LOCUS       BBa_K317025             3318 bp    DNA     linear       23-Dec-2024
DEFINITION  Constitutive promoter(BBa_J23107)-RBS-Antigen 43-Double terminator
ACCESSION   BBa_K317025
VERSION     BBa_K317025.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23107
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..3181
                     /label=BBa_K317008
     stem_loop       3201..3244
                     /label=stem_loop
     misc_feature    3190..3269
                     /label=BBa_B0010
     terminator      3190..3269
                     /label=BBa_B0010
     stem_loop       3285..3304
                     /label=T7 TE
     misc_feature    3311..3311
                     /label=stop
     polyA_site      3305..3318
                     /label=polya
     misc_feature    3278..3318
                     /label=BBa_B0012
     terminator      3278..3318
                     /label=BBa_B0012
     terminator      3190..3318
                     /label=BBa_B0015
ORIGIN
        1 tttacggcta gctcagccct aggtattatg ctagctacta gagaaagagg agaaatacta
       61 gatgaaacga catctgaata cctgctacag gctggtatgg aatcacatga cgggcgcttt
      121 cgtggttgcc tccgaactgg cccgcgcacg gggtaaacgt ggcggtgtgg cggttgcact
      181 gtctcttgcc gcagtcacgt cactcccggt gctggctgct gacatcgttg tgcacccggg
      241 agaaaccgtg aacggcggaa cactggcaaa tcatgacaac cagattgtct tcggtacgac
      301 caacggaatg accatcagta ccgggctgga gtatgggccg gataacgagg ccaataccgg
      361 cgggcaatgg gtacaggatg gcggaacagc caacaaaacg actgtcacca gtggtggtct
      421 tcagagagtg aaccccggtg gaagtgtctc agacacggtt atcagtgccg gaggcggaca
      481 gagccttcag ggacgggctg tgaacaccac gctgaatggt ggcgaacagt ggatgcatga
      541 gggggcgata gccacaggaa ccgtcattaa tgataagggc tggcaggtcg tcaagcccgg
      601 tacagtggca acggataccg ttgttaatac cggggcggaa gggggaccgg atgcagaaaa
      661 cggtgatacc gggcagtttg ttcgcgggga tgccgtacgc acaaccatca ataaaaacgg
      721 tcgccagatt gtgagagctg aaggaacggc aaataccact gtggtttatg ccggcggcga
      781 ccagactgta catggtcacg cactggatac cacgctgaat gggggatacc agtatgtgca
      841 caacggcggt acagcgtctg acactgttgt gaacagtgac ggctggcaga ttgtcaaaaa
      901 cgggggtgtg gccgggaata ccaccgttaa tcagaagggc agacttcagg tggacgccgg
      961 tggtacagcc acgaatgtca ccctgaagca gggcggcgca ctggttacca gtacggctgc
     1021 aaccgttacc ggcataaacc gcctgggagc attctctgtt gtggagggta aagctgataa
     1081 tgtcgtactg gaaaatggcg gacgcctgga tgtgctgacc ggacacacag ccactaatac
     1141 ccgcgtggat gatggcggaa cgctggatgt ccgcaacggt ggcaccgcca ccaccgtatc
     1201 catgggaaat ggcggtgtac tgctggccga ttccggtgcc gctgtcagtg gtacccggag
     1261 cgacggaaag gcattcagta tcggaggcgg tcaggcggat gccctgatgc tggaaaaagg
     1321 cagttcattc acgctgaacg ccggtgatac ggccacggat accacggtaa atggcggact
     1381 gttcaccgcc aggggcggca cactggcggg caccaccacg ctgaataacg gcgccatact
     1441 taccctttcc gggaagacgg tgaacaacga taccctgacc atccgtgaag gcgatccact
     1501 ccttcaggga ggctctctca ccggtaacgg cagcgtggaa aaatcaggaa gtggcacact
     1561 cactgtcagc aacaccacac tcacccagaa agccgtcaac ctgaatgaag gcacgctgac
     1621 gctgaacgac agtaccgtca ccacggatgt cattgctcag cgcggtacag ccctgaagct
     1681 gaccggcagc actgtgctga acggtgccat tgaccccacg aatgtcactc tcgcctccgg
     1741 tgccacctgg aatatccccg ataacgccac ggtgcagtcg gtggtggatg acctcagcca
     1801 tgccggacag attcatttca cctccacccg cacagggaag ttcgtaccgg caaccctgaa
     1861 agtgaaaaac ctgaacggac agaatggcac catcagcctg cgtgtacgcc cggatatggc
     1921 acagaacaat gctgacagac tggtcattga cggcggcagg gcaaccggaa aaaccatcct
     1981 gaacctggtg aacgccggca acagtgcgtc ggggctggcg accagcggta agggtattca
     2041 ggtggtggaa gccattaacg gtgccaccac ggaggaaggg gcctttgtcc aggggaacag
     2101 gcttcaggcc ggtgccttta actactccct caaccgggac agtgatgaga gctggtatct
     2161 gcgcagtgaa aatgcttatc gtgcagaagt ccccctgtat gcctccatgc tgacacaggc
     2221 aatggactat gaccggattg tggcaggctc ccgcagccat cagaccggtg taaatggtga
     2281 aaacaacagc gtccgtctca gcattcaggg cggtcatctc ggtcacgata acaatggcgg
     2341 tattgcccgt ggggccacgc cggaaagcag cggcagctat ggattcgtcc gtctggaggg
     2401 tgacctgatg agaacagagg ttgccggtat gtctgtgacc gcgggggtat atggtgctgc
     2461 tggccattct tccgttgatg ttaaggatga tgacggctcc cgtgccggca cggtccggga
     2521 tgatgccggc agcctgggcg gatacctgaa tctggtacac acgtcctccg gcctgtgggc
     2581 tgacattgtg gcacagggaa cccgccacag catgaaagcg tcatcggaca ataacgactt
     2641 ccgcgcccgg ggctggggct ggctgggctc actggaaacc ggtctgccct tcagtatcac
     2701 tgacaacctg atgctggagc cacaacttca gtatacctgg cagggacttt ccctggatga
     2761 cggtaaggac aacgccgatt atgtgaagtt cgggcatggc agtgcacaac atgtgcgtgc
     2821 cggtttccgt ctgggcagcc acaacgatat gacctttggc gaaggcacct catcccgtgc
     2881 ccccctgcgt gacagtgcaa aacacagtgt gagtgaatta ccggtgaact ggtgggtaca
     2941 gccttctgtt atccgcacct tcagcccccg gggagatatg cgtgtgggga cttccacagc
     3001 aggcagcggg atgacgttct ctccctcaca gaatggcaca tcactggacc ttcaggccgg
     3061 actggaagcc cgtgtccggg aaaatatcac cctgggcgtt caggccggtt atgcccacag
     3121 cgtcagcggc agcagcgctg aagggtataa cggtcaggcc acactgaatg tgaccttctg
     3181 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     3241 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
     3301 ggcctttctg cgtttata
//