LOCUS       BBa_K318020             2323 bp    DNA     linear       23-Dec-2024
DEFINITION  T + araC + pBad + RBS + Cre
ACCESSION   BBa_K318020
VERSION     BBa_K318020.1  
FEATURES             Location/Qualifiers
     misc_feature    1..39
                     /label=B1006
     stem_loop       10..31
                     /label=modified thr terminator
     polyA_site      32..39
                     /label=PolyA
     polyA_site      1..9
                     /label=PolyA
     terminator      1..39
                     /label=BBa_B1006
     promoter        1127..1257
                     /label=Pbad
     promoter        1090..1161
                     /label=PC
     misc_feature    48..1126
                     /label=AraC
     promoter        48..1257
                     /label=BBa_I0500
     RBS             1266..1280
                     /label=RBS-1\Strong
     RBS             1273..1277
                     /label=RBS
     misc_feature    1266..1280
                     /label=BBa_B0030
     RBS             1266..1280
                     /label=BBa_B0030
     CDS             1287..2323
                     /label=BBa_J61047
ORIGIN
        1 aaaaaaaaac cccgcccctg acagggcggg gttttttttt actagagtta tgacaacttg
       61 acggctacat cattcacttt ttcttcacaa ccggcacgga actcgctcgg gctggccccg
      121 gtgcattttt taaatacccg cgagaaatag agttgatcgt caaaaccaac attgcgaccg
      181 acggtggcga taggcatccg ggtggtgctc aaaagcagct tcgcctggct gatacgttgg
      241 tcctcgcgcc agcttaagac gctaatccct aactgctggc ggaaaagatg tgacagacgc
      301 gacggcgaca agcaaacatg ctgtgcgacg ctggcgatat caaaattgct gtctgccagg
      361 tgatcgctga tgtactgaca agcctcgcgt acccgattat ccatcggtgg atggagcgac
      421 tcgttaatcg cttccatgcg ccgcagtaac aattgctcaa gcagatttat cgccagcagc
      481 tccgaatagc gcccttcccc ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa
      541 tgcggctggt gcgcttcatc cgggcgaaag aaccccgtat tggcaaatat tgacggccag
      601 ttaagccatt catgccagta ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg
      661 cgagcctccg gatgacgacc gtagtgatga atctctcctg gcgggaacag caaaatatca
      721 cccggtcggc aaacaaattc tcgtccctga tttttcacca ccccctgacc gcgaatggtg
      781 agattgagaa tataaccttt cattcccagc ggtcggtcga taaaaaaatc gagataaccg
      841 ttggcctcaa tcggcgttaa acccgccacc agatgggcat taaacgagta tcccggcagc
      901 aggggatcat tttgcgcttc agccatactt ttcatactcc cgccattcag agaagaaacc
      961 aattgtccat attgcatcag acattgccgt cactgcgtct tttactggct cttctcgcta
     1021 accaaaccgg taaccccgct tattaaaagc attctgtaac aaagcgggac caaagccatg
     1081 acaaaaacgc gtaacaaaag tgtctataat cacggcagaa aagtccacat tgattatttg
     1141 cacggcgtca cactttgcta tgccatagca tttttatcca taagattagc ggatcctacc
     1201 tgacgctttt tatcgcaact ctctactgtt tctccatacc cgtttttttg ggctagctac
     1261 tagagattaa agaggagaaa tactagatgt ccaatttact gaccgtacac caaaatttgc
     1321 ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg gacatgttca
     1381 gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt tgccggtcgt
     1441 gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct gaagatgttc
     1501 gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaactatc cagcaacatt
     1561 tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt gacagcaatg
     1621 ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt gaacgtgcaa
     1681 aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc atggaaaata
     1741 gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat aacaccctgt
     1801 tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact gacggtggga
     1861 gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt gtagagaagg
     1921 cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct ggtgtagctg
     1981 atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc gcgccatctg
     2041 ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact catcgattga
     2101 tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga cacagtgccc
     2161 gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag atcatgcaag
     2221 ctggtggctg gaccaatgta aatattgtca tgaactatat ccgtaacctg gatagtgaaa
     2281 caggggcaat ggtgcgcctg ctggaagatg gcgattaaga att
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