LOCUS       BBa_K317023             3275 bp    DNA     linear       22-Nov-2024
DEFINITION  RBS-Antigen 43-Double terminator
ACCESSION   BBa_K317023
VERSION     BBa_K317023.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..3138
                     /label=BBa_K317008
     stem_loop       3158..3201
                     /label=stem_loop
     misc_feature    3147..3226
                     /label=BBa_B0010
     terminator      3147..3226
                     /label=BBa_B0010
     stem_loop       3242..3261
                     /label=T7 TE
     misc_feature    3268..3268
                     /label=stop
     polyA_site      3262..3275
                     /label=polya
     misc_feature    3235..3275
                     /label=BBa_B0012
     terminator      3235..3275
                     /label=BBa_B0012
     terminator      3147..3275
                     /label=BBa_B0015
ORIGIN
        1 aaagaggaga aatactagat gaaacgacat ctgaatacct gctacaggct ggtatggaat
       61 cacatgacgg gcgctttcgt ggttgcctcc gaactggccc gcgcacgggg taaacgtggc
      121 ggtgtggcgg ttgcactgtc tcttgccgca gtcacgtcac tcccggtgct ggctgctgac
      181 atcgttgtgc acccgggaga aaccgtgaac ggcggaacac tggcaaatca tgacaaccag
      241 attgtcttcg gtacgaccaa cggaatgacc atcagtaccg ggctggagta tgggccggat
      301 aacgaggcca ataccggcgg gcaatgggta caggatggcg gaacagccaa caaaacgact
      361 gtcaccagtg gtggtcttca gagagtgaac cccggtggaa gtgtctcaga cacggttatc
      421 agtgccggag gcggacagag ccttcaggga cgggctgtga acaccacgct gaatggtggc
      481 gaacagtgga tgcatgaggg ggcgatagcc acaggaaccg tcattaatga taagggctgg
      541 caggtcgtca agcccggtac agtggcaacg gataccgttg ttaataccgg ggcggaaggg
      601 ggaccggatg cagaaaacgg tgataccggg cagtttgttc gcggggatgc cgtacgcaca
      661 accatcaata aaaacggtcg ccagattgtg agagctgaag gaacggcaaa taccactgtg
      721 gtttatgccg gcggcgacca gactgtacat ggtcacgcac tggataccac gctgaatggg
      781 ggataccagt atgtgcacaa cggcggtaca gcgtctgaca ctgttgtgaa cagtgacggc
      841 tggcagattg tcaaaaacgg gggtgtggcc gggaatacca ccgttaatca gaagggcaga
      901 cttcaggtgg acgccggtgg tacagccacg aatgtcaccc tgaagcaggg cggcgcactg
      961 gttaccagta cggctgcaac cgttaccggc ataaaccgcc tgggagcatt ctctgttgtg
     1021 gagggtaaag ctgataatgt cgtactggaa aatggcggac gcctggatgt gctgaccgga
     1081 cacacagcca ctaatacccg cgtggatgat ggcggaacgc tggatgtccg caacggtggc
     1141 accgccacca ccgtatccat gggaaatggc ggtgtactgc tggccgattc cggtgccgct
     1201 gtcagtggta cccggagcga cggaaaggca ttcagtatcg gaggcggtca ggcggatgcc
     1261 ctgatgctgg aaaaaggcag ttcattcacg ctgaacgccg gtgatacggc cacggatacc
     1321 acggtaaatg gcggactgtt caccgccagg ggcggcacac tggcgggcac caccacgctg
     1381 aataacggcg ccatacttac cctttccggg aagacggtga acaacgatac cctgaccatc
     1441 cgtgaaggcg atccactcct tcagggaggc tctctcaccg gtaacggcag cgtggaaaaa
     1501 tcaggaagtg gcacactcac tgtcagcaac accacactca cccagaaagc cgtcaacctg
     1561 aatgaaggca cgctgacgct gaacgacagt accgtcacca cggatgtcat tgctcagcgc
     1621 ggtacagccc tgaagctgac cggcagcact gtgctgaacg gtgccattga ccccacgaat
     1681 gtcactctcg cctccggtgc cacctggaat atccccgata acgccacggt gcagtcggtg
     1741 gtggatgacc tcagccatgc cggacagatt catttcacct ccacccgcac agggaagttc
     1801 gtaccggcaa ccctgaaagt gaaaaacctg aacggacaga atggcaccat cagcctgcgt
     1861 gtacgcccgg atatggcaca gaacaatgct gacagactgg tcattgacgg cggcagggca
     1921 accggaaaaa ccatcctgaa cctggtgaac gccggcaaca gtgcgtcggg gctggcgacc
     1981 agcggtaagg gtattcaggt ggtggaagcc attaacggtg ccaccacgga ggaaggggcc
     2041 tttgtccagg ggaacaggct tcaggccggt gcctttaact actccctcaa ccgggacagt
     2101 gatgagagct ggtatctgcg cagtgaaaat gcttatcgtg cagaagtccc cctgtatgcc
     2161 tccatgctga cacaggcaat ggactatgac cggattgtgg caggctcccg cagccatcag
     2221 accggtgtaa atggtgaaaa caacagcgtc cgtctcagca ttcagggcgg tcatctcggt
     2281 cacgataaca atggcggtat tgcccgtggg gccacgccgg aaagcagcgg cagctatgga
     2341 ttcgtccgtc tggagggtga cctgatgaga acagaggttg ccggtatgtc tgtgaccgcg
     2401 ggggtatatg gtgctgctgg ccattcttcc gttgatgtta aggatgatga cggctcccgt
     2461 gccggcacgg tccgggatga tgccggcagc ctgggcggat acctgaatct ggtacacacg
     2521 tcctccggcc tgtgggctga cattgtggca cagggaaccc gccacagcat gaaagcgtca
     2581 tcggacaata acgacttccg cgcccggggc tggggctggc tgggctcact ggaaaccggt
     2641 ctgcccttca gtatcactga caacctgatg ctggagccac aacttcagta tacctggcag
     2701 ggactttccc tggatgacgg taaggacaac gccgattatg tgaagttcgg gcatggcagt
     2761 gcacaacatg tgcgtgccgg tttccgtctg ggcagccaca acgatatgac ctttggcgaa
     2821 ggcacctcat cccgtgcccc cctgcgtgac agtgcaaaac acagtgtgag tgaattaccg
     2881 gtgaactggt gggtacagcc ttctgttatc cgcaccttca gcccccgggg agatatgcgt
     2941 gtggggactt ccacagcagg cagcgggatg acgttctctc cctcacagaa tggcacatca
     3001 ctggaccttc aggccggact ggaagcccgt gtccgggaaa atatcaccct gggcgttcag
     3061 gccggttatg cccacagcgt cagcggcagc agcgctgaag ggtataacgg tcaggccaca
     3121 ctgaatgtga ccttctgata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
     3181 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     3241 tggctcacct tcgggtgggc ctttctgcgt ttata
//