LOCUS       BBa_K318500              842 bp    DNA     linear       03-May-2025
DEFINITION  lacI pL + RBS + RcsA + TT
ACCESSION   BBa_K318500
VERSION     BBa_K318500.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..705
                     /label=BBa_K137113
     stem_loop       725..768
                     /label=stem_loop
     misc_feature    714..793
                     /label=BBa_B0010
     terminator      714..793
                     /label=BBa_B0010
     stem_loop       809..828
                     /label=T7 TE
     misc_feature    835..835
                     /label=stop
     polyA_site      829..842
                     /label=polya
     misc_feature    802..842
                     /label=BBa_B0012
     terminator      802..842
                     /label=BBa_B0012
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgtcaacg attattatgg atttatgtag ttacacccga
      121 ctaggtttaa ccgggtatct gttgagtaga ggggttaaaa aaagagaaat caacgacatt
      181 gaaaccgttg atgaccttgc catagcttgt gattcacagc gcccttcagt ggtgtttatt
      241 aatgaggact gtttcatcca cgatgcttct aacagtcagc gtatcaagct catcattaat
      301 caacatccca atacgttatt tatcgttttt atggcaattg ccaatgttca ttttgatgaa
      361 tatctattgg tcagaaaaaa tttattgatc agttctaaat cgattaaacc ggaatctctc
      421 gacgatatcc ttggcgatat tctgaaaaaa gagacaacga taacctcgtt tttaaatatg
      481 ccgacgttat cattgagccg aaccgaatcg agtatgttgc gaatgtggat ggcaggtcag
      541 ggaaccattc aaatctctga ccaaatgaat atcaaagcca agaccgtttc atcgcataaa
      601 ggtaatatta aacgtaagat caaaacgcat aataaacagg ttatctacca tgtcgtccga
      661 ctgacggata atgtgactaa tggtattttt gtcaacatgc gctaatacta gagccaggca
      721 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
      781 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
      841 ta
//