##gff-version 3 ##sequence-region BBa_K318000 1 4395 BBa_K318000 . terminator 2379 2417 . + 0 ID=BBa_B1006;Name=BBa_B1006 BBa_K318000 . polyA_site 2379 2387 . + 0 ID=annotation1898431;Name=PolyA;Parent=BBa_B1006 BBa_K318000 . engineered_region 2379 2417 . + 0 ID=annotation1898428;Name=B1006;Parent=BBa_B1006 BBa_K318000 . polyA_site 2410 2417 . + 0 ID=annotation1898430;Name=PolyA;Parent=BBa_B1006 BBa_K318000 . stem_loop 2388 2409 . + 0 ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006 BBa_K318000 . terminator 1 39 . + 0 ID=BBa_B1006;Name=BBa_B1006 BBa_K318000 . polyA_site 1 9 . + 0 ID=annotation1898431;Name=PolyA;Parent=BBa_B1006 BBa_K318000 . engineered_region 1 39 . + 0 ID=annotation1898428;Name=B1006;Parent=BBa_B1006 BBa_K318000 . polyA_site 32 39 . + 0 ID=annotation1898430;Name=PolyA;Parent=BBa_B1006 BBa_K318000 . stem_loop 10 31 . + 0 ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006 BBa_K318000 . CDS 2507 3079 . + 0 ID=BBa_J31000;Name=BBa_J31000 BBa_K318000 . sequence_alteration 2578 2578 . + 0 ID=annotation1880460;Name=Mutation to delete the SpeI site;Parent=BBa_J31000 BBa_K318000 . primer_binding_site 2507 2509 . + 0 ID=annotation1910568;Name=Fwd primer J31001;Parent=BBa_J31000 BBa_K318000 . sequence_alteration 2576 2581 . + 0 ID=annotation1880459;Name=Former SpeI site;Parent=BBa_J31000 BBa_K318000 . primer_binding_site 3057 3076 . + 0 ID=annotation1910569;Name=Rev primer J31001;Parent=BBa_J31000 BBa_K318000 . CDS 2507 3079 . + 0 ID=annotation1884987;Name=Hin sequence;Parent=BBa_J31000 BBa_K318000 . promoter 3135 3169 . + 0 ID=BBa_J23100;Name=BBa_J23100 BBa_K318000 . engineered_region 2426 2500 . + 0 ID=BBa_K200021;Name=BBa_K200021 BBa_K318000 . promoter 2426 2479 . + 0 ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_K200021 BBa_K318000 . promoter 2445 2450 . + 0 ID=annotation2000;Name=-35;Parent=BBa_R0011 BBa_K318000 . promoter 2468 2473 . + 0 ID=annotation2002;Name=-10;Parent=BBa_R0011 BBa_K318000 . operator 2451 2467 . + 0 ID=annotation2001;Name=lac O1;Parent=BBa_R0011 BBa_K318000 . engineered_region 2426 2479 . + 0 ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011 BBa_K318000 . operator 2428 2444 . + 0 ID=annotation1999;Name=lac O1;Parent=BBa_R0011 BBa_K318000 . ribosome_entry_site 2489 2500 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K200021 BBa_K318000 . conserved_region 2493 2496 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_K318000 . terminator 4357 4395 . + 0 ID=BBa_B1006;Name=BBa_B1006 BBa_K318000 . polyA_site 4357 4365 . + 0 ID=annotation1898431;Name=PolyA;Parent=BBa_B1006 BBa_K318000 . engineered_region 4357 4395 . + 0 ID=annotation1898428;Name=B1006;Parent=BBa_B1006 BBa_K318000 . polyA_site 4388 4395 . + 0 ID=annotation1898430;Name=PolyA;Parent=BBa_B1006 BBa_K318000 . stem_loop 4366 4387 . + 0 ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006 BBa_K318000 . terminator 3088 3126 . + 0 ID=BBa_B1006;Name=BBa_B1006 BBa_K318000 . polyA_site 3088 3096 . + 0 ID=annotation1898431;Name=PolyA;Parent=BBa_B1006 BBa_K318000 . engineered_region 3088 3126 . + 0 ID=annotation1898428;Name=B1006;Parent=BBa_B1006 BBa_K318000 . polyA_site 3119 3126 . + 0 ID=annotation1898430;Name=PolyA;Parent=BBa_B1006 BBa_K318000 . stem_loop 3097 3118 . + 0 ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006 BBa_K318000 . terminator 2332 2370 . + 0 ID=BBa_B1006;Name=BBa_B1006 BBa_K318000 . polyA_site 2332 2340 . + 0 ID=annotation1898431;Name=PolyA;Parent=BBa_B1006 BBa_K318000 . engineered_region 2332 2370 . + 0 ID=annotation1898428;Name=B1006;Parent=BBa_B1006 BBa_K318000 . polyA_site 2363 2370 . + 0 ID=annotation1898430;Name=PolyA;Parent=BBa_B1006 BBa_K318000 . stem_loop 2341 2362 . + 0 ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006 BBa_K318000 . engineered_region 48 2323 . + 0 ID=BBa_I718008;Name=BBa_I718008 BBa_K318000 . CDS 1287 2323 . + 0 ID=BBa_J61047;Name=BBa_J61047;Parent=BBa_I718008 BBa_K318000 . ribosome_entry_site 1266 1280 . + 0 ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_I718008 BBa_K318000 . ribosome_entry_site 1273 1277 . + 0 ID=annotation1702;Name=RBS;Parent=BBa_B0030 BBa_K318000 . ribosome_entry_site 1266 1280 . + 0 ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030 BBa_K318000 . engineered_region 1266 1280 . + 0 ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030 BBa_K318000 . promoter 48 1257 . + 0 ID=BBa_I0500;Name=BBa_I0500;Parent=BBa_I718008 BBa_K318000 . promoter 1090 1161 . + 0 ID=annotation1498842;Name=PC;Parent=BBa_I0500 BBa_K318000 . promoter 1127 1257 . + 0 ID=annotation1498841;Name=Pbad;Parent=BBa_I0500 BBa_K318000 . sequence_feature 48 1126 . + 0 ID=annotation1498843;Name=AraC;Parent=BBa_I0500 BBa_K318000 . engineered_region 3178 4348 . + 0 ID=BBa_S03520;Name=BBa_S03520 BBa_K318000 . ribosome_entry_site 3178 3189 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03520 BBa_K318000 . conserved_region 3182 3185 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_K318000 . CDS 3196 4323 . + 0 ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_S03520 BBa_K318000 . engineered_region 3196 4323 . + 0 ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012 BBa_K318000 . tag 4285 4323 . + 0 ID=annotation1722;Name=LVA;Parent=BBa_C0012 BBa_K318000 . engineered_tag 4324 4348 . + 0 ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012 BBa_K318000 . CDS 3196 4323 . + 0 ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012 >BBa_K318000 aaaaaaaaaccccgcccctgacagggcggggtttttttttactagagttatgacaacttgacggctacatcattcactt tttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatc gtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgt tggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaa catgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccg attatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgcc agcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcg cttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacg aaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagc aaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaa tataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccac cagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattc agagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaac cggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtcta taatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataaga ttagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactaga gattaaagaggagaaatactagatgtccaatttactgaccgtacaccaaaatttgcctgcattaccggtcgatgcaacg agtgatgaggttcgcaagaacctgatggacatgttcagggatcgccaggcgttttctgagcatacctggaaaatgcttc tgtccgtttgccggtcgtgggcggcatggtgcaagttgaataaccggaaatggtttcccgcagaacctgaagatgttcg cgattatcttctatatcttcaggcgcgcggtctggcagtaaaaactatccagcaacatttgggccagctaaacatgctt catcgtcggtccgggctgccacgaccaagtgacagcaatgctgtttcactggttatgcggcggatccgaaaagaaaacg ttgatgccggtgaacgtgcaaaacaggctctagcgttcgaacgcactgatttcgaccaggttcgttcactcatggaaaa tagcgatcgctgccaggatatacgtaatctggcatttctggggattgcttataacaccctgttacgtatagccgaaatt gccaggatcagggttaaagatatctcacgtactgacggtgggagaatgttaatccatattggcagaacgaaaacgctgg ttagcaccgcaggtgtagagaaggcacttagcctgggggtaactaaactggtcgagcgatggatttccgtctctggtgt agctgatgatccgaataactacctgttttgccgggtcagaaaaaatggtgttgccgcgccatctgccaccagccagcta tcaactcgcgccctggaagggatttttgaagcaactcatcgattgatttacggcgctaaggatgactctggtcagagat acctggcctggtctggacacagtgcccgtgtcggagccgcgcgagatatggcccgcgctggagtttcaataccggagat catgcaagctggtggctggaccaatgtaaatattgtcatgaactatatccgtaacctggatagtgaaacaggggcaatg gtgcgcctgctggaagatggcgattaagaatttactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt tactagagaaaaaaaaaccccgcccctgacagggcggggtttttttttactagagaattgtgagcggataacaattgac attgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatggctactattgggtatattc gggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttaccagtgcaaattgtgaccgcatttttgaaga ccgtatcagtggcaagattgcaaaccgccccggcctgaaacgggcgttaaagtatgtaaataaaggcgatactcttgtc gtctggaaattagacagactgggccgtagcgtgaaaaatctggtggcgttaatatcagaattacatgaacgtggagctc acttccattctttaaccgatagtattgataccagtagcgcgatggggcgattcttttttcatgtaatgtcagcactggc cgagatggagcgagaattaatcgtcgagcgaacccttgccggactggctgccgccagagcgcaaggacgactgggaggg cgccctcgggcgatcaacaaacatgaacaggaacagattagtcggctattagagaaaggccatcctcggcagcaattag ctattatttttggtattggcgtatccaccttatacagatactttccggcaagcagtataaaaaaacgaatgaattaata ctagagaaaaaaaaaccccgcccctgacagggcggggtttttttttactagagttgacggctagctcagtcctaggtac agtgctagctactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagta tgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtg gaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattg gcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactggg tgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaa cgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttc cggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactggg cgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctg cgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgcca tgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagat ggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgat accgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtgg accgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaac caccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcc cgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagat ctctactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt