BBa_K316042 1 LytC-Gly-T LytC - Glycine Linker - TEV Cleavage Site - AIP - His Tag 2010-10-26T11:00:00Z 2015-05-08T01:11:57Z LytC cell wall binding domain <bbpart>BBa_K316030</bbpart> was extracted from ??????Bacillus subtilis?????? genome using PCR with Pfu DNA polymerase. The linker, TEV protease cleavage site and his-tagged auto inducing peptide were produced by direct synthesis by mwg Eurofins[http://www.eurofinsdna.com/home.html]. The two parts were combined using AccI[http://www.neb.com/nebecomm/products/productR0161.asp] restriction endonuclease. '''Introduction:''' false false _440_ 0 7480 9 Not in stock false Design false IC 2010 Team annotation2109637 1 His Tag range2109637 1 1117 1134 annotation2109627 1 Glycine Linker range2109627 1 977 1011 annotation2109639 1 Stop Codon range2109639 1 1138 1140 annotation2109636 1 Auto Inducing Peptide range2109636 1 1066 1116 annotation2107923 1 sRBS range2107923 1 1 15 annotation2107956 1 Start range2107956 1 22 24 annotation2107962 1 LytC Cell Wall Binding Domain range2107962 1 22 976 annotation2109638 1 Stop Codon range2109638 1 1135 1137 annotation2109635 1 TEV Cleavage Site range2109635 1 1012 1065 BBa_K316042_sequence 1 tcttcagaaaggagttactagatgcgttcttatataaaagtcctaacaatgtgttttctggggctcatactttttgtgccaacagctttggccgataactcagtgaaaagagttgggggaagcaatagatacggcactgctgtacaaatatcaaagcaaatgtattcaacagcaagtacagctgtaattgttggtgggagttcctatgcagatgctatttcagcagcacctcttgcttaccagaagaatgcgccattgctttacactaattctgataagctttcatatgaaacgaaaacaagattgaaagagatgcagactaaaaatgtaattattgtaggcggaacacctgctgtttcttctaacactgctaaccagattaaaagcttggggataagtattaaacgaattgcaggaagcaaccgttatgatacggctgcacgggtggcaaaagcgatgggtgcgacttcaaaagctgttattttgaacggcttcttatatgcagacgctccggccgtcatcccttatgcagcgaaaaacgggtatccaattctttttacaaataaaacatctataaatagtgcgactacgtctgtgataaaagataagggaatttcgagtaccgttgttgtaggaggcactggaagtatcagcaatacggtatacaacaagttaccttctcctacaagaattagcggttcaaacagatatgagcttgctgcaaatatcgtacaaaaacttaatttatcaacaagcaccgtatatgtaagcaatggattcagctaccctgactctattgcaggagctacactggcagctaagaagaagcaatctcttattcttacaaatggtgaaaatttatctacaggagcccgtaaaattattggaagtaaaaacatgtcaaactttatgattatcggaaacactcctgccgtaagcacaaaggttgctaatcagctaaagaatccagttgtaagccgcggatcaagagcaggtggaggcggaggcggcggaggtggaggtgaaaatctttattttcaaggaggcaaactgggaggcggcggagaaatgcgtctgtcaaaattctttcgcgattttattcttcaaagaaaaaagcatcaccatcatcaccattaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z