LOCUS       BBa_K318014             1931 bp    DNA     linear       25-Apr-2024
DEFINITION  pCons + attB + RBS + GFP + T + rRFP + rRBS + attP
ACCESSION   BBa_K318014
VERSION     BBa_K318014.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    44..70
                     /label=BBa_I11032
     RBS             79..91
                     /label=RBS-3\Weak
     RBS             85..88
                     /label=RBS
     misc_feature    79..91
                     /label=BBa_B0032
     RBS             79..91
                     /label=BBa_B0032
     CDS             98..817
                     /label=GFP protein
     CDS             98..817
                     /label=BBa_E0040
     stem_loop       837..880
                     /label=stem_loop
     misc_feature    826..905
                     /label=BBa_B0010
     terminator      826..905
                     /label=BBa_B0010
     stem_loop       921..940
                     /label=T7 TE
     misc_feature    947..947
                     /label=stop
     polyA_site      941..954
                     /label=polya
     misc_feature    914..954
                     /label=BBa_B0012
     terminator      914..954
                     /label=BBa_B0012
     CDS             963..1640
                     /label=mRFP reverse
     primer_bind     1621..1640
                     /label=Spe mRFP F
     primer_bind     963..982
                     /label=Xba mRFP R
     misc_feature    963..1640
                     /label=BBa_J31008
     RBS             1649..1663
                     /label=Reverse RBS
     RBS             1649..1663
                     /label=BBa_J44001
     misc_feature    1672..1931
                     /label=BBa_I11033
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagacgacct tcgcattacg
       61 aatgcgctgc tactagagtc acacaggaaa gtactagatg cgtaaaggag aagaactttt
      121 cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc
      181 tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg
      241 cactactgga aaactacctg ttccatggcc aacacttgtc actactttcg gttatggtgt
      301 tcaatgcttt gcgagatacc cagatcatat gaaacagcat gactttttca agagtgccat
      361 gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac
      421 acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat
      481 tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca
      541 caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag
      601 acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat
      661 tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc
      721 gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg
      781 gattacacat ggcatggatg aactatacaa ataataatac tagagccagg catcaaataa
      841 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
      901 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag
      961 agttaagcac cggtggagtg acgaccttca gcacgttcgt actgttcaac gatggtgtag
     1021 tcttcgttgt gggaggtgat gtccagtttg atgtcggttt tgtaagcacc cggcagctga
     1081 accggttttt tagccatgta ggtggtttta acttcagcgt cgtagtgacc accgtctttc
     1141 agtttcagac gcattttgat ttcacctttc agagcaccgt cttccgggta catacgttcg
     1201 gtggaagctt cccaacccat ggtttttttc tgcataaccg gaccgtcgga cgggaagttg
     1261 gtaccacgca gtttaacttt gtagatgaac tcaccgtctt gcagggagga gtcctgggta
     1321 acggtaacaa caccaccgtc ttcgaagttc ataacacgtt cccatttgaa accttccggg
     1381 aaggacagtt tcaggtagtc cgggatgtca gccgggtgtt taacgtaagc tttggaaccg
     1441 tactggaact gcggggacag gatgtcccaa gcgaacggca gcggaccacc tttggtaact
     1501 ttcagtttag cggtctgggt accttcgtac ggacgacctt caccttcacc ttcgatttcg
     1561 aactcgtgac cgttaacgga accttccata cgaactttga aacgcatgaa ctctttgata
     1621 acgtcttcgg aggaagccat tactagagtt tctcctcttt aattactaga gctaagtggt
     1681 ttgggacaaa aatgggacat acaaatcttt gcatcggttt gcaaggcttt gcatgtcttt
     1741 cgaagatggg acgtgtgagc gcaggtatga cgtggtatgt tgttgactta aaaggtagtt
     1801 cttataattc gtaatgcgaa ggtcgtaggt tcgactccta ttatcggcac cagttaaatc
     1861 aaatacttac gtattattcg tgccttcctt atttttactg tgggacatat ttgggacaga
     1921 agtaccaaaa a
//