LOCUS       BBa_K318025              911 bp    DNA     linear       23-Dec-2024
DEFINITION  attB + RBS + GFP + T
ACCESSION   BBa_K318025
VERSION     BBa_K318025.1  
FEATURES             Location/Qualifiers
     misc_feature    1..27
                     /label=BBa_I11032
     RBS             36..48
                     /label=RBS-3\Weak
     RBS             42..45
                     /label=RBS
     misc_feature    36..48
                     /label=BBa_B0032
     RBS             36..48
                     /label=BBa_B0032
     CDS             55..774
                     /label=GFP protein
     CDS             55..774
                     /label=BBa_E0040
     stem_loop       794..837
                     /label=stem_loop
     misc_feature    783..862
                     /label=BBa_B0010
     terminator      783..862
                     /label=BBa_B0010
     stem_loop       878..897
                     /label=T7 TE
     misc_feature    904..904
                     /label=stop
     polyA_site      898..911
                     /label=polya
     misc_feature    871..911
                     /label=BBa_B0012
     terminator      871..911
                     /label=BBa_B0012
ORIGIN
        1 acgaccttcg cattacgaat gcgctgctac tagagtcaca caggaaagta ctagatgcgt
       61 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
      121 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt
      181 acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact
      241 actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac
      301 tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat
      361 gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga
      421 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa
      481 tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa
      541 gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat
      601 caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc
      661 acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag
      721 tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata ataatactag
      781 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
      841 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
      901 ctgcgtttat a
//