LOCUS       BBa_K316034             1390 bp    DNA     linear       05-Apr-2025
DEFINITION  Pveg-spoVG-LytC-Helical Linker-TEV Cleavage Site-AIP-His Tag
ACCESSION   BBa_K316034
VERSION     BBa_K316034.1  
FEATURES             Location/Qualifiers
     promoter        63..68
                     /label=Sigma A-35
     promoter        86..91
                     /label=Sigma A-35
     promoter        1..97
                     /label=BBa_K316001
     RBS             106..120
                     /label=sRBS
     misc_feature    127..129
                     /label=start
     CDS             127..1081
                     /label=LytC Cell Wall Binding Domain
     CDS             1082..1158
                     /label=Helical Linker
     CDS             1159..1212
                     /label=TEV Cleavage Site
     CDS             1213..1263
                     /label=Auto Inducing Peptide
     misc_feature    1264..1281
                     /label=His Tag
     misc_feature    1282..1284
                     /label=Stop Codon
     misc_feature    1285..1287
                     /label=Stop Codon
     misc_feature    106..1287
                     /label=BBa_K316044
     stem_loop       1303..1322
                     /label=T7 TE
     misc_feature    1329..1329
                     /label=stop
     polyA_site      1323..1336
                     /label=polya
     misc_feature    1296..1336
                     /label=BBa_B0012
     terminator      1296..1336
                     /label=BBa_B0012
     stem_loop       1357..1379
                     /label=stem_loop
     misc_feature    1345..1390
                     /label=BBa_B0011
     terminator      1345..1390
                     /label=BBa_B0011
     terminator      1296..1390
                     /label=BBa_B0014
ORIGIN
        1 aattttgtca aaataatttt attgacaacg tcttattaac gttgatataa tttaaatttt
       61 atttgacaaa aatgggctcg tgttgtacaa taaatgttac tagagtcttc agaaaggagt
      121 tactagatgc gttcttatat aaaagtccta acaatgtgtt ttctggggct catacttttt
      181 gtgccaacag ctttggccga taactcagtg aaaagagttg ggggaagcaa tagatacggc
      241 actgctgtac aaatatcaaa gcaaatgtat tcaacagcaa gtacagctgt aattgttggt
      301 gggagttcct atgcagatgc tatttcagca gcacctcttg cttaccagaa gaatgcgcca
      361 ttgctttaca ctaattctga taagctttca tatgaaacga aaacaagatt gaaagagatg
      421 cagactaaaa atgtaattat tgtaggcgga acacctgctg tttcttctaa cactgctaac
      481 cagattaaaa gcttggggat aagtattaaa cgaattgcag gaagcaaccg ttatgatacg
      541 gctgcacggg tggcaaaagc gatgggtgcg acttcaaaag ctgttatttt gaacggcttc
      601 ttatatgcag acgctccggc cgtcatccct tatgcagcga aaaacgggta tccaattctt
      661 tttacaaata aaacatctat aaatagtgcg actacgtctg tgataaaaga taagggaatt
      721 tcgagtaccg ttgttgtagg aggcactgga agtatcagca atacggtata caacaagtta
      781 ccttctccta caagaattag cggttcaaac agatatgagc ttgctgcaaa tatcgtacaa
      841 aaacttaatt tatcaacaag caccgtatat gtaagcaatg gattcagcta ccctgactct
      901 attgcaggag ctacactggc agctaagaag aagcaatctc ttattcttac aaatggtgaa
      961 aatttatcta caggagcccg taaaattatt ggaagtaaaa acatgtcaaa ctttatgatt
     1021 atcggaaaca ctcctgccgt aagcacaaag gttgctaatc agctaaagaa tccagttgta
     1081 agcagaggct cacgcgcact ggcagaagcc gcagctaagg aagctgctgc aaaagaggcg
     1141 gcggcgaaag ccgcggccgg aggtggaggt gaaaatcttt attttcaagg aggcaaactg
     1201 ggaggcggcg gagaaatgcg tctgtcaaaa ttctttcgcg attttattct tcaaagaaaa
     1261 aagcatcacc atcatcacca ttaataatac tagagtcaca ctggctcacc ttcgggtggg
     1321 cctttctgcg tttatatact agagagagaa tataaaaagc cagattatta atccggcttt
     1381 tttattattt
//