BBa_K316035
1
P-RBS-CWB-
Pveg-spoVG-LytC-Glycine Linker-Elastase Cleavage Site-AIP-His Tag
2010-10-25T11:00:00Z
2015-05-08T01:11:57Z
source
long
false
false
_440_
0
7480
9
Not in stock
false
Please see main page
false
IC 2010 Team
component2111977
1
BBa_B0014
component2111970
1
BBa_K316045
component2111960
1
BBa_K316001
annotation2111977
1
BBa_B0014
range2111977
1
1212
1306
annotation2111970
1
BBa_K316045
range2111970
1
106
1203
annotation2111960
1
BBa_K316001
range2111960
1
1
97
BBa_B0014
1
BBa_B0014
double terminator (B0012-B0011)
2003-07-15T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0012 and BBa_B0011
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component939303
1
BBa_B0012
component939311
1
BBa_B0011
annotation939311
1
BBa_B0011
range939311
1
50
95
annotation939303
1
BBa_B0012
range939303
1
1
41
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1690
1
polya
range1690
1
28
41
annotation1687
1
stop
range1687
1
34
34
annotation1686
1
T7 TE
range1686
1
8
27
BBa_B0011
1
BBa_B0011
LuxICDABEG (+/-)
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
Released HQ 2013
Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p>
false
false
_1_
0
24
7
In stock
false
<P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P>
true
Reshma Shetty
annotation1683
1
stem_loop
range1683
1
13
35
annotation7019
1
BBa_B0011
range7019
1
1
46
BBa_K316001
1
Pveg
pVeg Constitutive promoter for Veg locus from B. subtilis
2010-10-11T11:00:00Z
2015-05-08T01:11:56Z
PCR from existing biobrick K143053 using Pfu polymerase II
Released HQ 2013
This part is identical to the sequence submitted by Imperial 2008 team, this part was produced from K143053 by PCR.
PVeg is a constitutive promoter controlled by Sigma factor A. This promoter has two binding sites which leads to high expression of downstream genes.
There is some evidence that the sporulation master regulator the spoOA can interact with pVeg although the mechanism is not known.
false
false
_440_
0
7480
9
In stock
false
PCR using Pfu polymerase to avoid mutations
false
IC 2010 Team
annotation2085549
1
Sigma A-35
range2085549
1
63
68
annotation2085550
1
Sigma A-35
range2085550
1
86
91
BBa_K316045
1
LytC-Gly-E
LytC - Glycine Linker - Elastase Cleavage Site - AIP - His Tag
2010-10-26T11:00:00Z
2015-05-08T01:11:57Z
source
intro
false
false
_440_
0
7480
9
Not in stock
false
design
false
IC 2010 Team
annotation2109930
1
Stop Codon
range2109930
1
1093
1095
annotation2109927
1
Elastase Cleavage Site
range2109927
1
1012
1023
annotation2107960
1
start
range2107960
1
22
24
annotation2109931
1
Stop Codon
range2109931
1
1096
1098
annotation2109929
1
His Tag
range2109929
1
1075
1092
annotation2107965
1
LytC Cell Wall Binding Domain
range2107965
1
22
976
annotation2107927
1
sRBS
range2107927
1
1
15
annotation2109928
1
Auto Inducing Peptide
range2109928
1
1024
1074
annotation2109926
1
Glycine Linker
range2109926
1
977
1011
BBa_K316035_sequence
1
aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgttactagagtcttcagaaaggagttactagatgcgttcttatataaaagtcctaacaatgtgttttctggggctcatactttttgtgccaacagctttggccgataactcagtgaaaagagttgggggaagcaatagatacggcactgctgtacaaatatcaaagcaaatgtattcaacagcaagtacagctgtaattgttggtgggagttcctatgcagatgctatttcagcagcacctcttgcttaccagaagaatgcgccattgctttacactaattctgataagctttcatatgaaacgaaaacaagattgaaagagatgcagactaaaaatgtaattattgtaggcggaacacctgctgtttcttctaacactgctaaccagattaaaagcttggggataagtattaaacgaattgcaggaagcaaccgttatgatacggctgcacgggtggcaaaagcgatgggtgcgacttcaaaagctgttattttgaacggcttcttatatgcagacgctccggccgtcatcccttatgcagcgaaaaacgggtatccaattctttttacaaataaaacatctataaatagtgcgactacgtctgtgataaaagataagggaatttcgagtaccgttgttgtaggaggcactggaagtatcagcaatacggtatacaacaagttaccttctcctacaagaattagcggttcaaacagatatgagcttgctgcaaatatcgtacaaaaacttaatttatcaacaagcaccgtatatgtaagcaatggattcagctaccctgactctattgcaggagctacactggcagctaagaagaagcaatctcttattcttacaaatggtgaaaatttatctacaggagcccgtaaaattattggaagtaaaaacatgtcaaactttatgattatcggaaacactcctgccgtaagcacaaaggttgctaatcagctaaagaatccagttgtaagccgcggatcaagagcaggtggaggcggaggcggctcatggccgctggaaatgcgtctgtcaaaattctttcgcgattttattcttcaaagaaaaaagcatcaccatcatcaccattaataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0014_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_K316001_sequence
1
aattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgt
BBa_K316045_sequence
1
tcttcagaaaggagttactagatgcgttcttatataaaagtcctaacaatgtgttttctggggctcatactttttgtgccaacagctttggccgataactcagtgaaaagagttgggggaagcaatagatacggcactgctgtacaaatatcaaagcaaatgtattcaacagcaagtacagctgtaattgttggtgggagttcctatgcagatgctatttcagcagcacctcttgcttaccagaagaatgcgccattgctttacactaattctgataagctttcatatgaaacgaaaacaagattgaaagagatgcagactaaaaatgtaattattgtaggcggaacacctgctgtttcttctaacactgctaaccagattaaaagcttggggataagtattaaacgaattgcaggaagcaaccgttatgatacggctgcacgggtggcaaaagcgatgggtgcgacttcaaaagctgttattttgaacggcttcttatatgcagacgctccggccgtcatcccttatgcagcgaaaaacgggtatccaattctttttacaaataaaacatctataaatagtgcgactacgtctgtgataaaagataagggaatttcgagtaccgttgttgtaggaggcactggaagtatcagcaatacggtatacaacaagttaccttctcctacaagaattagcggttcaaacagatatgagcttgctgcaaatatcgtacaaaaacttaatttatcaacaagcaccgtatatgtaagcaatggattcagctaccctgactctattgcaggagctacactggcagctaagaagaagcaatctcttattcttacaaatggtgaaaatttatctacaggagcccgtaaaattattggaagtaaaaacatgtcaaactttatgattatcggaaacactcctgccgtaagcacaaaggttgctaatcagctaaagaatccagttgtaagccgcggatcaagagcaggtggaggcggaggcggctcatggccgctggaaatgcgtctgtcaaaattctttcgcgattttattcttcaaagaaaaaagcatcaccatcatcaccattaataa
BBa_B0011_sequence
1
agagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z