LOCUS BBa_K317027 3391 bp DNA linear 04-Nov-2024 DEFINITION OmpR positive promoter(BBa_R0082)-RBS-Antigen 43-Double terminator ACCESSION BBa_K317027 VERSION BBa_K317027.1 FEATURES Location/Qualifiers misc_feature 13..30 /label=C1 OmpR misc_feature 34..51 /label=C2 OmpR misc_feature 54..71 /label=C3 OmpR promoter 75..80 /label=-35 promoter 98..103 /label=-10 promoter 1..108 /label=BBa_R0082 misc_feature 121..124 /label=conserved RBS 117..128 /label=BBa_B0034 CDS 135..3254 /label=BBa_K317008 stem_loop 3274..3317 /label=stem_loop misc_feature 3263..3342 /label=BBa_B0010 terminator 3263..3342 /label=BBa_B0010 stem_loop 3358..3377 /label=T7 TE misc_feature 3384..3384 /label=stop polyA_site 3378..3391 /label=polya misc_feature 3351..3391 /label=BBa_B0012 terminator 3351..3391 /label=BBa_B0012 terminator 3263..3391 /label=BBa_B0015 ORIGIN 1 tcccttgcat ttacattttg aaacatctat agcgataaat gaaacatctt aaaagtttta 61 gtatcatatt cgtgttggat tattctgcat ttttggggag aatggactta ctagagaaag 121 aggagaaata ctagatgaaa cgacatctga atacctgcta caggctggta tggaatcaca 181 tgacgggcgc tttcgtggtt gcctccgaac tggcccgcgc acggggtaaa cgtggcggtg 241 tggcggttgc actgtctctt gccgcagtca cgtcactccc ggtgctggct gctgacatcg 301 ttgtgcaccc gggagaaacc gtgaacggcg gaacactggc aaatcatgac aaccagattg 361 tcttcggtac gaccaacgga atgaccatca gtaccgggct ggagtatggg ccggataacg 421 aggccaatac cggcgggcaa tgggtacagg atggcggaac agccaacaaa acgactgtca 481 ccagtggtgg tcttcagaga gtgaaccccg gtggaagtgt ctcagacacg gttatcagtg 541 ccggaggcgg acagagcctt cagggacggg ctgtgaacac cacgctgaat ggtggcgaac 601 agtggatgca tgagggggcg atagccacag gaaccgtcat taatgataag ggctggcagg 661 tcgtcaagcc cggtacagtg gcaacggata ccgttgttaa taccggggcg gaagggggac 721 cggatgcaga aaacggtgat accgggcagt ttgttcgcgg ggatgccgta cgcacaacca 781 tcaataaaaa cggtcgccag attgtgagag ctgaaggaac ggcaaatacc actgtggttt 841 atgccggcgg cgaccagact gtacatggtc acgcactgga taccacgctg aatgggggat 901 accagtatgt gcacaacggc ggtacagcgt ctgacactgt tgtgaacagt gacggctggc 961 agattgtcaa aaacgggggt gtggccggga ataccaccgt taatcagaag ggcagacttc 1021 aggtggacgc cggtggtaca gccacgaatg tcaccctgaa gcagggcggc gcactggtta 1081 ccagtacggc tgcaaccgtt accggcataa accgcctggg agcattctct gttgtggagg 1141 gtaaagctga taatgtcgta ctggaaaatg gcggacgcct ggatgtgctg accggacaca 1201 cagccactaa tacccgcgtg gatgatggcg gaacgctgga tgtccgcaac ggtggcaccg 1261 ccaccaccgt atccatggga aatggcggtg tactgctggc cgattccggt gccgctgtca 1321 gtggtacccg gagcgacgga aaggcattca gtatcggagg cggtcaggcg gatgccctga 1381 tgctggaaaa aggcagttca ttcacgctga acgccggtga tacggccacg gataccacgg 1441 taaatggcgg actgttcacc gccaggggcg gcacactggc gggcaccacc acgctgaata 1501 acggcgccat acttaccctt tccgggaaga cggtgaacaa cgataccctg accatccgtg 1561 aaggcgatcc actccttcag ggaggctctc tcaccggtaa cggcagcgtg gaaaaatcag 1621 gaagtggcac actcactgtc agcaacacca cactcaccca gaaagccgtc aacctgaatg 1681 aaggcacgct gacgctgaac gacagtaccg tcaccacgga tgtcattgct cagcgcggta 1741 cagccctgaa gctgaccggc agcactgtgc tgaacggtgc cattgacccc acgaatgtca 1801 ctctcgcctc cggtgccacc tggaatatcc ccgataacgc cacggtgcag tcggtggtgg 1861 atgacctcag ccatgccgga cagattcatt tcacctccac ccgcacaggg aagttcgtac 1921 cggcaaccct gaaagtgaaa aacctgaacg gacagaatgg caccatcagc ctgcgtgtac 1981 gcccggatat ggcacagaac aatgctgaca gactggtcat tgacggcggc agggcaaccg 2041 gaaaaaccat cctgaacctg gtgaacgccg gcaacagtgc gtcggggctg gcgaccagcg 2101 gtaagggtat tcaggtggtg gaagccatta acggtgccac cacggaggaa ggggcctttg 2161 tccaggggaa caggcttcag gccggtgcct ttaactactc cctcaaccgg gacagtgatg 2221 agagctggta tctgcgcagt gaaaatgctt atcgtgcaga agtccccctg tatgcctcca 2281 tgctgacaca ggcaatggac tatgaccgga ttgtggcagg ctcccgcagc catcagaccg 2341 gtgtaaatgg tgaaaacaac agcgtccgtc tcagcattca gggcggtcat ctcggtcacg 2401 ataacaatgg cggtattgcc cgtggggcca cgccggaaag cagcggcagc tatggattcg 2461 tccgtctgga gggtgacctg atgagaacag aggttgccgg tatgtctgtg accgcggggg 2521 tatatggtgc tgctggccat tcttccgttg atgttaagga tgatgacggc tcccgtgccg 2581 gcacggtccg ggatgatgcc ggcagcctgg gcggatacct gaatctggta cacacgtcct 2641 ccggcctgtg ggctgacatt gtggcacagg gaacccgcca cagcatgaaa gcgtcatcgg 2701 acaataacga cttccgcgcc cggggctggg gctggctggg ctcactggaa accggtctgc 2761 ccttcagtat cactgacaac ctgatgctgg agccacaact tcagtatacc tggcagggac 2821 tttccctgga tgacggtaag gacaacgccg attatgtgaa gttcgggcat ggcagtgcac 2881 aacatgtgcg tgccggtttc cgtctgggca gccacaacga tatgaccttt ggcgaaggca 2941 cctcatcccg tgcccccctg cgtgacagtg caaaacacag tgtgagtgaa ttaccggtga 3001 actggtgggt acagccttct gttatccgca ccttcagccc ccggggagat atgcgtgtgg 3061 ggacttccac agcaggcagc gggatgacgt tctctccctc acagaatggc acatcactgg 3121 accttcaggc cggactggaa gcccgtgtcc gggaaaatat caccctgggc gttcaggccg 3181 gttatgccca cagcgtcagc ggcagcagcg ctgaagggta taacggtcag gccacactga 3241 atgtgacctt ctgatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac 3301 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc 3361 tcaccttcgg gtgggccttt ctgcgtttat a //