##gff-version 3 ##sequence-region BBa_K318015 1 2017 BBa_K318015 . terminator 1 39 . + 0 ID=BBa_B1006;Name=BBa_B1006 BBa_K318015 . polyA_site 1 9 . + 0 ID=annotation1898431;Name=PolyA;Parent=BBa_B1006 BBa_K318015 . engineered_region 1 39 . + 0 ID=annotation1898428;Name=B1006;Parent=BBa_B1006 BBa_K318015 . polyA_site 32 39 . + 0 ID=annotation1898430;Name=PolyA;Parent=BBa_B1006 BBa_K318015 . stem_loop 10 31 . + 0 ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006 BBa_K318015 . CDS 129 701 . + 0 ID=BBa_J31000;Name=BBa_J31000 BBa_K318015 . sequence_alteration 200 200 . + 0 ID=annotation1880460;Name=Mutation to delete the SpeI site;Parent=BBa_J31000 BBa_K318015 . primer_binding_site 129 131 . + 0 ID=annotation1910568;Name=Fwd primer J31001;Parent=BBa_J31000 BBa_K318015 . sequence_alteration 198 203 . + 0 ID=annotation1880459;Name=Former SpeI site;Parent=BBa_J31000 BBa_K318015 . primer_binding_site 679 698 . + 0 ID=annotation1910569;Name=Rev primer J31001;Parent=BBa_J31000 BBa_K318015 . CDS 129 701 . + 0 ID=annotation1884987;Name=Hin sequence;Parent=BBa_J31000 BBa_K318015 . engineered_region 800 1970 . + 0 ID=BBa_S03520;Name=BBa_S03520 BBa_K318015 . ribosome_entry_site 800 811 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03520 BBa_K318015 . conserved_region 804 807 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_K318015 . CDS 818 1945 . + 0 ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_S03520 BBa_K318015 . engineered_region 818 1945 . + 0 ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012 BBa_K318015 . tag 1907 1945 . + 0 ID=annotation1722;Name=LVA;Parent=BBa_C0012 BBa_K318015 . engineered_tag 1946 1970 . + 0 ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012 BBa_K318015 . CDS 818 1945 . + 0 ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012 BBa_K318015 . engineered_region 48 122 . + 0 ID=BBa_K200021;Name=BBa_K200021 BBa_K318015 . promoter 48 101 . + 0 ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_K200021 BBa_K318015 . promoter 67 72 . + 0 ID=annotation2000;Name=-35;Parent=BBa_R0011 BBa_K318015 . promoter 90 95 . + 0 ID=annotation2002;Name=-10;Parent=BBa_R0011 BBa_K318015 . operator 73 89 . + 0 ID=annotation2001;Name=lac O1;Parent=BBa_R0011 BBa_K318015 . engineered_region 48 101 . + 0 ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011 BBa_K318015 . operator 50 66 . + 0 ID=annotation1999;Name=lac O1;Parent=BBa_R0011 BBa_K318015 . ribosome_entry_site 111 122 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K200021 BBa_K318015 . conserved_region 115 118 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_K318015 . terminator 1979 2017 . + 0 ID=BBa_B1006;Name=BBa_B1006 BBa_K318015 . polyA_site 1979 1987 . + 0 ID=annotation1898431;Name=PolyA;Parent=BBa_B1006 BBa_K318015 . engineered_region 1979 2017 . + 0 ID=annotation1898428;Name=B1006;Parent=BBa_B1006 BBa_K318015 . polyA_site 2010 2017 . + 0 ID=annotation1898430;Name=PolyA;Parent=BBa_B1006 BBa_K318015 . stem_loop 1988 2009 . + 0 ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006 BBa_K318015 . promoter 757 791 . + 0 ID=BBa_J23100;Name=BBa_J23100 BBa_K318015 . terminator 710 748 . + 0 ID=BBa_B1006;Name=BBa_B1006 BBa_K318015 . polyA_site 710 718 . + 0 ID=annotation1898431;Name=PolyA;Parent=BBa_B1006 BBa_K318015 . engineered_region 710 748 . + 0 ID=annotation1898428;Name=B1006;Parent=BBa_B1006 BBa_K318015 . polyA_site 741 748 . + 0 ID=annotation1898430;Name=PolyA;Parent=BBa_B1006 BBa_K318015 . stem_loop 719 740 . + 0 ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006 >BBa_K318015 aaaaaaaaaccccgcccctgacagggcggggtttttttttactagagaattgtgagcggataacaattgacattgtgag cggataacaagatactgagcacatactagagaaagaggagaaatactagatggctactattgggtatattcgggtgtca acaattgaccaaaatatcgatttacagcgtaatgcgcttaccagtgcaaattgtgaccgcatttttgaagaccgtatca gtggcaagattgcaaaccgccccggcctgaaacgggcgttaaagtatgtaaataaaggcgatactcttgtcgtctggaa attagacagactgggccgtagcgtgaaaaatctggtggcgttaatatcagaattacatgaacgtggagctcacttccat tctttaaccgatagtattgataccagtagcgcgatggggcgattcttttttcatgtaatgtcagcactggccgagatgg agcgagaattaatcgtcgagcgaacccttgccggactggctgccgccagagcgcaaggacgactgggagggcgccctcg ggcgatcaacaaacatgaacaggaacagattagtcggctattagagaaaggccatcctcggcagcaattagctattatt tttggtattggcgtatccaccttatacagatactttccggcaagcagtataaaaaaacgaatgaattaatactagagaa aaaaaaaccccgcccctgacagggcggggtttttttttactagagttgacggctagctcagtcctaggtacagtgctag ctactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtg tctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggc gatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgcc acctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcg tggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcag tgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgtta tttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagc atctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggc tggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggt tttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgg gcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaaga cagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttg ctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctgg cgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactgga aagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctacta gagaaaaaaaaaccccgcccctgacagggcggggtttttttt