LOCUS       BBa_K317038             2792 bp    DNA     linear       22-Nov-2024
DEFINITION  RBS-LuxR-Double term-Plux-Lysis
ACCESSION   BBa_K317038
VERSION     BBa_K317038.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    19..20
                     /label=prefix
     misc_feature    192..192
                     /label=T
     misc_feature    510..510
                     /label=A
     CDS             19..768
                     /label=luxR
     misc_feature    19..774
                     /label=BBa_C0062
     misc_feature    775..799
                     /label=Help:Barcodes
     CDS             19..774
                     /label=BBa_C0062
     stem_loop       819..862
                     /label=stem_loop
     misc_feature    808..887
                     /label=BBa_B0010
     terminator      808..887
                     /label=BBa_B0010
     stem_loop       903..922
                     /label=T7 TE
     misc_feature    929..929
                     /label=stop
     polyA_site      923..936
                     /label=polya
     misc_feature    896..936
                     /label=BBa_B0012
     terminator      896..936
                     /label=BBa_B0012
     terminator      808..936
                     /label=BBa_B0015
     misc_feature    945..964
                     /label=LuxR/HSL
     promoter        964..969
                     /label=-35
     promoter        986..991
                     /label=-10
     misc_feature    997..997
                     /label=start
     misc_feature    945..999
                     /label=BBa_R0062
     promoter        945..999
                     /label=BBa_R0062
     CDS             1024..1680
                     /label=T4 holin
     CDS             1008..1683
                     /label=BBa_K112805
     CDS             1708..2202
                     /label=T4 Endolysin
     CDS             1692..2205
                     /label=BBa_K112806
     stem_loop       2225..2268
                     /label=stem_loop
     misc_feature    2214..2293
                     /label=BBa_B0010
     terminator      2214..2293
                     /label=BBa_B0010
     stem_loop       2309..2328
                     /label=T7 TE
     misc_feature    2335..2335
                     /label=stop
     polyA_site      2329..2342
                     /label=polya
     misc_feature    2302..2342
                     /label=BBa_B0012
     terminator      2302..2342
                     /label=BBa_B0012
     promoter        2351..2385
                     /label=BBa_J23116
     CDS             2408..2701
                     /label=T4 antiholin
     CDS             2394..2704
                     /label=BBa_K112807
     stem_loop       2724..2767
                     /label=stem_loop
     misc_feature    2713..2792
                     /label=BBa_B0010
     terminator      2713..2792
                     /label=BBa_B0010
     misc_feature    1008..2792
                     /label=BBa_K112808
ORIGIN
        1 aaagaggaga aatactagat gaaaaacata aatgccgacg acacatacag aataattaat
       61 aaaattaaag cttgtagaag caataatgat attaatcaat gcttatctga tatgactaaa
      121 atggtacatt gtgaatatta tttactcgcg atcatttatc ctcattctat ggttaaatct
      181 gatatttcaa tcctagataa ttaccctaaa aaatggaggc aatattatga tgacgctaat
      241 ttaataaaat atgatcctat agtagattat tctaactcca atcattcacc aattaattgg
      301 aatatatttg aaaacaatgc tgtaaataaa aaatctccaa atgtaattaa agaagcgaaa
      361 acatcaggtc ttatcactgg gtttagtttc cctattcata cggctaacaa tggcttcgga
      421 atgcttagtt ttgcacattc agaaaaagac aactatatag atagtttatt tttacatgcg
      481 tgtatgaaca taccattaat tgttccttct ctagttgata attatcgaaa aataaatata
      541 gcaaataata aatcaaacaa cgatttaacc aaaagagaaa aagaatgttt agcgtgggca
      601 tgcgaaggaa aaagctcttg ggatatttca aaaatattag gttgcagtga gcgtactgtc
      661 actttccatt taaccaatgc gcaaatgaaa ctcaatacaa caaaccgctg ccaaagtatt
      721 tctaaagcaa ttttaacagg agcaattgat tgcccatact ttaaaaatta ataacactga
      781 tagtgctagt gtagatcact actagagcca ggcatcaaat aaaacgaaag gctcagtcga
      841 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
      901 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagacctgt aggatcgtac
      961 aggtttacgc aagaaaatgg tttgttatag tcgaataaat actagagctt aaaaggaggg
     1021 tctatggcag cacctagaat atcattttcg ccctctgata ttctatttgg tgttctcgat
     1081 cgcttgttca aagataacgc taccgggaag gttcttgctt cccgggtagc tgtcgtaatt
     1141 cttttgttta taatggcgat tgtttggtat aggggagata gtttctttga gtactataag
     1201 caatcaaagt atgaaacata cagtgaaatt attgaaaagg aaagaactgc acgctttgaa
     1261 tctgtcgccc tggaacaact ccagatagtt catatatcat ctgaggcaga ctttagtgcg
     1321 gtgtattctt tccgccctaa aaacttaaac tattttgttg atattatagc atacgaagga
     1381 aaattacctt caacaataag tgaaaaatca cttggaggat atcctgttga taaaactatg
     1441 gatgaatata cagttcattt aaatggacgt cattattatt ccaactcaaa atttgctttt
     1501 ttaccaacta aaaagcctac tcccgaaata aactacatgt acagttgtcc atattttaat
     1561 ttggataata tctatgctgg aacgataacc atgtactggt atagaaatga tcatataagt
     1621 aatgaccgcc ttgaatcaat atgtgctcag gcggccagaa tattaggaag ggctaaataa
     1681 ttatactaga gatacttagg aggtattatg aatatatttg aaatgttacg tatagatgaa
     1741 ggtcttagac ttaaaatcta taaagacaca gaaggctatt acactattgg catcggtcat
     1801 ttgcttacaa aaagtccatc acttaatgct gctaaatctg aattagataa agctattggg
     1861 cgtaattgca atggtgtaat tacaaaagat gaggctgaaa aactctttaa tcaggatgtt
     1921 gatgctgctg ttcgcggaat cctgagaaat gctaaattaa aaccggttta tgattctctt
     1981 gatgcggttc gtcgctgtgc attgattaat atggttttcc aaatgggaga aaccggtgtg
     2041 gcaggattta ctaactcttt acgtatgctt caacaaaaac gctgggatga agcagcagtt
     2101 aacttagcta aaagtagatg gtataatcaa acacctaatc gcgcaaaacg agtcattaca
     2161 acgtttagaa ctggcacttg ggacgcgtat aaaaatctat aaagctacta gagccaggca
     2221 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     2281 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
     2341 tatactagag ttgacagcta gctcagtcct agggactatg ctagctacta gaggatagga
     2401 ggcctttatg gccttaaaag caacagcact ttttgccatg ctaggattgt catttgtttt
     2461 atctccatcg attgaagcga atgtcgatcc tcattttgat aaatttatgg aatctggtat
     2521 taggcacgtt tatatgcttt ttgaaaataa aagcgtagaa tcgtctgaac aattctatag
     2581 ttttatgaga acgacctata aaaatgaccc gtgctcttct gattttgaat gtatagagcg
     2641 aggcgcggag atggcacaat catacgctag aattatgaac attaaattgg agactgaatg
     2701 aaattactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     2761 gttttatctg ttgtttgtcg gtgaacgctc tc
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