LOCUS BBa_K318010 2370 bp DNA linear 05-Nov-2024 DEFINITION T + araC + pBad + RBS + Cre + T ACCESSION BBa_K318010 VERSION BBa_K318010.1 FEATURES Location/Qualifiers misc_feature 1..39 /label=B1006 stem_loop 10..31 /label=modified thr terminator polyA_site 32..39 /label=PolyA polyA_site 1..9 /label=PolyA terminator 1..39 /label=BBa_B1006 promoter 1127..1257 /label=Pbad promoter 1090..1161 /label=PC misc_feature 48..1126 /label=AraC promoter 48..1257 /label=BBa_I0500 RBS 1266..1280 /label=RBS-1\Strong RBS 1273..1277 /label=RBS misc_feature 1266..1280 /label=BBa_B0030 RBS 1266..1280 /label=BBa_B0030 CDS 1287..2323 /label=BBa_J61047 misc_feature 2332..2370 /label=B1006 stem_loop 2341..2362 /label=modified thr terminator polyA_site 2363..2370 /label=PolyA polyA_site 2332..2340 /label=PolyA terminator 2332..2370 /label=BBa_B1006 ORIGIN 1 aaaaaaaaac cccgcccctg acagggcggg gttttttttt actagagtta tgacaacttg 61 acggctacat cattcacttt ttcttcacaa ccggcacgga actcgctcgg gctggccccg 121 gtgcattttt taaatacccg cgagaaatag agttgatcgt caaaaccaac attgcgaccg 181 acggtggcga taggcatccg ggtggtgctc aaaagcagct tcgcctggct gatacgttgg 241 tcctcgcgcc agcttaagac gctaatccct aactgctggc ggaaaagatg tgacagacgc 301 gacggcgaca agcaaacatg ctgtgcgacg ctggcgatat caaaattgct gtctgccagg 361 tgatcgctga tgtactgaca agcctcgcgt acccgattat ccatcggtgg atggagcgac 421 tcgttaatcg cttccatgcg ccgcagtaac aattgctcaa gcagatttat cgccagcagc 481 tccgaatagc gcccttcccc ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa 541 tgcggctggt gcgcttcatc cgggcgaaag aaccccgtat tggcaaatat tgacggccag 601 ttaagccatt catgccagta ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg 661 cgagcctccg gatgacgacc gtagtgatga atctctcctg gcgggaacag caaaatatca 721 cccggtcggc aaacaaattc tcgtccctga tttttcacca ccccctgacc gcgaatggtg 781 agattgagaa tataaccttt cattcccagc ggtcggtcga taaaaaaatc gagataaccg 841 ttggcctcaa tcggcgttaa acccgccacc agatgggcat taaacgagta tcccggcagc 901 aggggatcat tttgcgcttc agccatactt ttcatactcc cgccattcag agaagaaacc 961 aattgtccat attgcatcag acattgccgt cactgcgtct tttactggct cttctcgcta 1021 accaaaccgg taaccccgct tattaaaagc attctgtaac aaagcgggac caaagccatg 1081 acaaaaacgc gtaacaaaag tgtctataat cacggcagaa aagtccacat tgattatttg 1141 cacggcgtca cactttgcta tgccatagca tttttatcca taagattagc ggatcctacc 1201 tgacgctttt tatcgcaact ctctactgtt tctccatacc cgtttttttg ggctagctac 1261 tagagattaa agaggagaaa tactagatgt ccaatttact gaccgtacac caaaatttgc 1321 ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg gacatgttca 1381 gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt tgccggtcgt 1441 gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct gaagatgttc 1501 gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaactatc cagcaacatt 1561 tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt gacagcaatg 1621 ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt gaacgtgcaa 1681 aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc atggaaaata 1741 gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat aacaccctgt 1801 tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact gacggtggga 1861 gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt gtagagaagg 1921 cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct ggtgtagctg 1981 atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc gcgccatctg 2041 ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact catcgattga 2101 tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga cacagtgccc 2161 gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag atcatgcaag 2221 ctggtggctg gaccaatgta aatattgtca tgaactatat ccgtaacctg gatagtgaaa 2281 caggggcaat ggtgcgcctg ctggaagatg gcgattaaga atttactaga gaaaaaaaaa 2341 ccccgcccct gacagggcgg ggtttttttt //