LOCUS BBa_K317026 3318 bp DNA linear 22-Dec-2024 DEFINITION Constitutive promoter(BBa_J23109)-RBS-Antigen 43-Double terminator ACCESSION BBa_K317026 VERSION BBa_K317026.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23109 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 62..3181 /label=BBa_K317008 stem_loop 3201..3244 /label=stem_loop misc_feature 3190..3269 /label=BBa_B0010 terminator 3190..3269 /label=BBa_B0010 stem_loop 3285..3304 /label=T7 TE misc_feature 3311..3311 /label=stop polyA_site 3305..3318 /label=polya misc_feature 3278..3318 /label=BBa_B0012 terminator 3278..3318 /label=BBa_B0012 terminator 3190..3318 /label=BBa_B0015 ORIGIN 1 tttacagcta gctcagtcct agggactgtg ctagctacta gagaaagagg agaaatacta 61 gatgaaacga catctgaata cctgctacag gctggtatgg aatcacatga cgggcgcttt 121 cgtggttgcc tccgaactgg cccgcgcacg gggtaaacgt ggcggtgtgg cggttgcact 181 gtctcttgcc gcagtcacgt cactcccggt gctggctgct gacatcgttg tgcacccggg 241 agaaaccgtg aacggcggaa cactggcaaa tcatgacaac cagattgtct tcggtacgac 301 caacggaatg accatcagta ccgggctgga gtatgggccg gataacgagg ccaataccgg 361 cgggcaatgg gtacaggatg gcggaacagc caacaaaacg actgtcacca gtggtggtct 421 tcagagagtg aaccccggtg gaagtgtctc agacacggtt atcagtgccg gaggcggaca 481 gagccttcag ggacgggctg tgaacaccac gctgaatggt ggcgaacagt ggatgcatga 541 gggggcgata gccacaggaa ccgtcattaa tgataagggc tggcaggtcg tcaagcccgg 601 tacagtggca acggataccg ttgttaatac cggggcggaa gggggaccgg atgcagaaaa 661 cggtgatacc gggcagtttg ttcgcgggga tgccgtacgc acaaccatca ataaaaacgg 721 tcgccagatt gtgagagctg aaggaacggc aaataccact gtggtttatg ccggcggcga 781 ccagactgta catggtcacg cactggatac cacgctgaat gggggatacc agtatgtgca 841 caacggcggt acagcgtctg acactgttgt gaacagtgac ggctggcaga ttgtcaaaaa 901 cgggggtgtg gccgggaata ccaccgttaa tcagaagggc agacttcagg tggacgccgg 961 tggtacagcc acgaatgtca ccctgaagca gggcggcgca ctggttacca gtacggctgc 1021 aaccgttacc ggcataaacc gcctgggagc attctctgtt gtggagggta aagctgataa 1081 tgtcgtactg gaaaatggcg gacgcctgga tgtgctgacc ggacacacag ccactaatac 1141 ccgcgtggat gatggcggaa cgctggatgt ccgcaacggt ggcaccgcca ccaccgtatc 1201 catgggaaat ggcggtgtac tgctggccga ttccggtgcc gctgtcagtg gtacccggag 1261 cgacggaaag gcattcagta tcggaggcgg tcaggcggat gccctgatgc tggaaaaagg 1321 cagttcattc acgctgaacg ccggtgatac ggccacggat accacggtaa atggcggact 1381 gttcaccgcc aggggcggca cactggcggg caccaccacg ctgaataacg gcgccatact 1441 taccctttcc gggaagacgg tgaacaacga taccctgacc atccgtgaag gcgatccact 1501 ccttcaggga ggctctctca ccggtaacgg cagcgtggaa aaatcaggaa gtggcacact 1561 cactgtcagc aacaccacac tcacccagaa agccgtcaac ctgaatgaag gcacgctgac 1621 gctgaacgac agtaccgtca ccacggatgt cattgctcag cgcggtacag ccctgaagct 1681 gaccggcagc actgtgctga acggtgccat tgaccccacg aatgtcactc tcgcctccgg 1741 tgccacctgg aatatccccg ataacgccac ggtgcagtcg gtggtggatg acctcagcca 1801 tgccggacag attcatttca cctccacccg cacagggaag ttcgtaccgg caaccctgaa 1861 agtgaaaaac ctgaacggac agaatggcac catcagcctg cgtgtacgcc cggatatggc 1921 acagaacaat gctgacagac tggtcattga cggcggcagg gcaaccggaa aaaccatcct 1981 gaacctggtg aacgccggca acagtgcgtc ggggctggcg accagcggta agggtattca 2041 ggtggtggaa gccattaacg gtgccaccac ggaggaaggg gcctttgtcc aggggaacag 2101 gcttcaggcc ggtgccttta actactccct caaccgggac agtgatgaga gctggtatct 2161 gcgcagtgaa aatgcttatc gtgcagaagt ccccctgtat gcctccatgc tgacacaggc 2221 aatggactat gaccggattg tggcaggctc ccgcagccat cagaccggtg taaatggtga 2281 aaacaacagc gtccgtctca gcattcaggg cggtcatctc ggtcacgata acaatggcgg 2341 tattgcccgt ggggccacgc cggaaagcag cggcagctat ggattcgtcc gtctggaggg 2401 tgacctgatg agaacagagg ttgccggtat gtctgtgacc gcgggggtat atggtgctgc 2461 tggccattct tccgttgatg ttaaggatga tgacggctcc cgtgccggca cggtccggga 2521 tgatgccggc agcctgggcg gatacctgaa tctggtacac acgtcctccg gcctgtgggc 2581 tgacattgtg gcacagggaa cccgccacag catgaaagcg tcatcggaca ataacgactt 2641 ccgcgcccgg ggctggggct ggctgggctc actggaaacc ggtctgccct tcagtatcac 2701 tgacaacctg atgctggagc cacaacttca gtatacctgg cagggacttt ccctggatga 2761 cggtaaggac aacgccgatt atgtgaagtt cgggcatggc agtgcacaac atgtgcgtgc 2821 cggtttccgt ctgggcagcc acaacgatat gacctttggc gaaggcacct catcccgtgc 2881 ccccctgcgt gacagtgcaa aacacagtgt gagtgaatta ccggtgaact ggtgggtaca 2941 gccttctgtt atccgcacct tcagcccccg gggagatatg cgtgtgggga cttccacagc 3001 aggcagcggg atgacgttct ctccctcaca gaatggcaca tcactggacc ttcaggccgg 3061 actggaagcc cgtgtccggg aaaatatcac cctgggcgtt caggccggtt atgcccacag 3121 cgtcagcggc agcagcgctg aagggtataa cggtcaggcc acactgaatg tgaccttctg 3181 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 3241 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 3301 ggcctttctg cgtttata //