BBa_I11030 1 BBa_I11030 integrase from E. coli phage P22 2004-09-02T11:00:00Z 2015-08-31T04:07:30Z Nuc Acid Res, 2001; 29(11):2205-2216 P22 Integrase with LVA tag false false _2_ 0 102 7 It's complicated false false mschomp annotation1126751 1 LVA range1126751 1 1162 1194 annotation2214028 1 Help:Barcodes range2214028 1 1201 1225 annotation1126806 1 stop range1126806 1 1195 1200 annotation1126718 1 Integrase p22 range1126718 1 1 1161 BBa_K318023 1 BBa_K318023 pLac + RBS + Int (P22) 2010-07-12T11:00:00Z 2015-05-08T01:11:57Z biobricks This composite part is an inducible P22 Int DNA integrase generator which acts on the attB and attP recombination sites. It is composed of the following biobricks: BBa_K200021 and BBa_I11030. Note that to fully repress the pLac promoter, overproduction of the lacI repressor protein is necessary. false false _438_ 0 4845 9 Not in stock false Note that to fully repress the pLac promoter, overproduction of the lacI repressor protein is necessary. false Nathaniel Pantalone, Justin Vrana component2247158 1 BBa_I11030 component2247153 1 BBa_K200021 annotation2247153 1 BBa_K200021 range2247153 1 1 75 annotation2247158 1 BBa_I11030 range2247158 1 82 1306 BBa_K200021 1 BBa_K200021 pLacI+RBS 2009-10-14T11:00:00Z 2015-05-08T01:11:21Z Both parts from the Registry Promoter for the LacI gene ligated to a RBS false false _298_ 0 5155 9 It's complicated false N/A false Royah Vaezi component2041376 1 BBa_B0034 component2041370 1 BBa_R0011 annotation2041370 1 BBa_R0011 range2041370 1 1 54 annotation2041376 1 BBa_B0034 range2041376 1 64 75 BBa_R0011 1 lacI+pL Promoter (lacI regulated, lambda pL hybrid) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z represillator of Elowitz and Leibler (2000) Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference). false true _1_ 0 24 7 In stock false <P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs. true Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton annotation2000 1 -35 range2000 1 20 25 annotation2001 1 lac O1 range2001 1 26 42 annotation7064 1 BBa_R0011 range7064 1 1 54 annotation2002 1 -10 range2002 1 43 48 annotation1999 1 lac O1 range1999 1 3 19 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K318023_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgtcactattccgcagaggtgaaacctggtacgccagtttcacattgccgaacggcaaaagatttaagcagtctcttgggacaaaggacaaaaggcaggccacagagcttcatgacaagctgaaggcagaagcatggagggtaagtaaattaggagagacgcctgacatgacttttgagggggcctgtgtcaggtggttagaggagaaggcgcataagaagtcgctggatgatgacaagagtcggataggattctggctccagcattttgcaggaatgcagttgaaggatattaccgagacgaagatttactccgccatccagaagataactaatcggcggcatgaggaaaactggaagttaatggatgaagcatgcaggaagaatgggaagcagcctccggtattcaagcctaagccggcagcagtagctacaaaagcaactcacctttcattcattaaggcactcctccgggctgctgaacgcgaatggaagatgctggataaggctccgatcatcaaagttcctcagccgaaaaataagcgtatccgctggcttgagcctcacgaggcaaaaaggttgattgatgaatgccaggaaccgctaaagtcagtcgtagagtttgcgctttctactggcttaaggcggtctaacattatcaatctggagtggcagcagatagacatgcaacgaaaggtggcatggatacacccggaacaaagcaagtctaatcatgccattggagtggcgctgaatgataccgcttgccgggtgctgaaaaagcaaatcggcaatcatcacaaatgggtgttcgtctacaaggaaagcagcaccaaaccagacggaactaaatcacctgtagtgaggaagatgcgctatgacgctaatactgcatggagggcagcattaaaacgagcgggcattgaagacttccgttttcatgacctgaggcacacgtgggcaagttggttagttcaggctggcgttccgatttcggtattgcaggaaatgggtggctgggagtctatcgaaatggttcgccgatatgctcatctggcaccaaatcacctgactgaacatgctcgacaaattgactcgatttttggtacttctgtcccaaatatgtcccacagtaaaaataaggaaggcacgaataatacggcagcaaacgacgaaaactacgctttagtagcttaataaccctgatagtgctagtgtagatccc BBa_B0034_sequence 1 aaagaggagaaa BBa_K200021_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaa BBa_R0011_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca BBa_I11030_sequence 1 atgtcactattccgcagaggtgaaacctggtacgccagtttcacattgccgaacggcaaaagatttaagcagtctcttgggacaaaggacaaaaggcaggccacagagcttcatgacaagctgaaggcagaagcatggagggtaagtaaattaggagagacgcctgacatgacttttgagggggcctgtgtcaggtggttagaggagaaggcgcataagaagtcgctggatgatgacaagagtcggataggattctggctccagcattttgcaggaatgcagttgaaggatattaccgagacgaagatttactccgccatccagaagataactaatcggcggcatgaggaaaactggaagttaatggatgaagcatgcaggaagaatgggaagcagcctccggtattcaagcctaagccggcagcagtagctacaaaagcaactcacctttcattcattaaggcactcctccgggctgctgaacgcgaatggaagatgctggataaggctccgatcatcaaagttcctcagccgaaaaataagcgtatccgctggcttgagcctcacgaggcaaaaaggttgattgatgaatgccaggaaccgctaaagtcagtcgtagagtttgcgctttctactggcttaaggcggtctaacattatcaatctggagtggcagcagatagacatgcaacgaaaggtggcatggatacacccggaacaaagcaagtctaatcatgccattggagtggcgctgaatgataccgcttgccgggtgctgaaaaagcaaatcggcaatcatcacaaatgggtgttcgtctacaaggaaagcagcaccaaaccagacggaactaaatcacctgtagtgaggaagatgcgctatgacgctaatactgcatggagggcagcattaaaacgagcgggcattgaagacttccgttttcatgacctgaggcacacgtgggcaagttggttagttcaggctggcgttccgatttcggtattgcaggaaatgggtggctgggagtctatcgaaatggttcgccgatatgctcatctggcaccaaatcacctgactgaacatgctcgacaaattgactcgatttttggtacttctgtcccaaatatgtcccacagtaaaaataaggaaggcacgaataatacggcagcaaacgacgaaaactacgctttagtagcttaataaccctgatagtgctagtgtagatccc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z