##gff-version 3
##sequence-region BBa_K318011 1 748
BBa_K318011	.	CDS	129	701	.	+	0	ID=BBa_J31000;Name=BBa_J31000
BBa_K318011	.	primer_binding_site	679	698	.	+	0	ID=annotation1910569;Name=Rev primer J31001;Parent=BBa_J31000
BBa_K318011	.	sequence_alteration	200	200	.	+	0	ID=annotation1880460;Name=Mutation to delete the SpeI site;Parent=BBa_J31000
BBa_K318011	.	primer_binding_site	129	131	.	+	0	ID=annotation1910568;Name=Fwd primer J31001;Parent=BBa_J31000
BBa_K318011	.	sequence_alteration	198	203	.	+	0	ID=annotation1880459;Name=Former SpeI site;Parent=BBa_J31000
BBa_K318011	.	CDS	129	701	.	+	0	ID=annotation1884987;Name=Hin sequence;Parent=BBa_J31000
BBa_K318011	.	terminator	1	39	.	+	0	ID=BBa_B1006;Name=BBa_B1006
BBa_K318011	.	polyA_site	1	9	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K318011	.	polyA_site	32	39	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K318011	.	engineered_region	1	39	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_K318011	.	stem_loop	10	31	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K318011	.	terminator	710	748	.	+	0	ID=BBa_B1006;Name=BBa_B1006
BBa_K318011	.	polyA_site	710	718	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K318011	.	polyA_site	741	748	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K318011	.	engineered_region	710	748	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_K318011	.	stem_loop	719	740	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K318011	.	ribosome_entry_site	111	122	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K318011	.	conserved_region	115	118	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K318011	.	promoter	48	101	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K318011	.	operator	73	89	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K318011	.	operator	50	66	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K318011	.	promoter	90	95	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K318011	.	engineered_region	48	101	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K318011	.	promoter	67	72	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
>BBa_K318011
aaaaaaaaaccccgcccctgacagggcggggtttttttttactagagaattgtgagcggataacaattgacattgtgag
cggataacaagatactgagcacatactagagaaagaggagaaatactagatggctactattgggtatattcgggtgtca
acaattgaccaaaatatcgatttacagcgtaatgcgcttaccagtgcaaattgtgaccgcatttttgaagaccgtatca
gtggcaagattgcaaaccgccccggcctgaaacgggcgttaaagtatgtaaataaaggcgatactcttgtcgtctggaa
attagacagactgggccgtagcgtgaaaaatctggtggcgttaatatcagaattacatgaacgtggagctcacttccat
tctttaaccgatagtattgataccagtagcgcgatggggcgattcttttttcatgtaatgtcagcactggccgagatgg
agcgagaattaatcgtcgagcgaacccttgccggactggctgccgccagagcgcaaggacgactgggagggcgccctcg
ggcgatcaacaaacatgaacaggaacagattagtcggctattagagaaaggccatcctcggcagcaattagctattatt
tttggtattggcgtatccaccttatacagatactttccggcaagcagtataaaaaaacgaatgaattaatactagagaa
aaaaaaaccccgcccctgacagggcggggtttttttt