LOCUS       BBa_K317039             2835 bp    DNA     linear       22-Nov-2024
DEFINITION  Pconst.(H)-RBS-LuxR-Double term-Plux-Lysis
ACCESSION   BBa_K317039
VERSION     BBa_K317039.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23102
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    62..63
                     /label=prefix
     misc_feature    235..235
                     /label=T
     misc_feature    553..553
                     /label=A
     CDS             62..811
                     /label=luxR
     misc_feature    62..817
                     /label=BBa_C0062
     misc_feature    818..842
                     /label=Help:Barcodes
     CDS             62..817
                     /label=BBa_C0062
     stem_loop       862..905
                     /label=stem_loop
     misc_feature    851..930
                     /label=BBa_B0010
     terminator      851..930
                     /label=BBa_B0010
     stem_loop       946..965
                     /label=T7 TE
     misc_feature    972..972
                     /label=stop
     polyA_site      966..979
                     /label=polya
     misc_feature    939..979
                     /label=BBa_B0012
     terminator      939..979
                     /label=BBa_B0012
     terminator      851..979
                     /label=BBa_B0015
     misc_feature    988..1007
                     /label=LuxR/HSL
     promoter        1007..1012
                     /label=-35
     promoter        1029..1034
                     /label=-10
     misc_feature    1040..1040
                     /label=start
     misc_feature    988..1042
                     /label=BBa_R0062
     promoter        988..1042
                     /label=BBa_R0062
     CDS             1067..1723
                     /label=T4 holin
     CDS             1051..1726
                     /label=BBa_K112805
     CDS             1751..2245
                     /label=T4 Endolysin
     CDS             1735..2248
                     /label=BBa_K112806
     stem_loop       2268..2311
                     /label=stem_loop
     misc_feature    2257..2336
                     /label=BBa_B0010
     terminator      2257..2336
                     /label=BBa_B0010
     stem_loop       2352..2371
                     /label=T7 TE
     misc_feature    2378..2378
                     /label=stop
     polyA_site      2372..2385
                     /label=polya
     misc_feature    2345..2385
                     /label=BBa_B0012
     terminator      2345..2385
                     /label=BBa_B0012
     promoter        2394..2428
                     /label=BBa_J23116
     CDS             2451..2744
                     /label=T4 antiholin
     CDS             2437..2747
                     /label=BBa_K112807
     stem_loop       2767..2810
                     /label=stem_loop
     misc_feature    2756..2835
                     /label=BBa_B0010
     terminator      2756..2835
                     /label=BBa_B0010
     misc_feature    1051..2835
                     /label=BBa_K112808
ORIGIN
        1 ttgacagcta gctcagtcct aggtactgtg ctagctacta gagaaagagg agaaatacta
       61 gatgaaaaac ataaatgccg acgacacata cagaataatt aataaaatta aagcttgtag
      121 aagcaataat gatattaatc aatgcttatc tgatatgact aaaatggtac attgtgaata
      181 ttatttactc gcgatcattt atcctcattc tatggttaaa tctgatattt caatcctaga
      241 taattaccct aaaaaatgga ggcaatatta tgatgacgct aatttaataa aatatgatcc
      301 tatagtagat tattctaact ccaatcattc accaattaat tggaatatat ttgaaaacaa
      361 tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg aaaacatcag gtcttatcac
      421 tgggtttagt ttccctattc atacggctaa caatggcttc ggaatgctta gttttgcaca
      481 ttcagaaaaa gacaactata tagatagttt atttttacat gcgtgtatga acataccatt
      541 aattgttcct tctctagttg ataattatcg aaaaataaat atagcaaata ataaatcaaa
      601 caacgattta accaaaagag aaaaagaatg tttagcgtgg gcatgcgaag gaaaaagctc
      661 ttgggatatt tcaaaaatat taggttgcag tgagcgtact gtcactttcc atttaaccaa
      721 tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt atttctaaag caattttaac
      781 aggagcaatt gattgcccat actttaaaaa ttaataacac tgatagtgct agtgtagatc
      841 actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      901 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
      961 gggcctttct gcgtttatat actagagacc tgtaggatcg tacaggttta cgcaagaaaa
     1021 tggtttgtta tagtcgaata aatactagag cttaaaagga gggtctatgg cagcacctag
     1081 aatatcattt tcgccctctg atattctatt tggtgttctc gatcgcttgt tcaaagataa
     1141 cgctaccggg aaggttcttg cttcccgggt agctgtcgta attcttttgt ttataatggc
     1201 gattgtttgg tataggggag atagtttctt tgagtactat aagcaatcaa agtatgaaac
     1261 atacagtgaa attattgaaa aggaaagaac tgcacgcttt gaatctgtcg ccctggaaca
     1321 actccagata gttcatatat catctgaggc agactttagt gcggtgtatt ctttccgccc
     1381 taaaaactta aactattttg ttgatattat agcatacgaa ggaaaattac cttcaacaat
     1441 aagtgaaaaa tcacttggag gatatcctgt tgataaaact atggatgaat atacagttca
     1501 tttaaatgga cgtcattatt attccaactc aaaatttgct tttttaccaa ctaaaaagcc
     1561 tactcccgaa ataaactaca tgtacagttg tccatatttt aatttggata atatctatgc
     1621 tggaacgata accatgtact ggtatagaaa tgatcatata agtaatgacc gccttgaatc
     1681 aatatgtgct caggcggcca gaatattagg aagggctaaa taattatact agagatactt
     1741 aggaggtatt atgaatatat ttgaaatgtt acgtatagat gaaggtctta gacttaaaat
     1801 ctataaagac acagaaggct attacactat tggcatcggt catttgctta caaaaagtcc
     1861 atcacttaat gctgctaaat ctgaattaga taaagctatt gggcgtaatt gcaatggtgt
     1921 aattacaaaa gatgaggctg aaaaactctt taatcaggat gttgatgctg ctgttcgcgg
     1981 aatcctgaga aatgctaaat taaaaccggt ttatgattct cttgatgcgg ttcgtcgctg
     2041 tgcattgatt aatatggttt tccaaatggg agaaaccggt gtggcaggat ttactaactc
     2101 tttacgtatg cttcaacaaa aacgctggga tgaagcagca gttaacttag ctaaaagtag
     2161 atggtataat caaacaccta atcgcgcaaa acgagtcatt acaacgttta gaactggcac
     2221 ttgggacgcg tataaaaatc tataaagcta ctagagccag gcatcaaata aaacgaaagg
     2281 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     2341 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagttgacag
     2401 ctagctcagt cctagggact atgctagcta ctagaggata ggaggccttt atggccttaa
     2461 aagcaacagc actttttgcc atgctaggat tgtcatttgt tttatctcca tcgattgaag
     2521 cgaatgtcga tcctcatttt gataaattta tggaatctgg tattaggcac gtttatatgc
     2581 tttttgaaaa taaaagcgta gaatcgtctg aacaattcta tagttttatg agaacgacct
     2641 ataaaaatga cccgtgctct tctgattttg aatgtataga gcgaggcgcg gagatggcac
     2701 aatcatacgc tagaattatg aacattaaat tggagactga atgaaattac tagagccagg
     2761 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     2821 tcggtgaacg ctctc
//