BBa_K318014
type: ComponentDefinition
persistent identity: BBa K318014
display id: BBa_K318014
version: 1
was generated by: iGEM to SBOL conversion
description: pCons + attB + RBS + GFP + T + rRFP + rRBS + attP
created: 2010-07-12T11:00:00Z
modified: 2015-05-08T01:11:57Z
mutable provenance: Biobricks
owned by: james
owned by: myers
mutable description: This composite part is a testing cassette for a Cre-like, P22 Int recombination. The constitutive promoter should express GFP if recombination does not occur. If Cre-like recombination occurs, this part should express RFP. It is composed of the following biobricks: BBa_J23100, BBa_I11032, BBa_E0240, BBa_K199021, BBa_I11033.
discontinued: false
dominant: false
experience: experience/None
group u list: _438_
m user id: 0
owner id: 4845
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The length of DNA between attB and attP might not be sufficient for recombination.
star: false
creator: Nathaniel Pantalone, Justin Vrana
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_J44001
component: BBa_J31008
component: BBa_I11032
component: BBa_J23100
component: BBa_E0040
component: BBa_B0032
component: BBa_I11033
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_J23100
sequence annotation: BBa_I11032
sequence annotation: BBa_B0032
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_J31008
sequence annotation: BBa_J44001
sequence annotation: BBa_I11033
sequence: https://synbiohub.org/public/igem/BBa_K318014_sequence/1

BBa_J44001
type: Component
persistent identity: component2073439
display id: component2073439
version: 1
top level: BBa_K318014
definition: BBa_J44001
access: public

BBa_J31008
type: Component
persistent identity: component2073437
display id: component2073437
version: 1
top level: BBa_K318014
definition: mRFPr
access: public

BBa_I11032
type: Component
persistent identity: component2073422
display id: component2073422
version: 1
top level: BBa_K318014
definition: P22 attB
access: public

BBa_J23100
type: Component
persistent identity: component2073421
display id: component2073421
version: 1
top level: BBa_K318014
definition: BBa_J23100
access: public

BBa_E0040
type: Component
persistent identity: component2073427
display id: component2073427
version: 1
top level: BBa_K318014
definition: GFP
access: public

BBa_B0032
type: Component
persistent identity: component2073424
display id: component2073424
version: 1
top level: BBa_K318014
definition: BBa_B0032
access: public

BBa_I11033
type: Component
persistent identity: component2073440
display id: component2073440
version: 1
top level: BBa_K318014
definition: P22 attP
access: public

BBa_B0012
type: Component
persistent identity: component2073430
display id: component2073430
version: 1
top level: BBa_K318014
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2073428
display id: component2073428
version: 1
top level: BBa_K318014
definition: BBa_B0010
access: public

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2073421
display id: annotation2073421
version: 1
top level: BBa_K318014
location: https://synbiohub.org/public/igem/BBa_K318014/annotation2073421/range2073421/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K318014/annotation2073421/range2073421/1
type: Range
persistent identity: range2073421
display id: range2073421
version: 1
top level: BBa_K318014
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_I11032
type: SequenceAnnotation
persistent identity: annotation2073422
display id: annotation2073422
version: 1
top level: BBa_K318014
location: https://synbiohub.org/public/igem/BBa_K318014/annotation2073422/range2073422/1
component: BBa_I11032

https://synbiohub.org/public/igem/BBa_K318014/annotation2073422/range2073422/1
type: Range
persistent identity: range2073422
display id: range2073422
version: 1
top level: BBa_K318014
direction: direction/reverse
start: 44
end: 70
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2073424
display id: annotation2073424
version: 1
top level: BBa_K318014
location: https://synbiohub.org/public/igem/BBa_K318014/annotation2073424/range2073424/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K318014/annotation2073424/range2073424/1
type: Range
persistent identity: range2073424
display id: range2073424
version: 1
top level: BBa_K318014
direction: direction/forward
start: 79
end: 91
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2073427
display id: annotation2073427
version: 1
top level: BBa_K318014
location: https://synbiohub.org/public/igem/BBa_K318014/annotation2073427/range2073427/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K318014/annotation2073427/range2073427/1
type: Range
persistent identity: range2073427
display id: range2073427
version: 1
top level: BBa_K318014
direction: direction/reverse
start: 98
end: 817
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2073428
display id: annotation2073428
version: 1
top level: BBa_K318014
location: https://synbiohub.org/public/igem/BBa_K318014/annotation2073428/range2073428/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K318014/annotation2073428/range2073428/1
type: Range
persistent identity: range2073428
display id: range2073428
version: 1
top level: BBa_K318014
direction: direction/forward
start: 826
end: 905
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2073430
display id: annotation2073430
version: 1
top level: BBa_K318014
location: https://synbiohub.org/public/igem/BBa_K318014/annotation2073430/range2073430/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K318014/annotation2073430/range2073430/1
type: Range
persistent identity: range2073430
display id: range2073430
version: 1
top level: BBa_K318014
direction: direction/forward
start: 914
end: 954
orientation: inline

BBa_J31008
type: SequenceAnnotation
persistent identity: annotation2073437
display id: annotation2073437
version: 1
top level: BBa_K318014
location: https://synbiohub.org/public/igem/BBa_K318014/annotation2073437/range2073437/1
component: BBa_J31008

https://synbiohub.org/public/igem/BBa_K318014/annotation2073437/range2073437/1
type: Range
persistent identity: range2073437
display id: range2073437
version: 1
top level: BBa_K318014
direction: direction/reverse
start: 963
end: 1640
orientation: inline

BBa_J44001
type: SequenceAnnotation
persistent identity: annotation2073439
display id: annotation2073439
version: 1
top level: BBa_K318014
location: https://synbiohub.org/public/igem/BBa_K318014/annotation2073439/range2073439/1
component: BBa_J44001

https://synbiohub.org/public/igem/BBa_K318014/annotation2073439/range2073439/1
type: Range
persistent identity: range2073439
display id: range2073439
version: 1
top level: BBa_K318014
direction: direction/reverse
start: 1649
end: 1663
orientation: inline

BBa_I11033
type: SequenceAnnotation
persistent identity: annotation2073440
display id: annotation2073440
version: 1
top level: BBa_K318014
location: https://synbiohub.org/public/igem/BBa_K318014/annotation2073440/range2073440/1
component: BBa_I11033

https://synbiohub.org/public/igem/BBa_K318014/annotation2073440/range2073440/1
type: Range
persistent identity: range2073440
display id: range2073440
version: 1
top level: BBa_K318014
direction: direction/reverse
start: 1672
end: 1931
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

BBa_J44001
type: ComponentDefinition
persistent identity: BBa J44001
display id: BBa_J44001
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse RBS (RBSrev) -- corresponds to BBa_B0030
created: 2006-08-01T11:00:00Z
modified: 2015-08-31T04:08:48Z
mutable provenance: Cloned from synthetic oligos
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Reverse version of RBS BBa_B0030
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_71_
m user id: 0
owner id: 606
owning group id: 61
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Repeats in oligos caused unusual products during cloning
star: true
creator: Todd Eckdahl
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: Reverse RBS
sequence: https://synbiohub.org/public/igem/BBa_J44001_sequence/1

Reverse RBS
type: SequenceAnnotation
persistent identity: annotation1893199
display id: annotation1893199
version: 1
top level: BBa_J44001
location: https://synbiohub.org/public/igem/BBa_J44001/annotation1893199/range1893199/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_J44001/annotation1893199/range1893199/1
type: Range
persistent identity: range1893199
display id: range1893199
version: 1
top level: BBa_J44001
direction: direction/reverse
start: 1
end: 15
orientation: inline

mRFPr
type: ComponentDefinition
persistent identity: BBa J31008
display id: BBa_J31008
version: 1
was generated by: iGEM to SBOL conversion
description: RFP reverse
created: 2007-02-15T12:00:00Z
modified: 2015-08-31T04:08:45Z
mutable provenance: RFP reverse was PCR amplified from mRFP (BBaE1010).
owned by: james
owned by: myers
mutable description: The mRFP coding sequence in the reverse orientation. This part requires a reverse RBS and reverse promoter to be expressed
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 1144
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: RFP reverse was PCR amplified from mRFP (BBaE1010) using the following primers: 5'-atgcactagtatggcttcctccgaagacgt "Spe mRFP F" 5'-gcattctagattaagcaccggtggagtgac "Xba mRFP R"
star: false
creator: Karmella Haynes
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: mRFP reverse
sequence annotation: Xba mRFP R
sequence annotation: Spe mRFP F
sequence: https://synbiohub.org/public/igem/BBa_J31008_sequence/1

mRFP reverse
type: SequenceAnnotation
persistent identity: annotation1911761
display id: annotation1911761
version: 1
top level: mRFPr
location: https://synbiohub.org/public/igem/BBa_J31008/annotation1911761/range1911761/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J31008/annotation1911761/range1911761/1
type: Range
persistent identity: range1911761
display id: range1911761
version: 1
top level: mRFPr
direction: direction/reverse
start: 1
end: 678
orientation: inline

Xba mRFP R
type: SequenceAnnotation
persistent identity: annotation1911765
display id: annotation1911765
version: 1
top level: mRFPr
location: https://synbiohub.org/public/igem/BBa_J31008/annotation1911765/range1911765/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/BBa_J31008/annotation1911765/range1911765/1
type: Range
persistent identity: range1911765
display id: range1911765
version: 1
top level: mRFPr
direction: direction/forward
start: 1
end: 20
orientation: inline

Spe mRFP F
type: SequenceAnnotation
persistent identity: annotation1911764
display id: annotation1911764
version: 1
top level: mRFPr
location: https://synbiohub.org/public/igem/BBa_J31008/annotation1911764/range1911764/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/BBa_J31008/annotation1911764/range1911764/1
type: Range
persistent identity: range1911764
display id: range1911764
version: 1
top level: mRFPr
direction: direction/reverse
start: 659
end: 678
orientation: inline

P22 attB
type: ComponentDefinition
persistent identity: BBa I11032
display id: BBa_I11032
version: 1
was generated by: iGEM to SBOL conversion
description: P22 ''attB'', reverse complement
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:30Z
mutable provenance: gi|49175990:262118-262144 rev comp
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Reverse Complement of P22 attB site (B'-O-B)
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: mschomp
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_I11032_sequence/1

P22 attP
type: ComponentDefinition
persistent identity: BBa I11033
display id: BBa_I11033
version: 1
was generated by: iGEM to SBOL conversion
description: P22 ''attP''
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:30Z
mutable provenance: gi|51236723:21158-21417
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: P22 phage attP site (P-O-P')
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: 2004 Boston University iGEM team
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_I11033_sequence/1

https://synbiohub.org/public/igem/BBa_K318014_sequence/1
type: Sequence
persistent identity: BBa K318014 sequence
display id: BBa_K318014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagacgaccttcgcattacgaatgcgctgctactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatgtccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgtcgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttcggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaactttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacacgttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttggaaccgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtctgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaactttgaaacgcatgaactctttgataacgtcttcggaggaagccattactagagtttctcctctttaattactagagctaagtggtttgggacaaaaatgggacatacaaatctttgcatcggtttgcaaggctttgcatgtctttcgaagatgggacgtgtgagcgcaggtatgacgtggtatgttgttgacttaaaaggtagttcttataattcgtaatgcgaaggtcgtaggttcgactcctattatcggcaccagttaaatcaaatacttacgtattattcgtgccttccttatttttactgtgggacatatttgggacagaagtaccaaaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23100_sequence/1
type: Sequence
persistent identity: BBa J23100 sequence
display id: BBa_J23100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J44001_sequence/1
type: Sequence
persistent identity: BBa J44001 sequence
display id: BBa_J44001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttctcctctttaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J31008_sequence/1
type: Sequence
persistent identity: BBa J31008 sequence
display id: BBa_J31008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatgtccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgtcgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttcggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaactttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacacgttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttggaaccgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtctgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaactttgaaacgcatgaactctttgataacgtcttcggaggaagccat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I11032_sequence/1
type: Sequence
persistent identity: BBa I11032 sequence
display id: BBa_I11032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acgaccttcgcattacgaatgcgctgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I11033_sequence/1
type: Sequence
persistent identity: BBa I11033 sequence
display id: BBa_I11033_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctaagtggtttgggacaaaaatgggacatacaaatctttgcatcggtttgcaaggctttgcatgtctttcgaagatgggacgtgtgagcgcaggtatgacgtggtatgttgttgacttaaaaggtagttcttataattcgtaatgcgaaggtcgtaggttcgactcctattatcggcaccagttaaatcaaatacttacgtattattcgtgccttccttatttttactgtgggacatatttgggacagaagtaccaaaaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z