LOCUS       BBa_K318015             2017 bp    DNA     linear       17-May-2024
DEFINITION  Hin Generator (T + pLac + RBS + Hin + T + pCons + RBS + lacIq + T)
ACCESSION   BBa_K318015
VERSION     BBa_K318015.1  
FEATURES             Location/Qualifiers
     misc_feature    1..39
                     /label=B1006
     stem_loop       10..31
                     /label=modified thr terminator
     polyA_site      32..39
                     /label=PolyA
     polyA_site      1..9
                     /label=PolyA
     terminator      1..39
                     /label=BBa_B1006
     misc_feature    50..66
                     /label=lac O1
     promoter        67..72
                     /label=-35
     misc_feature    73..89
                     /label=lac O1
     promoter        90..95
                     /label=-10
     misc_feature    48..101
                     /label=BBa_R0011
     promoter        48..101
                     /label=BBa_R0011
     misc_feature    115..118
                     /label=conserved
     RBS             111..122
                     /label=BBa_B0034
     misc_feature    48..122
                     /label=BBa_K200021
     misc_feature    198..203
                     /label=Former SpeI site
     misc_feature    200..200
                     /label=Mutation to delete the SpeI site
     CDS             129..701
                     /label=Hin sequence
     primer_bind     129..131
                     /label=Fwd primer J31001
     primer_bind     679..698
                     /label=Rev primer J31001
     CDS             129..701
                     /label=BBa_J31000
     misc_feature    710..748
                     /label=B1006
     stem_loop       719..740
                     /label=modified thr terminator
     polyA_site      741..748
                     /label=PolyA
     polyA_site      710..718
                     /label=PolyA
     terminator      710..748
                     /label=BBa_B1006
     promoter        757..791
                     /label=BBa_J23100
     misc_feature    804..807
                     /label=conserved
     RBS             800..811
                     /label=BBa_B0034
     misc_feature    1907..1945
                     /label=LVA
     CDS             818..1945
                     /label=lacI-LVA
     misc_feature    818..1945
                     /label=BBa_C0012
     misc_feature    1946..1970
                     /label=Help:Barcodes
     CDS             818..1945
                     /label=BBa_C0012
     misc_feature    800..1970
                     /label=BBa_S03520
     misc_feature    1979..2017
                     /label=B1006
     stem_loop       1988..2009
                     /label=modified thr terminator
     polyA_site      2010..2017
                     /label=PolyA
     polyA_site      1979..1987
                     /label=PolyA
     terminator      1979..2017
                     /label=BBa_B1006
ORIGIN
        1 aaaaaaaaac cccgcccctg acagggcggg gttttttttt actagagaat tgtgagcgga
       61 taacaattga cattgtgagc ggataacaag atactgagca catactagag aaagaggaga
      121 aatactagat ggctactatt gggtatattc gggtgtcaac aattgaccaa aatatcgatt
      181 tacagcgtaa tgcgcttacc agtgcaaatt gtgaccgcat ttttgaagac cgtatcagtg
      241 gcaagattgc aaaccgcccc ggcctgaaac gggcgttaaa gtatgtaaat aaaggcgata
      301 ctcttgtcgt ctggaaatta gacagactgg gccgtagcgt gaaaaatctg gtggcgttaa
      361 tatcagaatt acatgaacgt ggagctcact tccattcttt aaccgatagt attgatacca
      421 gtagcgcgat ggggcgattc ttttttcatg taatgtcagc actggccgag atggagcgag
      481 aattaatcgt cgagcgaacc cttgccggac tggctgccgc cagagcgcaa ggacgactgg
      541 gagggcgccc tcgggcgatc aacaaacatg aacaggaaca gattagtcgg ctattagaga
      601 aaggccatcc tcggcagcaa ttagctatta tttttggtat tggcgtatcc accttataca
      661 gatactttcc ggcaagcagt ataaaaaaac gaatgaatta atactagaga aaaaaaaacc
      721 ccgcccctga cagggcgggg ttttttttta ctagagttga cggctagctc agtcctaggt
      781 acagtgctag ctactagaga aagaggagaa atactagatg gtgaatgtga aaccagtaac
      841 gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa
      901 ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct
      961 gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg
     1021 cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc
     1081 tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga
     1141 agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa
     1201 ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc
     1261 gttatttctt gatgtctctg accagacacc catcaacagt attattttct cccatgaaga
     1321 cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt
     1381 agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct
     1441 cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg
     1501 ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc
     1561 caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg
     1621 tgcggatatc tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc
     1681 gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct
     1741 gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa
     1801 aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     1861 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcaggctg caaacgacga
     1921 aaactacgct ttagtagctt aataactctg atagtgctag tgtagatctc tactagagaa
     1981 aaaaaaaccc cgcccctgac agggcggggt ttttttt
//