LOCUS       BBa_K316032             1348 bp    DNA     linear       20-Dec-2024
DEFINITION  Pveg-spoVG-LytC-Glycine Linker-TEV Cleavage Site-AIP-His Tag
ACCESSION   BBa_K316032
VERSION     BBa_K316032.1  
FEATURES             Location/Qualifiers
     promoter        63..68
                     /label=Sigma A-35
     promoter        86..91
                     /label=Sigma A-35
     promoter        1..97
                     /label=BBa_K316001
     RBS             106..120
                     /label=sRBS
     misc_feature    127..129
                     /label=Start
     CDS             127..1081
                     /label=LytC Cell Wall Binding Domain
     CDS             1082..1116
                     /label=Glycine Linker
     CDS             1117..1170
                     /label=TEV Cleavage Site
     CDS             1171..1221
                     /label=Auto Inducing Peptide
     misc_feature    1222..1239
                     /label=His Tag
     misc_feature    1240..1242
                     /label=Stop Codon
     misc_feature    1243..1245
                     /label=Stop Codon
     misc_feature    106..1245
                     /label=BBa_K316042
     stem_loop       1261..1280
                     /label=T7 TE
     misc_feature    1287..1287
                     /label=stop
     polyA_site      1281..1294
                     /label=polya
     misc_feature    1254..1294
                     /label=BBa_B0012
     terminator      1254..1294
                     /label=BBa_B0012
     stem_loop       1315..1337
                     /label=stem_loop
     misc_feature    1303..1348
                     /label=BBa_B0011
     terminator      1303..1348
                     /label=BBa_B0011
     terminator      1254..1348
                     /label=BBa_B0014
ORIGIN
        1 aattttgtca aaataatttt attgacaacg tcttattaac gttgatataa tttaaatttt
       61 atttgacaaa aatgggctcg tgttgtacaa taaatgttac tagagtcttc agaaaggagt
      121 tactagatgc gttcttatat aaaagtccta acaatgtgtt ttctggggct catacttttt
      181 gtgccaacag ctttggccga taactcagtg aaaagagttg ggggaagcaa tagatacggc
      241 actgctgtac aaatatcaaa gcaaatgtat tcaacagcaa gtacagctgt aattgttggt
      301 gggagttcct atgcagatgc tatttcagca gcacctcttg cttaccagaa gaatgcgcca
      361 ttgctttaca ctaattctga taagctttca tatgaaacga aaacaagatt gaaagagatg
      421 cagactaaaa atgtaattat tgtaggcgga acacctgctg tttcttctaa cactgctaac
      481 cagattaaaa gcttggggat aagtattaaa cgaattgcag gaagcaaccg ttatgatacg
      541 gctgcacggg tggcaaaagc gatgggtgcg acttcaaaag ctgttatttt gaacggcttc
      601 ttatatgcag acgctccggc cgtcatccct tatgcagcga aaaacgggta tccaattctt
      661 tttacaaata aaacatctat aaatagtgcg actacgtctg tgataaaaga taagggaatt
      721 tcgagtaccg ttgttgtagg aggcactgga agtatcagca atacggtata caacaagtta
      781 ccttctccta caagaattag cggttcaaac agatatgagc ttgctgcaaa tatcgtacaa
      841 aaacttaatt tatcaacaag caccgtatat gtaagcaatg gattcagcta ccctgactct
      901 attgcaggag ctacactggc agctaagaag aagcaatctc ttattcttac aaatggtgaa
      961 aatttatcta caggagcccg taaaattatt ggaagtaaaa acatgtcaaa ctttatgatt
     1021 atcggaaaca ctcctgccgt aagcacaaag gttgctaatc agctaaagaa tccagttgta
     1081 agccgcggat caagagcagg tggaggcgga ggcggcggag gtggaggtga aaatctttat
     1141 tttcaaggag gcaaactggg aggcggcgga gaaatgcgtc tgtcaaaatt ctttcgcgat
     1201 tttattcttc aaagaaaaaa gcatcaccat catcaccatt aataatacta gagtcacact
     1261 ggctcacctt cgggtgggcc tttctgcgtt tatatactag agagagaata taaaaagcca
     1321 gattattaat ccggcttttt tattattt
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