LOCUS       BBa_K318024             1353 bp    DNA     linear       22-Nov-2024
DEFINITION  pLac + RBS + Int (P22) + T
ACCESSION   BBa_K318024
VERSION     BBa_K318024.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    1..75
                     /label=BBa_K200021
     CDS             82..1242
                     /label=Integrase p22
     misc_feature    1243..1275
                     /label=LVA
     misc_feature    1276..1281
                     /label=stop
     misc_feature    1282..1306
                     /label=Help:Barcodes
     CDS             82..1306
                     /label=BBa_I11030
     misc_feature    1315..1353
                     /label=B1006
     stem_loop       1324..1345
                     /label=modified thr terminator
     polyA_site      1346..1353
                     /label=PolyA
     polyA_site      1315..1323
                     /label=PolyA
     terminator      1315..1353
                     /label=BBa_B1006
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgtcacta ttccgcagag gtgaaacctg gtacgccagt
      121 ttcacattgc cgaacggcaa aagatttaag cagtctcttg ggacaaagga caaaaggcag
      181 gccacagagc ttcatgacaa gctgaaggca gaagcatgga gggtaagtaa attaggagag
      241 acgcctgaca tgacttttga gggggcctgt gtcaggtggt tagaggagaa ggcgcataag
      301 aagtcgctgg atgatgacaa gagtcggata ggattctggc tccagcattt tgcaggaatg
      361 cagttgaagg atattaccga gacgaagatt tactccgcca tccagaagat aactaatcgg
      421 cggcatgagg aaaactggaa gttaatggat gaagcatgca ggaagaatgg gaagcagcct
      481 ccggtattca agcctaagcc ggcagcagta gctacaaaag caactcacct ttcattcatt
      541 aaggcactcc tccgggctgc tgaacgcgaa tggaagatgc tggataaggc tccgatcatc
      601 aaagttcctc agccgaaaaa taagcgtatc cgctggcttg agcctcacga ggcaaaaagg
      661 ttgattgatg aatgccagga accgctaaag tcagtcgtag agtttgcgct ttctactggc
      721 ttaaggcggt ctaacattat caatctggag tggcagcaga tagacatgca acgaaaggtg
      781 gcatggatac acccggaaca aagcaagtct aatcatgcca ttggagtggc gctgaatgat
      841 accgcttgcc gggtgctgaa aaagcaaatc ggcaatcatc acaaatgggt gttcgtctac
      901 aaggaaagca gcaccaaacc agacggaact aaatcacctg tagtgaggaa gatgcgctat
      961 gacgctaata ctgcatggag ggcagcatta aaacgagcgg gcattgaaga cttccgtttt
     1021 catgacctga ggcacacgtg ggcaagttgg ttagttcagg ctggcgttcc gatttcggta
     1081 ttgcaggaaa tgggtggctg ggagtctatc gaaatggttc gccgatatgc tcatctggca
     1141 ccaaatcacc tgactgaaca tgctcgacaa attgactcga tttttggtac ttctgtccca
     1201 aatatgtccc acagtaaaaa taaggaaggc acgaataata cggcagcaaa cgacgaaaac
     1261 tacgctttag tagcttaata accctgatag tgctagtgta gatccctact agagaaaaaa
     1321 aaaccccgcc cctgacaggg cggggttttt ttt
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