LOCUS       BBa_K318502             1522 bp    DNA     linear       05-Nov-2024
DEFINITION  lacI pL + RBS + RcsB + RBS + RcsA + TT
ACCESSION   BBa_K318502
VERSION     BBa_K318502.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..729
                     /label=RcsB gene
     misc_feature    82..84
                     /label=start
     misc_feature    730..734
                     /label=stop
     CDS             82..735
                     /label=BBa_K200000
     misc_feature    748..751
                     /label=conserved
     RBS             744..755
                     /label=BBa_B0034
     CDS             762..1385
                     /label=BBa_K137113
     stem_loop       1405..1448
                     /label=stem_loop
     misc_feature    1394..1473
                     /label=BBa_B0010
     terminator      1394..1473
                     /label=BBa_B0010
     stem_loop       1489..1508
                     /label=T7 TE
     misc_feature    1515..1515
                     /label=stop
     polyA_site      1509..1522
                     /label=polya
     misc_feature    1482..1522
                     /label=BBa_B0012
     terminator      1482..1522
                     /label=BBa_B0012
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgaacaat atgaacgtaa ttattgccga tgaccatccg
      121 atagtcttgt tcggtattcg caaatcactt gagcaaattg agtgggtgaa tgttgtcggc
      181 gaatttgaag actctacagc actgatcaac aacctgccga aactggatgc gcatgtgttg
      241 attaccgatc tctccatgcc tggcgataag tacggcgatg gcattacctt aatcaagtac
      301 atcaagcgcc atttcccaag cctgtcgatc attgttctga ctatgaacaa caacccggcg
      361 attcttagtg cggtattgga tctggatatc gaagggatcg tgctgaaaca aggtgcaccg
      421 accgatctgc cgaaagctct cgccgcgctc cagaaaggga agaaatttac cccggaaagc
      481 gtttctcgcc tgttggaaaa aatcagtgct ggtggttacg gtgacaagcg tctctcgcca
      541 aaagagagtg aagttctgcg cctgtttgcg gaaggcttcc tggtgaccga gatcgctaaa
      601 aagctgaacc gcagtattaa aaccatcagt agccagaaga aatctgcgat gatgaagctg
      661 ggtgtcgaga acgatatcgc cctgctgaat tatctctctt cagtgacctt aagtccggca
      721 gataaagact aataatacta gagaaagagg agaaatacta gatgtcaacg attattatgg
      781 atttatgtag ttacacccga ctaggtttaa ccgggtatct gttgagtaga ggggttaaaa
      841 aaagagaaat caacgacatt gaaaccgttg atgaccttgc catagcttgt gattcacagc
      901 gcccttcagt ggtgtttatt aatgaggact gtttcatcca cgatgcttct aacagtcagc
      961 gtatcaagct catcattaat caacatccca atacgttatt tatcgttttt atggcaattg
     1021 ccaatgttca ttttgatgaa tatctattgg tcagaaaaaa tttattgatc agttctaaat
     1081 cgattaaacc ggaatctctc gacgatatcc ttggcgatat tctgaaaaaa gagacaacga
     1141 taacctcgtt tttaaatatg ccgacgttat cattgagccg aaccgaatcg agtatgttgc
     1201 gaatgtggat ggcaggtcag ggaaccattc aaatctctga ccaaatgaat atcaaagcca
     1261 agaccgtttc atcgcataaa ggtaatatta aacgtaagat caaaacgcat aataaacagg
     1321 ttatctacca tgtcgtccga ctgacggata atgtgactaa tggtattttt gtcaacatgc
     1381 gctaatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     1441 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
     1501 ggtgggcctt tctgcgttta ta
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