LOCUS BBa_K316043 1179 bp DNA linear 22-Nov-2024 DEFINITION LytC - Flexible Linker - TEV Cleavage Site - AIP - His Tag ACCESSION BBa_K316043 VERSION BBa_K316043.1 FEATURES Location/Qualifiers RBS 1..15 /label=sRBS CDS 22..976 /label=LytC Cell Wall Binding Domain misc_feature 22..24 /label=start CDS 977..1050 /label=Flexible Linker CDS 1051..1104 /label=TEV Cleavage Site CDS 1105..1155 /label=Auto Inducing Peptide misc_feature 1156..1173 /label=His Tag misc_feature 1174..1176 /label=Stop Codon misc_feature 1177..1179 /label=Stop Codon ORIGIN 1 tcttcagaaa ggagttacta gatgcgttct tatataaaag tcctaacaat gtgttttctg 61 gggctcatac tttttgtgcc aacagctttg gccgataact cagtgaaaag agttggggga 121 agcaatagat acggcactgc tgtacaaata tcaaagcaaa tgtattcaac agcaagtaca 181 gctgtaattg ttggtgggag ttcctatgca gatgctattt cagcagcacc tcttgcttac 241 cagaagaatg cgccattgct ttacactaat tctgataagc tttcatatga aacgaaaaca 301 agattgaaag agatgcagac taaaaatgta attattgtag gcggaacacc tgctgtttct 361 tctaacactg ctaaccagat taaaagcttg gggataagta ttaaacgaat tgcaggaagc 421 aaccgttatg atacggctgc acgggtggca aaagcgatgg gtgcgacttc aaaagctgtt 481 attttgaacg gcttcttata tgcagacgct ccggccgtca tcccttatgc agcgaaaaac 541 gggtatccaa ttctttttac aaataaaaca tctataaata gtgcgactac gtctgtgata 601 aaagataagg gaatttcgag taccgttgtt gtaggaggca ctggaagtat cagcaatacg 661 gtatacaaca agttaccttc tcctacaaga attagcggtt caaacagata tgagcttgct 721 gcaaatatcg tacaaaaact taatttatca acaagcaccg tatatgtaag caatggattc 781 agctaccctg actctattgc aggagctaca ctggcagcta agaagaagca atctcttatt 841 cttacaaatg gtgaaaattt atctacagga gcccgtaaaa ttattggaag taaaaacatg 901 tcaaacttta tgattatcgg aaacactcct gccgtaagca caaaggttgc taatcagcta 961 aagaatccag ttgtaagtag aggatcccgc gctctgggag gcggcggctc aggtggtggc 1021 ggaagcggcg gaggcggttc tgccgcagcg ggaggtggag gtgaaaatct ttattttcaa 1081 ggaggcaaac tgggaggcgg cggagaaatg cgtctgtcaa aattctttcg cgattttatt 1141 cttcaaagaa aaaagcatca ccatcatcac cattaataa //