LOCUS       BBa_K318013             1400 bp    DNA     linear       17-May-2024
DEFINITION  T + pLac + RBS + Int (P22) + T
ACCESSION   BBa_K318013
VERSION     BBa_K318013.1  
FEATURES             Location/Qualifiers
     misc_feature    1..39
                     /label=B1006
     stem_loop       10..31
                     /label=modified thr terminator
     polyA_site      32..39
                     /label=PolyA
     polyA_site      1..9
                     /label=PolyA
     terminator      1..39
                     /label=BBa_B1006
     misc_feature    50..66
                     /label=lac O1
     promoter        67..72
                     /label=-35
     misc_feature    73..89
                     /label=lac O1
     promoter        90..95
                     /label=-10
     misc_feature    48..101
                     /label=BBa_R0011
     promoter        48..101
                     /label=BBa_R0011
     misc_feature    115..118
                     /label=conserved
     RBS             111..122
                     /label=BBa_B0034
     misc_feature    48..122
                     /label=BBa_K200021
     CDS             129..1289
                     /label=Integrase p22
     misc_feature    1290..1322
                     /label=LVA
     misc_feature    1323..1328
                     /label=stop
     misc_feature    1329..1353
                     /label=Help:Barcodes
     CDS             129..1353
                     /label=BBa_I11030
     misc_feature    1362..1400
                     /label=B1006
     stem_loop       1371..1392
                     /label=modified thr terminator
     polyA_site      1393..1400
                     /label=PolyA
     polyA_site      1362..1370
                     /label=PolyA
     terminator      1362..1400
                     /label=BBa_B1006
ORIGIN
        1 aaaaaaaaac cccgcccctg acagggcggg gttttttttt actagagaat tgtgagcgga
       61 taacaattga cattgtgagc ggataacaag atactgagca catactagag aaagaggaga
      121 aatactagat gtcactattc cgcagaggtg aaacctggta cgccagtttc acattgccga
      181 acggcaaaag atttaagcag tctcttggga caaaggacaa aaggcaggcc acagagcttc
      241 atgacaagct gaaggcagaa gcatggaggg taagtaaatt aggagagacg cctgacatga
      301 cttttgaggg ggcctgtgtc aggtggttag aggagaaggc gcataagaag tcgctggatg
      361 atgacaagag tcggatagga ttctggctcc agcattttgc aggaatgcag ttgaaggata
      421 ttaccgagac gaagatttac tccgccatcc agaagataac taatcggcgg catgaggaaa
      481 actggaagtt aatggatgaa gcatgcagga agaatgggaa gcagcctccg gtattcaagc
      541 ctaagccggc agcagtagct acaaaagcaa ctcacctttc attcattaag gcactcctcc
      601 gggctgctga acgcgaatgg aagatgctgg ataaggctcc gatcatcaaa gttcctcagc
      661 cgaaaaataa gcgtatccgc tggcttgagc ctcacgaggc aaaaaggttg attgatgaat
      721 gccaggaacc gctaaagtca gtcgtagagt ttgcgctttc tactggctta aggcggtcta
      781 acattatcaa tctggagtgg cagcagatag acatgcaacg aaaggtggca tggatacacc
      841 cggaacaaag caagtctaat catgccattg gagtggcgct gaatgatacc gcttgccggg
      901 tgctgaaaaa gcaaatcggc aatcatcaca aatgggtgtt cgtctacaag gaaagcagca
      961 ccaaaccaga cggaactaaa tcacctgtag tgaggaagat gcgctatgac gctaatactg
     1021 catggagggc agcattaaaa cgagcgggca ttgaagactt ccgttttcat gacctgaggc
     1081 acacgtgggc aagttggtta gttcaggctg gcgttccgat ttcggtattg caggaaatgg
     1141 gtggctggga gtctatcgaa atggttcgcc gatatgctca tctggcacca aatcacctga
     1201 ctgaacatgc tcgacaaatt gactcgattt ttggtacttc tgtcccaaat atgtcccaca
     1261 gtaaaaataa ggaaggcacg aataatacgg cagcaaacga cgaaaactac gctttagtag
     1321 cttaataacc ctgatagtgc tagtgtagat ccctactaga gaaaaaaaaa ccccgcccct
     1381 gacagggcgg ggtttttttt
//