##gff-version 3
##sequence-region BBa_K318022 1 701
BBa_K318022	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K318022	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K318022	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K318022	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K318022	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K318022	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K318022	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K318022	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K318022	.	CDS	82	654	.	+	0	ID=BBa_J31000;Name=BBa_J31000
BBa_K318022	.	CDS	82	654	.	+	0	ID=annotation1884987;Name=Hin sequence;Parent=BBa_J31000
BBa_K318022	.	sequence_alteration	153	153	.	+	0	ID=annotation1880460;Name=Mutation to delete the SpeI site;Parent=BBa_J31000
BBa_K318022	.	sequence_alteration	151	156	.	+	0	ID=annotation1880459;Name=Former SpeI site;Parent=BBa_J31000
BBa_K318022	.	primer_binding_site	632	651	.	+	0	ID=annotation1910569;Name=Rev primer J31001;Parent=BBa_J31000
BBa_K318022	.	primer_binding_site	82	84	.	+	0	ID=annotation1910568;Name=Fwd primer J31001;Parent=BBa_J31000
BBa_K318022	.	terminator	663	701	.	+	0	ID=BBa_B1006;Name=BBa_B1006
BBa_K318022	.	polyA_site	694	701	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K318022	.	stem_loop	672	693	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K318022	.	polyA_site	663	671	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K318022	.	engineered_region	663	701	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
>BBa_K318022
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatggctactattgggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttaccagtgc
aaattgtgaccgcatttttgaagaccgtatcagtggcaagattgcaaaccgccccggcctgaaacgggcgttaaagtat
gtaaataaaggcgatactcttgtcgtctggaaattagacagactgggccgtagcgtgaaaaatctggtggcgttaatat
cagaattacatgaacgtggagctcacttccattctttaaccgatagtattgataccagtagcgcgatggggcgattctt
ttttcatgtaatgtcagcactggccgagatggagcgagaattaatcgtcgagcgaacccttgccggactggctgccgcc
agagcgcaaggacgactgggagggcgccctcgggcgatcaacaaacatgaacaggaacagattagtcggctattagaga
aaggccatcctcggcagcaattagctattatttttggtattggcgtatccaccttatacagatactttccggcaagcag
tataaaaaaacgaatgaattaatactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt