LOCUS       BBa_K317032             1785 bp    DNA     linear       22-Dec-2024
DEFINITION  IPTG regulated T4 Lysis device
ACCESSION   BBa_K317032
VERSION     BBa_K317032.1  
FEATURES             Location/Qualifiers
     CDS             17..673
                     /label=T4 holin
     CDS             1..676
                     /label=BBa_K112805
     CDS             701..1195
                     /label=T4 Endolysin
     CDS             685..1198
                     /label=BBa_K112806
     stem_loop       1218..1261
                     /label=stem_loop
     misc_feature    1207..1286
                     /label=BBa_B0010
     terminator      1207..1286
                     /label=BBa_B0010
     stem_loop       1302..1321
                     /label=T7 TE
     misc_feature    1328..1328
                     /label=stop
     polyA_site      1322..1335
                     /label=polya
     misc_feature    1295..1335
                     /label=BBa_B0012
     terminator      1295..1335
                     /label=BBa_B0012
     promoter        1344..1378
                     /label=BBa_J23116
     CDS             1401..1694
                     /label=T4 antiholin
     CDS             1387..1697
                     /label=BBa_K112807
     stem_loop       1717..1760
                     /label=stem_loop
     misc_feature    1706..1785
                     /label=BBa_B0010
     terminator      1706..1785
                     /label=BBa_B0010
     misc_feature    1..1785
                     /label=BBa_K112808
ORIGIN
        1 cttaaaagga gggtctatgg cagcacctag aatatcattt tcgccctctg atattctatt
       61 tggtgttctc gatcgcttgt tcaaagataa cgctaccggg aaggttcttg cttcccgggt
      121 agctgtcgta attcttttgt ttataatggc gattgtttgg tataggggag atagtttctt
      181 tgagtactat aagcaatcaa agtatgaaac atacagtgaa attattgaaa aggaaagaac
      241 tgcacgcttt gaatctgtcg ccctggaaca actccagata gttcatatat catctgaggc
      301 agactttagt gcggtgtatt ctttccgccc taaaaactta aactattttg ttgatattat
      361 agcatacgaa ggaaaattac cttcaacaat aagtgaaaaa tcacttggag gatatcctgt
      421 tgataaaact atggatgaat atacagttca tttaaatgga cgtcattatt attccaactc
      481 aaaatttgct tttttaccaa ctaaaaagcc tactcccgaa ataaactaca tgtacagttg
      541 tccatatttt aatttggata atatctatgc tggaacgata accatgtact ggtatagaaa
      601 tgatcatata agtaatgacc gccttgaatc aatatgtgct caggcggcca gaatattagg
      661 aagggctaaa taattatact agagatactt aggaggtatt atgaatatat ttgaaatgtt
      721 acgtatagat gaaggtctta gacttaaaat ctataaagac acagaaggct attacactat
      781 tggcatcggt catttgctta caaaaagtcc atcacttaat gctgctaaat ctgaattaga
      841 taaagctatt gggcgtaatt gcaatggtgt aattacaaaa gatgaggctg aaaaactctt
      901 taatcaggat gttgatgctg ctgttcgcgg aatcctgaga aatgctaaat taaaaccggt
      961 ttatgattct cttgatgcgg ttcgtcgctg tgcattgatt aatatggttt tccaaatggg
     1021 agaaaccggt gtggcaggat ttactaactc tttacgtatg cttcaacaaa aacgctggga
     1081 tgaagcagca gttaacttag ctaaaagtag atggtataat caaacaccta atcgcgcaaa
     1141 acgagtcatt acaacgttta gaactggcac ttgggacgcg tataaaaatc tataaagcta
     1201 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1261 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     1321 ctttctgcgt ttatatacta gagttgacag ctagctcagt cctagggact atgctagcta
     1381 ctagaggata ggaggccttt atggccttaa aagcaacagc actttttgcc atgctaggat
     1441 tgtcatttgt tttatctcca tcgattgaag cgaatgtcga tcctcatttt gataaattta
     1501 tggaatctgg tattaggcac gtttatatgc tttttgaaaa taaaagcgta gaatcgtctg
     1561 aacaattcta tagttttatg agaacgacct ataaaaatga cccgtgctct tctgattttg
     1621 aatgtataga gcgaggcgcg gagatggcac aatcatacgc tagaattatg aacattaaat
     1681 tggagactga atgaaattac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa
     1741 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctc
//