LOCUS       BBa_K316033             1387 bp    DNA     linear       01-Jun-2024
DEFINITION  Pveg-spoVG-LytC-Flexible Linker-TEV Cleavage Site-AIP-His Tag
ACCESSION   BBa_K316033
VERSION     BBa_K316033.1  
FEATURES             Location/Qualifiers
     promoter        63..68
                     /label=Sigma A-35
     promoter        86..91
                     /label=Sigma A-35
     promoter        1..97
                     /label=BBa_K316001
     RBS             106..120
                     /label=sRBS
     CDS             127..1081
                     /label=LytC Cell Wall Binding Domain
     misc_feature    127..129
                     /label=start
     CDS             1082..1155
                     /label=Flexible Linker
     CDS             1156..1209
                     /label=TEV Cleavage Site
     CDS             1210..1260
                     /label=Auto Inducing Peptide
     misc_feature    1261..1278
                     /label=His Tag
     misc_feature    1279..1281
                     /label=Stop Codon
     misc_feature    1282..1284
                     /label=Stop Codon
     misc_feature    106..1284
                     /label=BBa_K316043
     stem_loop       1300..1319
                     /label=T7 TE
     misc_feature    1326..1326
                     /label=stop
     polyA_site      1320..1333
                     /label=polya
     misc_feature    1293..1333
                     /label=BBa_B0012
     terminator      1293..1333
                     /label=BBa_B0012
     stem_loop       1354..1376
                     /label=stem_loop
     misc_feature    1342..1387
                     /label=BBa_B0011
     terminator      1342..1387
                     /label=BBa_B0011
     terminator      1293..1387
                     /label=BBa_B0014
ORIGIN
        1 aattttgtca aaataatttt attgacaacg tcttattaac gttgatataa tttaaatttt
       61 atttgacaaa aatgggctcg tgttgtacaa taaatgttac tagagtcttc agaaaggagt
      121 tactagatgc gttcttatat aaaagtccta acaatgtgtt ttctggggct catacttttt
      181 gtgccaacag ctttggccga taactcagtg aaaagagttg ggggaagcaa tagatacggc
      241 actgctgtac aaatatcaaa gcaaatgtat tcaacagcaa gtacagctgt aattgttggt
      301 gggagttcct atgcagatgc tatttcagca gcacctcttg cttaccagaa gaatgcgcca
      361 ttgctttaca ctaattctga taagctttca tatgaaacga aaacaagatt gaaagagatg
      421 cagactaaaa atgtaattat tgtaggcgga acacctgctg tttcttctaa cactgctaac
      481 cagattaaaa gcttggggat aagtattaaa cgaattgcag gaagcaaccg ttatgatacg
      541 gctgcacggg tggcaaaagc gatgggtgcg acttcaaaag ctgttatttt gaacggcttc
      601 ttatatgcag acgctccggc cgtcatccct tatgcagcga aaaacgggta tccaattctt
      661 tttacaaata aaacatctat aaatagtgcg actacgtctg tgataaaaga taagggaatt
      721 tcgagtaccg ttgttgtagg aggcactgga agtatcagca atacggtata caacaagtta
      781 ccttctccta caagaattag cggttcaaac agatatgagc ttgctgcaaa tatcgtacaa
      841 aaacttaatt tatcaacaag caccgtatat gtaagcaatg gattcagcta ccctgactct
      901 attgcaggag ctacactggc agctaagaag aagcaatctc ttattcttac aaatggtgaa
      961 aatttatcta caggagcccg taaaattatt ggaagtaaaa acatgtcaaa ctttatgatt
     1021 atcggaaaca ctcctgccgt aagcacaaag gttgctaatc agctaaagaa tccagttgta
     1081 agtagaggat cccgcgctct gggaggcggc ggctcaggtg gtggcggaag cggcggaggc
     1141 ggttctgccg cagcgggagg tggaggtgaa aatctttatt ttcaaggagg caaactggga
     1201 ggcggcggag aaatgcgtct gtcaaaattc tttcgcgatt ttattcttca aagaaaaaag
     1261 catcaccatc atcaccatta ataatactag agtcacactg gctcaccttc gggtgggcct
     1321 ttctgcgttt atatactaga gagagaatat aaaaagccag attattaatc cggctttttt
     1381 attattt
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