BBa_K316001 1 Pveg pVeg Constitutive promoter for Veg locus from B. subtilis 2010-10-11T11:00:00Z 2015-05-08T01:11:56Z PCR from existing biobrick K143053 using Pfu polymerase II Released HQ 2013 This part is identical to the sequence submitted by Imperial 2008 team, this part was produced from K143053 by PCR. PVeg is a constitutive promoter controlled by Sigma factor A. This promoter has two binding sites which leads to high expression of downstream genes. There is some evidence that the sporulation master regulator the spoOA can interact with pVeg although the mechanism is not known. false false _440_ 0 7480 9 In stock false PCR using Pfu polymerase to avoid mutations false IC 2010 Team annotation2085549 1 Sigma A-35 range2085549 1 63 68 annotation2085550 1 Sigma A-35 range2085550 1 86 91 BBa_B0014 1 BBa_B0014 double terminator (B0012-B0011) 2003-07-15T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0012 and BBa_B0011 false true _1_ 0 24 7 In stock false true Reshma Shetty component939303 1 BBa_B0012 component939311 1 BBa_B0011 annotation939311 1 BBa_B0011 range939311 1 50 95 annotation939303 1 BBa_B0012 range939303 1 1 41 BBa_K316032 1 P-RBS-CWB- Pveg-spoVG-LytC-Glycine Linker-TEV Cleavage Site-AIP-His Tag 2010-10-19T11:00:00Z 2015-05-08T01:11:57Z Genome PCR from B. subtilis genome (strain) with linker-TEV cleavable site-ComC synthesised and attached via a PME site within LytC gene We designed a protein that carries our signal peptide ComC out of the cell where a protease has access to it. The protease can then proceed to cleave ComC off the protein, allowing quorum sensing via the the ComCDE system to take place. One big problem we have to overcome is the cell wall that will obstruct the protease???s access to the protein carrying the signal peptide. The Detection module consists of a cell wall anchor, a signal peptide called ComC, and a linker that connects the two and is specifically designed to be cleaved by the protease we want to detect false false _440_ 0 7480 9 Not in stock false Pfu DNA polymerase was used to minimise PCR errors false IC 2010 Team component2111937 1 BBa_B0014 component2111930 1 BBa_K316042 component2111920 1 BBa_K316001 annotation2111920 1 BBa_K316001 range2111920 1 1 97 annotation2111937 1 BBa_B0014 range2111937 1 1254 1348 annotation2111930 1 BBa_K316042 range2111930 1 106 1245 BBa_K316042 1 LytC-Gly-T LytC - Glycine Linker - TEV Cleavage Site - AIP - His Tag 2010-10-26T11:00:00Z 2015-05-08T01:11:57Z LytC cell wall binding domain <bbpart>BBa_K316030</bbpart> was extracted from ??????Bacillus subtilis?????? genome using PCR with Pfu DNA polymerase. The linker, TEV protease cleavage site and his-tagged auto inducing peptide were produced by direct synthesis by mwg Eurofins[http://www.eurofinsdna.com/home.html]. The two parts were combined using AccI[http://www.neb.com/nebecomm/products/productR0161.asp] restriction endonuclease. '''Introduction:''' false false _440_ 0 7480 9 Not in stock false Design false IC 2010 Team annotation2109637 1 His Tag range2109637 1 1117 1134 annotation2109635 1 TEV Cleavage Site range2109635 1 1012 1065 annotation2109627 1 Glycine Linker range2109627 1 977 1011 annotation2107923 1 sRBS range2107923 1 1 15 annotation2109636 1 Auto Inducing Peptide range2109636 1 1066 1116 annotation2107956 1 Start range2107956 1 22 24 annotation2107962 1 LytC Cell Wall Binding Domain range2107962 1 22 976 annotation2109638 1 Stop Codon range2109638 1 1135 1137 annotation2109639 1 Stop Codon range2109639 1 1138 1140 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_B0011 1 BBa_B0011 LuxICDABEG (+/-) 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>. Released HQ 2013 Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p> false false _1_ 0 24 7 In stock false <P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A-&gt;G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P> true Reshma Shetty annotation7019 1 BBa_B0011 range7019 1 1 46 annotation1683 1 stem_loop range1683 1 13 35 BBa_B0014_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_K316001_sequence 1 aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgt BBa_K316042_sequence 1 tcttcagaaaggagttactagatgcgttcttatataaaagtcctaacaatgtgttttctggggctcatactttttgtgccaacagctttggccgataactcagtgaaaagagttgggggaagcaatagatacggcactgctgtacaaatatcaaagcaaatgtattcaacagcaagtacagctgtaattgttggtgggagttcctatgcagatgctatttcagcagcacctcttgcttaccagaagaatgcgccattgctttacactaattctgataagctttcatatgaaacgaaaacaagattgaaagagatgcagactaaaaatgtaattattgtaggcggaacacctgctgtttcttctaacactgctaaccagattaaaagcttggggataagtattaaacgaattgcaggaagcaaccgttatgatacggctgcacgggtggcaaaagcgatgggtgcgacttcaaaagctgttattttgaacggcttcttatatgcagacgctccggccgtcatcccttatgcagcgaaaaacgggtatccaattctttttacaaataaaacatctataaatagtgcgactacgtctgtgataaaagataagggaatttcgagtaccgttgttgtaggaggcactggaagtatcagcaatacggtatacaacaagttaccttctcctacaagaattagcggttcaaacagatatgagcttgctgcaaatatcgtacaaaaacttaatttatcaacaagcaccgtatatgtaagcaatggattcagctaccctgactctattgcaggagctacactggcagctaagaagaagcaatctcttattcttacaaatggtgaaaatttatctacaggagcccgtaaaattattggaagtaaaaacatgtcaaactttatgattatcggaaacactcctgccgtaagcacaaaggttgctaatcagctaaagaatccagttgtaagccgcggatcaagagcaggtggaggcggaggcggcggaggtggaggtgaaaatctttattttcaaggaggcaaactgggaggcggcggagaaatgcgtctgtcaaaattctttcgcgattttattcttcaaagaaaaaagcatcaccatcatcaccattaataa BBa_K316032_sequence 1 aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgttactagagtcttcagaaaggagttactagatgcgttcttatataaaagtcctaacaatgtgttttctggggctcatactttttgtgccaacagctttggccgataactcagtgaaaagagttgggggaagcaatagatacggcactgctgtacaaatatcaaagcaaatgtattcaacagcaagtacagctgtaattgttggtgggagttcctatgcagatgctatttcagcagcacctcttgcttaccagaagaatgcgccattgctttacactaattctgataagctttcatatgaaacgaaaacaagattgaaagagatgcagactaaaaatgtaattattgtaggcggaacacctgctgtttcttctaacactgctaaccagattaaaagcttggggataagtattaaacgaattgcaggaagcaaccgttatgatacggctgcacgggtggcaaaagcgatgggtgcgacttcaaaagctgttattttgaacggcttcttatatgcagacgctccggccgtcatcccttatgcagcgaaaaacgggtatccaattctttttacaaataaaacatctataaatagtgcgactacgtctgtgataaaagataagggaatttcgagtaccgttgttgtaggaggcactggaagtatcagcaatacggtatacaacaagttaccttctcctacaagaattagcggttcaaacagatatgagcttgctgcaaatatcgtacaaaaacttaatttatcaacaagcaccgtatatgtaagcaatggattcagctaccctgactctattgcaggagctacactggcagctaagaagaagcaatctcttattcttacaaatggtgaaaatttatctacaggagcccgtaaaattattggaagtaaaaacatgtcaaactttatgattatcggaaacactcctgccgtaagcacaaaggttgctaatcagctaaagaatccagttgtaagccgcggatcaagagcaggtggaggcggaggcggcggaggtggaggtgaaaatctttattttcaaggaggcaaactgggaggcggcggagaaatgcgtctgtcaaaattctttcgcgattttattcttcaaagaaaaaagcatcaccatcatcaccattaataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_B0011_sequence 1 agagaatataaaaagccagattattaatccggcttttttattattt BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z