggaatcaaggagataaaagcatgtcgaactggtactggctgtatcagccgacatcgtatcaaattggcaaccgttacttaggtactgaacaagaatttaaagaaatgtgtgcagccgctgaagaatatggcataaaggtcattgttgacgcggtcatcaatcataccaccagtgattatgccgcgatttccaatgaggttaagagtattccaaactggacacatggaaacacacaaattaaaaactggtctgatcgatactagagaattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgttactagagaaaggtggtgaatactagatgaggaggatatatttgaatacatacgaacaaattaataaagtgaaaaaaatacttcggaaacatttaaaaaataaccttattggtacttacatgtttggatcaggagttgagagtggactaaaaccaaatagtgatcttgactttttagtcgtcgtatctgaaccattgacagatcaaagtaaagaaatacttatacaaaaaattagacctatttcaaaaaaaataggagataaaagcaacttacgatatattgaattaacaattattattcagcaagaaatggtaccgtggaatcatcctcccaaacaagaatttatttatggagaatggttacaagagctttatgaacaaggatacattcctcagaaggaattaaattcagatttaaccataatgctttaccaagcaaaacgaaaaaataaaagaatatacggaaattatgacttagaggaattactacctgatattccattttctgatgtgagaagagccattatggattcgtcagaggaattaatagataattatcaggatgatgaaaccaactctatattaactttatgccgtatgattttaactatggacacgggtaaaatcataccaaaagatattgcgggaaatgcagtggctgaatcttctccattagaacatagggagagaattttgttagcagttcgtagttatcttggagagaatattgaatggactaatgaaaatgtaaatttaactataaactatttaaataacagattaaaaaaattataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgttactagagaaaggtggtgaatactagatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcgg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143001_sequence/1
type: Sequence
persistent identity: BBa K143001 sequence
display id: BBa_K143001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtttgcaaaacgattcaaaacctctttactgccgttattcgctggatttttattgctgtttcatttggttctggcaggaccggcggctgcgagtgctgaaacggcgaacaaatcgaatgagcttacagcaccgtcgatcaaaagcggaaccattcttcatgcatggaattggtcgttcaatacgttaaaacacaatatgaaggatattcatgatgcaggatatacagccattcagacatctccgattaaccaagtaaaggaagggaatcaaggagataaaagcatgtcgaactggtactggctgtatcagccgacatcgtatcaaattggcaaccgttacttaggtactgaacaagaatttaaagaaatgtgtgcagccgctgaagaatatggcataaaggtcattgttgacgcggtcatcaatcataccaccagtgattatgccgcgatttccaatgaggttaagagtattccaaactggacacatggaaacacacaaattaaaaactggtctgatcga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143002_sequence/1
type: Sequence
persistent identity: BBa K143002 sequence
display id: BBa_K143002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atccgtttaggctgggcggtgatagcttctcgttcaggcagtacgcctcttttcttttccagacctgagggaggcggaaatggtgtgaggttcccggggaaaagccaaataggcgatcgcgggagtgctttatttgaagatcaggctatcactgcggtcaatagatttcacaatgtgatggctggacagcctgaggaactctcgaacccgaatggaaacaaccagatatttatgaatcagcgcggctcacatggcgttgtgctggcaaatgcaggttcatcctctgtctctatcaatacggcaacaaaattgcctgatggcaggtatgacaataaagctggagcgggttcatttcaagtgaacgatggtaaactgacaggcacgatcaatgccaggtctgtagctgtgctttatcctgatgatattgcaaaagcgcctcatgttttccttgagaattacaaaacaggtgtaacacattctttcaatgatcaactgacgattaccttgcgtgcagatgcgaatacaacaaaagccgtttatcaaatcaataatggaccagagacggcgtttaaggatggagatcaattcacaatcggaaaaggagatccatttggcaaaacatacaccatcatgttaaaaggaacgaacagtgatggtgtaacgaggaccgagaaatacagttttgttaaaagagatccagcgtcggccaaaaccatcggctatcaaaatccgaatcattggagccaggtaaatgcttatatctataaacatgatgggagccgagtaattgaattgaccggatcttggcctggaaaaccaatgactaaaaatgcagacggaatttacacgctgacgctgcctgcggacacggatacaaccaacgcaaaagtgatttttaataatggcagcgcccaagtgcccggtcagaatcagcctggctttgattacgtgctaaatggtttatataatgactcgggcttaagcggttctcttccccattga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143062_sequence/1
type: Sequence
persistent identity: BBa K143062 sequence
display id: BBa_K143062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaaacggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgtcaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143053_sequence/1
type: Sequence
persistent identity: BBa K143053 sequence
display id: BBa_K143053_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgttactagagaaaggtggtgaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143065_sequence/1
type: Sequence
persistent identity: BBa K143065 sequence
display id: BBa_K143065_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaggaggatatatttgaatacatacgaacaaattaataaagtgaaaaaaatacttcggaaacatttaaaaaataaccttattggtacttacatgtttggatcaggagttgagagtggactaaaaccaaatagtgatcttgactttttagtcgtcgtatctgaaccattgacagatcaaagtaaagaaatacttatacaaaaaattagacctatttcaaaaaaaataggagataaaagcaacttacgatatattgaattaacaattattattcagcaagaaatggtaccgtggaatcatcctcccaaacaagaatttatttatggagaatggttacaagagctttatgaacaaggatacattcctcagaaggaattaaattcagatttaaccataatgctttaccaagcaaaacgaaaaaataaaagaatatacggaaattatgacttagaggaattactacctgatattccattttctgatgtgagaagagccattatggattcgtcagaggaattaatagataattatcaggatgatgaaaccaactctatattaactttatgccgtatgattttaactatggacacgggtaaaatcataccaaaagatattgcgggaaatgcagtggctgaatcttctccattagaacatagggagagaattttgttagcagttcgtagttatcttggagagaatattgaatggactaatgaaaatgtaaatttaactataaactatttaaataacagattaaaaaaattataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K316014_sequence/1
type: Sequence
persistent identity: BBa K316014 sequence
display id: BBa_K316014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atctcctagaatatatattatgtaaacttactagaggaaatttc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143012_sequence/1
type: Sequence
persistent identity: BBa K143012 sequence
display id: BBa_K143012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143031_sequence/1
type: Sequence
persistent identity: BBa K143031 sequence
display id: BBa_K143031_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaggaggatatatttgaatacatacgaacaaattaataaagtgaaaaaaatacttcggaaacatttaaaaaataaccttattggtacttacatgtttggatcaggagttgagagtggactaaaaccaaatagtgatcttgactttttagtcgtcgtatctgaaccattgacagatcaaagtaaagaaatacttatacaaaaaattagacctatttcaaaaaaaataggagataaaagcaacttacgatatattgaattaacaattattattcagcaagaaatggtaccgtggaatcatcctcccaaacaagaatttatttatggagaatggttacaagagctttatgaacaaggatacattcctcagaaggaattaaattcagatttaaccataatgctttaccaagcaaaacgaaaaaataaaagaatatacggaaattatgacttagaggaattactacctgatattccattttctgatgtgagaagagccattatggattcgtcagaggaattaatagataattatcaggatgatgaaaccaactctatattaactttatgccgtatgattttaactatggacacgggtaaaatcataccaaaagatattgcgggaaatgcagtggctgaatcttctccattagaacatagggagagaattttgttagcagttcgtagttatcttggagagaatattgaatggactaatgaaaatgtaaatttaactataaactatttaaataacagattaaaaaaattataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143033_sequence/1
type: Sequence
persistent identity: BBa K143033 sequence
display id: BBa_K143033_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaaacggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgtcaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K143021_sequence/1
type: Sequence
persistent identity: BBa K143021 sequence
display id: BBa_K143021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaggtggtgaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K316002_sequence/1
type: Sequence
persistent identity: BBa K316002 sequence
display id: BBa_K316002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atctcctagaatatatattatgtaaact
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K316013_sequence/1
type: Sequence
persistent identity: BBa K316013 sequence
display id: BBa_K316013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaaatttc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z