BBa_K318015
type: ComponentDefinition
persistent identity: BBa K318015
display id: BBa_K318015
version: 1
was generated by: iGEM to SBOL conversion
description: Hin Generator (T + pLac + RBS + Hin + T + pCons + RBS + lacIq + T)
created: 2010-07-12T11:00:00Z
modified: 2015-05-08T01:11:57Z
mutable provenance: Assembled from other biobrick parts.
owned by: james
owned by: myers
mutable description: A Hin Generator is a clone composed of the parts: BBa_B1006+BBa_K200021+BBa_J31000+BBa_B1006+BBa_J23100+BBa_S03520+BBa_B1006. It produces the Hin Recombinase enzyme (BBa_J31000) in response to IPTG.
discontinued: false
dominant: false
experience: experience/None
group u list: _438_
m user id: 0
owner id: 6061
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Constitutive promoter was placed after the hin recombinase to prevent unwanted Hin Recombinase
star: false
creator: Justin Vrana, Nate Pantalone
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B1006
component: BBa_J31000
component: BBa_K200021
component: BBa_J23100
component: BBa_S03520
sequence annotation: BBa_B1006
sequence annotation: BBa_K200021
sequence annotation: BBa_J31000
sequence annotation: BBa_J23100
sequence annotation: BBa_S03520
sequence: https://synbiohub.org/public/igem/BBa_K318015_sequence/1

BBa_B1006
type: Component
persistent identity: component2277307
display id: component2277307
version: 1
top level: BBa_K318015
definition: BBa_B1006
access: public

BBa_J31000
type: Component
persistent identity: component2277321
display id: component2277321
version: 1
top level: BBa_K318015
definition: Hin
access: public

BBa_K200021
type: Component
persistent identity: component2277315
display id: component2277315
version: 1
top level: BBa_K318015
definition: BBa_K200021
access: public

BBa_J23100
type: Component
persistent identity: component2277327
display id: component2277327
version: 1
top level: BBa_K318015
definition: BBa_J23100
access: public

BBa_B1006
type: Component
persistent identity: component2277326
display id: component2277326
version: 1
top level: BBa_K318015
definition: BBa_B1006
access: public

BBa_B1006
type: Component
persistent identity: component2277339
display id: component2277339
version: 1
top level: BBa_K318015
definition: BBa_B1006
access: public

BBa_S03520
type: Component
persistent identity: component2277334
display id: component2277334
version: 1
top level: BBa_K318015
definition: BBa_S03520
access: public

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2277307
display id: annotation2277307
version: 1
top level: BBa_K318015
location: https://synbiohub.org/public/igem/BBa_K318015/annotation2277307/range2277307/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K318015/annotation2277307/range2277307/1
type: Range
persistent identity: range2277307
display id: range2277307
version: 1
top level: BBa_K318015
direction: direction/reverse
start: 1
end: 39
orientation: inline

BBa_K200021
type: SequenceAnnotation
persistent identity: annotation2277315
display id: annotation2277315
version: 1
top level: BBa_K318015
location: https://synbiohub.org/public/igem/BBa_K318015/annotation2277315/range2277315/1
component: BBa_K200021

https://synbiohub.org/public/igem/BBa_K318015/annotation2277315/range2277315/1
type: Range
persistent identity: range2277315
display id: range2277315
version: 1
top level: BBa_K318015
direction: direction/reverse
start: 48
end: 122
orientation: inline

BBa_J31000
type: SequenceAnnotation
persistent identity: annotation2277321
display id: annotation2277321
version: 1
top level: BBa_K318015
location: https://synbiohub.org/public/igem/BBa_K318015/annotation2277321/range2277321/1
component: BBa_J31000

https://synbiohub.org/public/igem/BBa_K318015/annotation2277321/range2277321/1
type: Range
persistent identity: range2277321
display id: range2277321
version: 1
top level: BBa_K318015
direction: direction/reverse
start: 129
end: 701
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2277326
display id: annotation2277326
version: 1
top level: BBa_K318015
location: https://synbiohub.org/public/igem/BBa_K318015/annotation2277326/range2277326/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K318015/annotation2277326/range2277326/1
type: Range
persistent identity: range2277326
display id: range2277326
version: 1
top level: BBa_K318015
direction: direction/reverse
start: 710
end: 748
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2277327
display id: annotation2277327
version: 1
top level: BBa_K318015
location: https://synbiohub.org/public/igem/BBa_K318015/annotation2277327/range2277327/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K318015/annotation2277327/range2277327/1
type: Range
persistent identity: range2277327
display id: range2277327
version: 1
top level: BBa_K318015
direction: direction/reverse
start: 757
end: 791
orientation: inline

BBa_S03520
type: SequenceAnnotation
persistent identity: annotation2277334
display id: annotation2277334
version: 1
top level: BBa_K318015
location: https://synbiohub.org/public/igem/BBa_K318015/annotation2277334/range2277334/1
component: BBa_S03520

https://synbiohub.org/public/igem/BBa_K318015/annotation2277334/range2277334/1
type: Range
persistent identity: range2277334
display id: range2277334
version: 1
top level: BBa_K318015
direction: direction/reverse
start: 800
end: 1970
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2277339
display id: annotation2277339
version: 1
top level: BBa_K318015
location: https://synbiohub.org/public/igem/BBa_K318015/annotation2277339/range2277339/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K318015/annotation2277339/range2277339/1
type: Range
persistent identity: range2277339
display id: range2277339
version: 1
top level: BBa_K318015
direction: direction/reverse
start: 1979
end: 2017
orientation: inline

BBa_S03520
type: ComponentDefinition
persistent identity: BBa S03520
display id: BBa_S03520
version: 1
was generated by: iGEM to SBOL conversion
description: B0034:C0012
created: 2006-07-23T11:00:00Z
modified: 2015-05-08T01:14:24Z
owned by: james
owned by: myers
part status: Released HQ 2013
discontinued: false
dominant: false
experience: experience/None
group u list: _58_
m user id: 0
owner id: 1061
owning group id: 58
sample status: In stock
status: status/Available
bookmark: true
star: true
creator: Azeem Kaka
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_C0012
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0012
sequence: https://synbiohub.org/public/igem/BBa_S03520_sequence/1

BBa_C0012
type: Component
persistent identity: component2243695
display id: component2243695
version: 1
top level: BBa_S03520
definition: lacI
access: public

BBa_B0034
type: Component
persistent identity: component2243693
display id: component2243693
version: 1
top level: BBa_S03520
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2243693
display id: annotation2243693
version: 1
top level: BBa_S03520
location: https://synbiohub.org/public/igem/BBa_S03520/annotation2243693/range2243693/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S03520/annotation2243693/range2243693/1
type: Range
persistent identity: range2243693
display id: range2243693
version: 1
top level: BBa_S03520
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2243695
display id: annotation2243695
version: 1
top level: BBa_S03520
location: https://synbiohub.org/public/igem/BBa_S03520/annotation2243695/range2243695/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_S03520/annotation2243695/range2243695/1
type: Range
persistent identity: range2243695
display id: range2243695
version: 1
top level: BBa_S03520
direction: direction/forward
start: 19
end: 1146
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

BBa_B1006
type: ComponentDefinition
persistent identity: BBa B1006
display id: BBa_B1006
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (artificial, large, %T~>90)
created: 2006-08-30T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90%
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues.
star: true
creator: Haiyao Huang
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: B1006
sequence annotation: PolyA
sequence annotation: modified thr terminator
sequence: https://synbiohub.org/public/igem/BBa_B1006_sequence/1

B1006
type: SequenceAnnotation
persistent identity: annotation1898428
display id: annotation1898428
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
type: Range
persistent identity: range1898428
display id: range1898428
version: 1
top level: BBa_B1006
direction: direction/forward
start: 1
end: 39
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898431
display id: annotation1898431
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
type: Range
persistent identity: range1898431
display id: range1898431
version: 1
top level: BBa_B1006
direction: direction/reverse
start: 1
end: 9
orientation: inline

modified thr terminator
type: SequenceAnnotation
persistent identity: annotation1898429
display id: annotation1898429
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
type: Range
persistent identity: range1898429
display id: range1898429
version: 1
top level: BBa_B1006
direction: direction/forward
start: 10
end: 31
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898430
display id: annotation1898430
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
type: Range
persistent identity: range1898430
display id: range1898430
version: 1
top level: BBa_B1006
direction: direction/forward
start: 32
end: 39
orientation: inline

Hin
type: ComponentDefinition
persistent identity: BBa J31000
display id: BBa_J31000
version: 1
was generated by: iGEM to SBOL conversion
description: DNA-invertase Hin from Salmonella typhimurium
created: 2006-06-04T11:00:00Z
modified: 2015-08-31T04:08:45Z
mutable provenance: Salmonella typhimurium
owned by: myers
owned by: james
mutable description: Generates the Salmonella typhimurium Hin protein. This protein is used to invert DNA that is flanked by Hix siteds.
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 918
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none really
star: false
creator: Erin Zwack, Sabriya Rosemond
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Fwd primer J31001
sequence annotation: Hin sequence
sequence annotation: Former SpeI site
sequence annotation: Mutation to delete the SpeI site
sequence annotation: Rev primer J31001
sequence: https://synbiohub.org/public/igem/BBa_J31000_sequence/1

Fwd primer J31001
type: SequenceAnnotation
persistent identity: annotation1910568
display id: annotation1910568
version: 1
top level: Hin
location: https://synbiohub.org/public/igem/BBa_J31000/annotation1910568/range1910568/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_J31000/annotation1910568/range1910568/1
type: Range
persistent identity: range1910568
display id: range1910568
version: 1
top level: Hin
direction: direction/forward
start: 1
end: 3
orientation: inline

Hin sequence
type: SequenceAnnotation
persistent identity: annotation1884987
display id: annotation1884987
version: 1
top level: Hin
location: https://synbiohub.org/public/igem/BBa_J31000/annotation1884987/range1884987/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J31000/annotation1884987/range1884987/1
type: Range
persistent identity: range1884987
display id: range1884987
version: 1
top level: Hin
direction: direction/forward
start: 1
end: 573
orientation: inline

Former SpeI site
type: SequenceAnnotation
persistent identity: annotation1880459
display id: annotation1880459
version: 1
top level: Hin
location: https://synbiohub.org/public/igem/BBa_J31000/annotation1880459/range1880459/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_J31000/annotation1880459/range1880459/1
type: Range
persistent identity: range1880459
display id: range1880459
version: 1
top level: Hin
direction: direction/forward
start: 70
end: 75
orientation: inline

Mutation to delete the SpeI site
type: SequenceAnnotation
persistent identity: annotation1880460
display id: annotation1880460
version: 1
top level: Hin
location: https://synbiohub.org/public/igem/BBa_J31000/annotation1880460/range1880460/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_J31000/annotation1880460/range1880460/1
type: Range
persistent identity: range1880460
display id: range1880460
version: 1
top level: Hin
direction: direction/forward
start: 72
end: 72
orientation: inline

Rev primer J31001
type: SequenceAnnotation
persistent identity: annotation1910569
display id: annotation1910569
version: 1
top level: Hin
location: https://synbiohub.org/public/igem/BBa_J31000/annotation1910569/range1910569/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_J31000/annotation1910569/range1910569/1
type: Range
persistent identity: range1910569
display id: range1910569
version: 1
top level: Hin
direction: direction/reverse
start: 551
end: 570
orientation: inline

BBa_K200021
type: ComponentDefinition
persistent identity: BBa K200021
display id: BBa_K200021
version: 1
was generated by: iGEM to SBOL conversion
description: pLacI+RBS
created: 2009-10-14T11:00:00Z
modified: 2015-05-08T01:11:21Z
mutable provenance: Both parts from the Registry
owned by: james
owned by: myers
mutable description: Promoter for the LacI gene ligated to a RBS
discontinued: false
dominant: false
group u list: _298_
m user id: 0
owner id: 5155
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: Royah Vaezi
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0034
component: BBa_R0011
sequence annotation: BBa_R0011
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K200021_sequence/1

BBa_B0034
type: Component
persistent identity: component2041376
display id: component2041376
version: 1
top level: BBa_K200021
definition: BBa_B0034
access: public

BBa_R0011
type: Component
persistent identity: component2041370
display id: component2041370
version: 1
top level: BBa_K200021
definition: lacI+pL
access: public

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2041370
display id: annotation2041370
version: 1
top level: BBa_K200021
location: https://synbiohub.org/public/igem/BBa_K200021/annotation2041370/range2041370/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K200021/annotation2041370/range2041370/1
type: Range
persistent identity: range2041370
display id: range2041370
version: 1
top level: BBa_K200021
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2041376
display id: annotation2041376
version: 1
top level: BBa_K200021
location: https://synbiohub.org/public/igem/BBa_K200021/annotation2041376/range2041376/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K200021/annotation2041376/range2041376/1
type: Range
persistent identity: range2041376
display id: range2041376
version: 1
top level: BBa_K200021
direction: direction/reverse
start: 64
end: 75
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

lacI
type: ComponentDefinition
persistent identity: BBa C0012
display id: BBa_C0012
version: 1
was generated by: iGEM to SBOL conversion
description: lacI repressor from E. coli (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator <bb_part>BBa_R0010</bb_part> and PLlac01 hybrid regulator <bb_part>BBa_R0011</bb_part> and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription. A rapid degredation tail (LVA) has been added to improve the High to Low performance of this part.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained sequence was compared to the wild-type sequence for LacI obtained through a database search. The sequence had been modified from the wild-type in that wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli has several GTG starts it would seem). The LVA tag has been added for quicker degradation. Incompatible with systems containing LacI, lactose, or IPTG.
star: true
creator: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_C0012
sequence annotation: lacI-LVA
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0012_sequence/1

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation7031
display id: annotation7031
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
type: Range
persistent identity: range7031
display id: range7031
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

lacI-LVA
type: SequenceAnnotation
persistent identity: annotation1723
display id: annotation1723
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
type: Range
persistent identity: range1723
display id: range1723
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1722
display id: annotation1722
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
type: Range
persistent identity: range1722
display id: range1722
version: 1
top level: lacI
direction: direction/forward
start: 1090
end: 1128
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213988
display id: annotation2213988
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
type: Range
persistent identity: range2213988
display id: range2213988
version: 1
top level: lacI
direction: direction/forward
start: 1129
end: 1153
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

https://synbiohub.org/public/igem/BBa_K318015_sequence/1
type: Sequence
persistent identity: BBa K318015 sequence
display id: BBa_K318015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaaaaaaaccccgcccctgacagggcggggtttttttttactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatggctactattgggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttaccagtgcaaattgtgaccgcatttttgaagaccgtatcagtggcaagattgcaaaccgccccggcctgaaacgggcgttaaagtatgtaaataaaggcgatactcttgtcgtctggaaattagacagactgggccgtagcgtgaaaaatctggtggcgttaatatcagaattacatgaacgtggagctcacttccattctttaaccgatagtattgataccagtagcgcgatggggcgattcttttttcatgtaatgtcagcactggccgagatggagcgagaattaatcgtcgagcgaacccttgccggactggctgccgccagagcgcaaggacgactgggagggcgccctcgggcgatcaacaaacatgaacaggaacagattagtcggctattagagaaaggccatcctcggcagcaattagctattatttttggtattggcgtatccaccttatacagatactttccggcaagcagtataaaaaaacgaatgaattaatactagagaaaaaaaaaccccgcccctgacagggcggggtttttttttactagagttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaaaaaaaaccccgcccctgac…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S03520_sequence/1
type: Sequence
persistent identity: BBa S03520 sequence
display id: BBa_S03520_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23100_sequence/1
type: Sequence
persistent identity: BBa J23100 sequence
display id: BBa_J23100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B1006_sequence/1
type: Sequence
persistent identity: BBa B1006 sequence
display id: BBa_B1006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaaaaaaaccccgcccctgacagggcggggtttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J31000_sequence/1
type: Sequence
persistent identity: BBa J31000 sequence
display id: BBa_J31000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctactattgggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttaccagtgcaaattgtgaccgcatttttgaagaccgtatcagtggcaagattgcaaaccgccccggcctgaaacgggcgttaaagtatgtaaataaaggcgatactcttgtcgtctggaaattagacagactgggccgtagcgtgaaaaatctggtggcgttaatatcagaattacatgaacgtggagctcacttccattctttaaccgatagtattgataccagtagcgcgatggggcgattcttttttcatgtaatgtcagcactggccgagatggagcgagaattaatcgtcgagcgaacccttgccggactggctgccgccagagcgcaaggacgactgggagggcgccctcgggcgatcaacaaacatgaacaggaacagattagtcggctattagagaaaggccatcctcggcagcaattagctattatttttggtattggcgtatccaccttatacagatactttccggcaagcagtataaaaaaacgaatgaattaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K200021_sequence/1
type: Sequence
persistent identity: BBa K200021 sequence
display id: BBa_K200021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0012_sequence/1
type: Sequence
persistent identity: BBa C0012 sequence
display id: BBa_C0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z