LOCUS BBa_K316042 1140 bp DNA linear 25-Nov-2024 DEFINITION LytC - Glycine Linker - TEV Cleavage Site - AIP - His Tag ACCESSION BBa_K316042 VERSION BBa_K316042.1 FEATURES Location/Qualifiers RBS 1..15 /label=sRBS misc_feature 22..24 /label=Start CDS 22..976 /label=LytC Cell Wall Binding Domain CDS 977..1011 /label=Glycine Linker CDS 1012..1065 /label=TEV Cleavage Site CDS 1066..1116 /label=Auto Inducing Peptide misc_feature 1117..1134 /label=His Tag misc_feature 1135..1137 /label=Stop Codon misc_feature 1138..1140 /label=Stop Codon ORIGIN 1 tcttcagaaa ggagttacta gatgcgttct tatataaaag tcctaacaat gtgttttctg 61 gggctcatac tttttgtgcc aacagctttg gccgataact cagtgaaaag agttggggga 121 agcaatagat acggcactgc tgtacaaata tcaaagcaaa tgtattcaac agcaagtaca 181 gctgtaattg ttggtgggag ttcctatgca gatgctattt cagcagcacc tcttgcttac 241 cagaagaatg cgccattgct ttacactaat tctgataagc tttcatatga aacgaaaaca 301 agattgaaag agatgcagac taaaaatgta attattgtag gcggaacacc tgctgtttct 361 tctaacactg ctaaccagat taaaagcttg gggataagta ttaaacgaat tgcaggaagc 421 aaccgttatg atacggctgc acgggtggca aaagcgatgg gtgcgacttc aaaagctgtt 481 attttgaacg gcttcttata tgcagacgct ccggccgtca tcccttatgc agcgaaaaac 541 gggtatccaa ttctttttac aaataaaaca tctataaata gtgcgactac gtctgtgata 601 aaagataagg gaatttcgag taccgttgtt gtaggaggca ctggaagtat cagcaatacg 661 gtatacaaca agttaccttc tcctacaaga attagcggtt caaacagata tgagcttgct 721 gcaaatatcg tacaaaaact taatttatca acaagcaccg tatatgtaag caatggattc 781 agctaccctg actctattgc aggagctaca ctggcagcta agaagaagca atctcttatt 841 cttacaaatg gtgaaaattt atctacagga gcccgtaaaa ttattggaag taaaaacatg 901 tcaaacttta tgattatcgg aaacactcct gccgtaagca caaaggttgc taatcagcta 961 aagaatccag ttgtaagccg cggatcaaga gcaggtggag gcggaggcgg cggaggtgga 1021 ggtgaaaatc tttattttca aggaggcaaa ctgggaggcg gcggagaaat gcgtctgtca 1081 aaattctttc gcgattttat tcttcaaaga aaaaagcatc accatcatca ccattaataa //