LOCUS       BBa_K318501              872 bp    DNA     linear       22-Nov-2024
DEFINITION  lacI pL + RBS + RcsB + TT
ACCESSION   BBa_K318501
VERSION     BBa_K318501.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..729
                     /label=RcsB gene
     misc_feature    82..84
                     /label=start
     misc_feature    730..734
                     /label=stop
     CDS             82..735
                     /label=BBa_K200000
     stem_loop       755..798
                     /label=stem_loop
     misc_feature    744..823
                     /label=BBa_B0010
     terminator      744..823
                     /label=BBa_B0010
     stem_loop       839..858
                     /label=T7 TE
     misc_feature    865..865
                     /label=stop
     polyA_site      859..872
                     /label=polya
     misc_feature    832..872
                     /label=BBa_B0012
     terminator      832..872
                     /label=BBa_B0012
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgaacaat atgaacgtaa ttattgccga tgaccatccg
      121 atagtcttgt tcggtattcg caaatcactt gagcaaattg agtgggtgaa tgttgtcggc
      181 gaatttgaag actctacagc actgatcaac aacctgccga aactggatgc gcatgtgttg
      241 attaccgatc tctccatgcc tggcgataag tacggcgatg gcattacctt aatcaagtac
      301 atcaagcgcc atttcccaag cctgtcgatc attgttctga ctatgaacaa caacccggcg
      361 attcttagtg cggtattgga tctggatatc gaagggatcg tgctgaaaca aggtgcaccg
      421 accgatctgc cgaaagctct cgccgcgctc cagaaaggga agaaatttac cccggaaagc
      481 gtttctcgcc tgttggaaaa aatcagtgct ggtggttacg gtgacaagcg tctctcgcca
      541 aaagagagtg aagttctgcg cctgtttgcg gaaggcttcc tggtgaccga gatcgctaaa
      601 aagctgaacc gcagtattaa aaccatcagt agccagaaga aatctgcgat gatgaagctg
      661 ggtgtcgaga acgatatcgc cctgctgaat tatctctctt cagtgacctt aagtccggca
      721 gataaagact aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
      781 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
      841 ctcaccttcg ggtgggcctt tctgcgttta ta
//