LOCUS       BBa_K318011              748 bp    DNA     linear       27-Jan-2026
DEFINITION  T + pLac + RBS + Hin + T
ACCESSION   BBa_K318011
VERSION     BBa_K318011.1  
FEATURES             Location/Qualifiers
     misc_feature    1..39
                     /label=B1006
     stem_loop       10..31
                     /label=modified thr terminator
     polyA_site      32..39
                     /label=PolyA
     polyA_site      1..9
                     /label=PolyA
     terminator      1..39
                     /label=BBa_B1006
     misc_feature    50..66
                     /label=lac O1
     promoter        67..72
                     /label=-35
     misc_feature    73..89
                     /label=lac O1
     promoter        90..95
                     /label=-10
     misc_feature    48..101
                     /label=BBa_R0011
     promoter        48..101
                     /label=BBa_R0011
     misc_feature    115..118
                     /label=conserved
     RBS             111..122
                     /label=BBa_B0034
     misc_feature    198..203
                     /label=Former SpeI site
     misc_feature    200..200
                     /label=Mutation to delete the SpeI site
     CDS             129..701
                     /label=Hin sequence
     primer_bind     129..131
                     /label=Fwd primer J31001
     primer_bind     679..698
                     /label=Rev primer J31001
     CDS             129..701
                     /label=BBa_J31000
     misc_feature    710..748
                     /label=B1006
     stem_loop       719..740
                     /label=modified thr terminator
     polyA_site      741..748
                     /label=PolyA
     polyA_site      710..718
                     /label=PolyA
     terminator      710..748
                     /label=BBa_B1006
ORIGIN
        1 aaaaaaaaac cccgcccctg acagggcggg gttttttttt actagagaat tgtgagcgga
       61 taacaattga cattgtgagc ggataacaag atactgagca catactagag aaagaggaga
      121 aatactagat ggctactatt gggtatattc gggtgtcaac aattgaccaa aatatcgatt
      181 tacagcgtaa tgcgcttacc agtgcaaatt gtgaccgcat ttttgaagac cgtatcagtg
      241 gcaagattgc aaaccgcccc ggcctgaaac gggcgttaaa gtatgtaaat aaaggcgata
      301 ctcttgtcgt ctggaaatta gacagactgg gccgtagcgt gaaaaatctg gtggcgttaa
      361 tatcagaatt acatgaacgt ggagctcact tccattcttt aaccgatagt attgatacca
      421 gtagcgcgat ggggcgattc ttttttcatg taatgtcagc actggccgag atggagcgag
      481 aattaatcgt cgagcgaacc cttgccggac tggctgccgc cagagcgcaa ggacgactgg
      541 gagggcgccc tcgggcgatc aacaaacatg aacaggaaca gattagtcgg ctattagaga
      601 aaggccatcc tcggcagcaa ttagctatta tttttggtat tggcgtatcc accttataca
      661 gatactttcc ggcaagcagt ataaaaaaac gaatgaatta atactagaga aaaaaaaacc
      721 ccgcccctga cagggcgggg tttttttt
//